Status:
ReviewerMember since: 2023
Organization: UCLA
Great results helped me advance my experiment quickly!
Application Area: Study protein expression in mammalian cells
"The DNA constructs were as designed and allowed me to easily clone into my target vector. Protein expression was detected in HEK293T cells. The company is very supportive online I would definitely recommend!"
Status:
ReviewerMember since: 2023
Organization: University of Pennsylvania
Highly recommend!
Application Area:cloning
"First time ordering from TwistBioscience and everything went great. Ordered 8 gene fragments and they arrived one day earlier than the expected delivery date. Used all gene fragments directly with NEBuilder HIFI assembly for cloning and worked great (sequences of the cloned constructs have been confirmed with Sanger sequencing). Would definitely recommend for the price, turnaround time, and quality."
Status:
ReviewerMember since: 2023
Organization: VIB
Best cloning aid!
Application Area:Cloning
"I needed to synthesize gene fragments with repetitive DNA sequences (i.e. 5aa linker in tandem) and TWIST was the only company that allowed me to do it. The ordering process was easy and straightforward (loading the sequences in bulk, correcting according to the company suggestions/requisites), and the price is very competitive. Once I got them, 90% of the tested colonies had my sequence of interest. All good!"
Status:
ReviewerMember since: 2023
Organization: HHMI
Great service even if sequence submission can be time-consuming
Application Area:Designing homology arms for CRISPR
"TWIST is excellent for producing the correct sequences you want with an impressive turnaround time of ~7 days. The price is also very competitive and transparent. The only downside is the slightly clunky sequence submission platform. You will find yourself going back and forth with your desired sequence changing the GC content in some areas and avoiding repeat regions. Also be sure to give your file a short name as the system does not deal with long names. Besides this, ordering is very straight forward and my interactions with customer service reps has also been very good."
Status:
ReviewerMember since: 2023
Organization: Industry
Cost effective good quality gene fragments!
Application Area:Molecular Biology
"Our company has been very happy with Twist gene fragments and clonal genes. They're comparable to the other synthesis companies in terms of application with Gibson and other cloning methods and sometimes see point mutations but out of 6 clones it's usually plenty to find a correct insert. Their clonal genes make streamlining cloning much easier and the few genes that gave very low yield seemed problematic in E. coli so they recloned it in a low copy number plasmid for us. Their cost is extremely competitive and they're our go-to for gene fragments as long as the sequence passes their algorithm."
Status:
ReviewerMember since: 2023
Organization: University of Zurich
Fast, cheap, and reliable source for gene fragment synthesis for cloning
Application Area:Cloning genes and viral construct development
"Twist is incredibly fast, cheap, and accurate producers of completely custom DNA sequences. Even though they’re based in San Francisco, I received my gene fragments within two weeks of ordering, all with perfect sequences."
Status:
ReviewerMember since: 2022
Organization: UCD
Reliable service; will recommend to my colleagues who need synthetic DNA service.
Application Area:gene subcloning
"First time to order gene fragment from Twist. The platform for submitting sequence and error-checking is friendly and straightforward. Fantastic service far beyond my expectation. The price and turnaround time cannot be beat, while the gene product is flawless. The guaranteed minimum 1000 ng of gene fragment are more than sufficient for restriction enzyme digestion, purification and subsequent ligation reaction. Strongly recommend Twist if you need service for synthetic DNA fragment or clone."
Status:
ReviewerMember since: 2022
Organization: The University of Texas Houston Medical School
Great service and fast turnover time
Application Area:Gene synthesis
"Twist Biosciences is my go-to service provider for gene synthesis. The speed is fast! I receive my gene fragment within one week. The gene optimization program online is easy to use. Highly recommended."
Status:
ReviewerMember since: 2022
Organization: ETH
Happy customer
Application Area:DNA synthesis
"Quick and cost effective service, in case of questions they generally reply to emails within one day."
Status:
ReviewerMember since: 2022
Organization: Oxford University
Excellent, cannot find fault with this company
Application Area:Cloning for Transformation of Genes into Plants
"The gene rragments worked really well, The service from Twist Bioscience was excellent. We use Twist all the time now for my gene fragments."
Status:
ReviewerMember since: 2022
Organization: Stanford Biology Department
Great product. Best prices available and the gene fragments were
Application Area:Synthetic DNA assembly
"The interface was easy to use. Twist sent me the ordered product in a reasonable amount of time, and was actually faster than their estimated date. I PCR'd and sequenced one of the gene fragments I ordered due to high complexity, and the gel purified band of the correct size was sequence perfect. The only small issue was that the amount of DNA varied between tubes by around 30-50% of the expected value(some were higher, some were lower), despite asking for normalization on their end. I still had more than enough for golden gate reactions."
