HALO® PCS C18 column

In this application note from Advanced Materials Technology, learn more about the new HALO® PCS C18 phase which offers an alternative to chromatographic separations under acidic conditions. Furthermore, explore how the new line of HALO® PCS C18 columns are built on Fused-Core® technology so that chromatographers can trust in fast and efficient separations of basic compounds in both small molecule and biological separations.

In this application note from Advanced Materials Technology, explore how a separation of six different insulins, both natural and recombinant, is performed on the HALO 160 Å PCS C18 column. Each insulin is very similar in chemical makeup with only slight changes in each peptide. With the use of the positively charged surface stationary phase and the 160 Å pore size, each insulin form can be separated under isocratic conditions. This is important for insulin manufacturers to confirm their quality control of the product. If small changes in the formulations are not monitored correctly, then there is a chance of severe allergic reaction to the drug. The HALO 160 Å PCS C18 column can help monitor these drugs through the quality control process and help deliver safe drugs to a patient.

In this application note from Advanced Materials Technology, explore how the HALO 160 Å PCS C18 column outperforms a traditional C18 column under formic acid conditions due to its positive charge surface. This allows for improved peak shape and resolution for peptides. PCS C18 also allows for a higher sample load on column for basic analytes and could potentially help pull apart closely retained impurities.

Screening for pesticides is required for cannabis testing in Oregon. The Oregon pesticide list consists of 59 compounds and two of these are isomers (spinosad A and D and pyrethrin I and II). In this application note from Advanced Materials Technology, explore how all of the compounds were run using positive ESI except fludioxinil and fipronil, which were run in negative mode since it is easier for these compounds to be oxidized. A HALO PCS C18 column was used to highlight the symmetrical peak shapes obtained when using formic acid modified mobile phase. The entire analysis including re-equilibration is 12 minutes, which highlights the speed of Fused-Core® analysis.

Beta blockers are used for the treatment and/or prevention of heart and circulatory conditions, such as arrhythmias, heart attack, and high blood pressure. In this application note from Advanced Materials Technology, explore how eleven different beta blockers are separated in under five minutes using the HALO® PCS C18 column with UV detection and a mobile phase that is MS compatible.

In this application note from Advanced Materials Technology, explore how a synthetic peptide panel is screened on 160 Å PCS C18 compared to a C18 stationary phase. While using low ionic strength mobile phases such as formic acid the positively charged surface stationary phase shows narrower peak widths and improved peak asymmetry when compared to a traditional C18 stationary phase without endcapping.

In this application note, Advanced Materials Technology discusses the utilization of a HALO® 90 Å PCS C18 column to separate tricyclic antidepressants (TCA), a drug class mainly employed in depression management. The positively charged surface (PCS) stationary phase proves advantageous for basic analytes with low ionic strength mobile phases, like formic acid. Additionally, it enhances sample loading in contrast to conventional C18 columns available in the market.

In this application note, Advanced Materials Technology introduces the utilization of a HALO® PCS C18 with Fused-Core® for speed and efficiency. This positively charged surface chemistry is engineered to enhance peak shapes for basic compounds. HALO® PCS is well-suited for low ionic strength mobile phases, maintaining peak symmetry even at higher loading capacities, and offering distinct selectivity compared to other C18 bonded phases. It comes in both Å and 160 Å pore sizes for the analysis of small molecules and peptides.