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iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns by Bio-Rad

Manufacturer Bio-Rad  |   Model: 1708890  |  Available Worldwide
4.8
/
5.0
  |  3 reviews
25 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water


iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns by Bio-Rad product image
iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
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Average Rating: 4.8
3 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Advanced Reviewer
  • Member since: 2011

  • Organization: MSKCC



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Easy to use, great yield every time, painless
Rating: 4.7

  • Application Area: RT-PCT, qRT-PCR, etc

"Very easy to use, works every time, almost effortless. Kit shelf life is extremely long, never has a problem, best bang for the money!"

Review date: 03 Nov 2017 | iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
  • Status:

    Expert Reviewer

  • Member since: 2015

  • Organization: Postdoctoral Associate in Cambridge MA



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great for most cDNA for qPCR
Rating: 4.7

  • Application Area:cDNA for qPCR

"Great kit for the synthesis of cDNA, is very consistent. However, if you are utilizing a small amount of RNA this kit isn't that great. Would recommend a more sensitive RT."

Review date: 15 Jan 2016 | iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: Ohio State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0

Review date: 29 Sep 2015 | iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns

Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.

Key Features and Benefits

  • Up to 15 µl of RNA in a 20 µl cDNA synthesis reaction
  • Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
  • Fast 26 min cDNA synthesis protocol
  • Linear range of 1 µg–1 pg total input RNA using RNase H+ MMLV reverse transcriptase


See the iScript cDNA Synthesis Kit product page for more information and documentation.

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Related Categories

  • PCR and qPCR Reagents
  • Real-Time PCR Detection Systems
  • PCR Plastic Consumables
  • Instrument Validation Tools and Services

Online Resources

  • qPCR/Real-Time PCR
  • Genomics

Product Overview

iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns by Bio-Rad product image

iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns

Manufacturer Bio-Rad  |  Available Worldwide

4.8 / 5.0 | 3 reviews