Status:
ReviewerMember since: 2022
Organization: Uppsala University
Great product, it has definitely saved my project!
Application Area:Homologous recombination, cloning, molecular biology
"I was really satisfied with my order. First of all, the platform is really easy use. Then, I was amazed on how fast the gene fragments were synthesised. The order arrived 2 days before we expected and given the high quality of the products we build a library that otherwise would have taken months to be ready."
Status:
ReviewerMember since: 2022
Organization: CHUV
We have had no issues with the sequence so far.
Application Area:CAR T cell manufacturing
"They offer a very good service, with friendly user interface, clearly stating what is needed to improve on your orders, and offering a variety of options. Price-wise, it could be slightly better as for shorter sequences or a lower amount of gene fragments ordered makes the price per base notably increased."
Status:
ReviewerMember since: 2021
Organization: BPS Bioscience
Great quality, low cost, saves time, easy to use.
Application Area:Gene fragment (dsDNA) for cloning / recombination
"Twist is extremely easy to use and provides the lowest cost for gene fragment compared with their competitors. The product quality is always perfect and as advertised. The pricing makes it possible to take on much more ambitious goals and their option to clone the genes for us adds even more possibility for increased impact. We have found that it is rarely cost effective to clone in-house anymore, and when it is, we can often still use gene fragments to speed the process along and give us more options. Their vector onboarding allows us to use our in-house vectors for their clonal gene service, but most of the time their existing vector options are perfect for our applications. The genes take around 3 weeks to arrive, so we plan further in advance and take on more projects during the meantime. Their website and UI is extremely intuitive and easy to work with, unlike many competitor websites. I love working with Twist and I go to them first for any cloning project I have."
Status:
ReviewerMember since: 2022
Organization: TBI - INSA Toulouse
Easy and fast to order ! Good synthetized DNA quality !
Application Area:Protein interaction study
"The order platform is very easy to use. The order was quickly placed and started to be managed. The synthetized DNA is correct in sequence even for long fragments. Flanking adaptators allow you to amplify your DNA if need be. Order has arrived on time !"
Status:
ReviewerMember since: 2022
Organization: Gilead
Great product, great quality, fats turnaround and cheapest pricing.
Application Area:Analyze gene fragments in display based approaches
"The quality of Twist product is very good. I have ordered gene fragments from other vendors in the past but have had high failure rates and longer than published turn around time. Ordering with Twist, I have had much better overall synthesis success rate and the turnaround time is usually very fast. Not to mention, this is the cheapest pricing for gene fragments. I have switched to using Twist for all my gene synthesis needs."
Status:
ReviewerMember since: 2022
Organization: Truebinding
Highly recommend! Twist checks all the boxes!
Application Area:Gene fragment synthesis with overhangs that are compatible to our vector for assembly cloning
"I have worked in Molecular Biology for over 20 years and have pretty much seen it all in terms of experience with different vendors. I can confidently say that Twist checks ALL the boxes. In our recent interaction, the most important box checked was the high sequence accuracy of the fragments we ordered. With a 94% sequence accuracy rate, we did not have to go back to pick additional colonies for any of our 48 constructs. The ordering process was simple and turnaround times and prices are highly competitive. Fragments are synthesized in the United States so the shipping is faster with no risk of potential customs delays. I have had positive interactions with everyone I’ve communicated with at Twist and the customer service has been great! There was even follow-up to make sure we were satisfied with our order. I’m very appreciative of Twist and the top-notch products, services, and technologies they have to offer!"
Status:
ReviewerMember since: 2022
Organization: Cornell University
High quality product, would order from Twist again
Application Area:Protein expression
"Gene fragments were of high quality. The order process is smooth and easy to navigate. Very reasonable prices and excellent customer service!"
Status:
ReviewerMember since: 2022
Organization: Geetanjali institute of technology
Great result.
Application Area:Twist bioscience gene fragment improve our cloning process by minimising colony screening
"This allows us to save time and money."
Status:
ReviewerMember since: 2022
Organization: UC-Santa Barbara
Twist gene fragments are great value and easy to use.
Application Area:Yeast cloning
"I ordered gene fragments from Twist to clone a number of point mutants of a gene of interest into a yeast plasmid. Twist's price was half that of the nearest competitor (IDT gBlocks) even including shipping. While their synthesized fragments have adapter sequences added to the 5' and 3' ends (you can pay extra to get your product without these), that was not a problem for my application. I received over 1 microgram of DNA per fragment I ordered, which saved me the trouble of having to amplify things by PCR. Sequences were all 100% accurate, at least as I saw when sequencing transformants and the products arrived within the promised time window of 7-9 business days. Overall, very pleased with Twist's gene fragments product!"
Status:
ReviewerMember since: 2021
Organization: Barts and The London School of Medicine and Dentistry, Queen Mary University of London
Plasmid construct and/or synthetic DNA delivered on time and works everytime
Application Area:Antibody Engineering
"Twist is a great company for synthetic DNA for a range of applications. We use them for recombinant antibody engineering, in particular for our GloBody(TM) technology."
Status:
ReviewerMember since: 2022
Organization: Rice University
High quality gene fragment for low cost
Application Area:Molecular Cloning
"High quality gene fragments that are much cheaper compared to competitors. Turn around time was a little bit longer than I would have liked, but also understandable given the volume."
Status:
ReviewerMember since: 2022
Organization: Technical University Kaiserslautern
Twist offers currently the best price for synthetic genes on the planet!
Application Area:Protein expression
"Ordering synthetic genes at Twist is great. Thusfar I ordered gene fragments which we PCRed and cloned into plasmids ourselves. But I will get vectors on board and get the full cloning done soon. Unbeatable prices!"
Status:
ReviewerMember since: 2022
Organization: ASFALIA
Great results; I am happy to be able rely on Twist Bioscience.
Application Area:Analyse promoter/ enhancer sequences
"Twist Bioscience is one of the rare companies synthesizing gene blocks without linkers. This is a very handy tool for very efficient complex cloning. Moreover their prices are quite reasonable as well as the time of production. In addition, they are very reactive and helpful. On one occasion my sequence was missing a few bases and it was replaced quickly. I strongly recommend this company for gene synthesis."
Status:
ReviewerMember since: 2022
Organization: uPetsia
Great result for my experiment with low cost and short waiting time
Application Area:Microbiology
"I requested 3 different fragments. All turns out 100% accurate from 2 colonies of each cloning. The price is reasonable, actually it is about 3-5 times cheaper than other such as Genscript. I highly recommend if you are interested in DNA fragments. I never have chance to request a cloning service since synthesizing of DNA fragment is enough for the gene cloning."
Status:
ReviewerMember since: 2022
Organization: FMP berlin
Fast turnaround time, allows difficult sequences and very well priced
Application Area:Gene fragments
"I ordered six gene fragments on Twist. Many sequences that were rejected by other companies were still possible with Twist. 5/6 sequences succeeded and we were reimbursed for the failed fragment. Sequences arrived within two weeks. Very pleased with Twist so far!"
Status:
ReviewerMember since: 2022
Organization: Targene
Great service. Intuitive website.
Application Area:Synthetic biology
"For the last 3 years I'm placing orders with Twist Bioscience. The experience is always positive. I highly recommend even trying the website since it is very customer friendly. The prices are reasonable and the service is great. You can always consult with customer service or with your local representitive."
Status:
ReviewerMember since: 2022
Organization: Cornell University
One of the best DNA part provider.
Application Area:Recombinant protein production
"Products are delivered in the fastest speed possible. However if there are QC issues, email notifications are provided to inform the user/purchaser."
Status:
ReviewerMember since: 2022
Organization: Chulalongkorn university
I love it alot.
Application Area:Engineering gene in yeast
"I love their website, it's easy to order, just put the sequence, see the price, pay by credit card, waiting the gene/fragment/clone and start the experiments."
Status:
ReviewerMember since: 2022
Organization: LANL
Overall very satisfied
Application Area:Antibody gain of function
"Product arrived within the expected time frame. Communication with Twist scientist and manager was very easy and effective. The products arrived well packaged and exactly like requested. Efficiency of transformation of the gene fragments in yeast was as expected."
Status:
Advanced ReviewerMember since: 2021
Organization: Esperovax
Great service & customer support at a great price, and fast delivery.
Application Area:Yeast expression gene cloning for vaccine development
"We have used Twist to generate many gene blocks we have used in our cloning procedure. They are fast, less costly than competitors, have great QC, and will not send out anything until 100% correct. They had some growing pains when their production was shut down and our orders were delayed but that seems to be resolved. I do wish they would relax some of their constraints and allow more complex sequences to be produced. Others accept those more difficult stretches of DNA and charge more based on complexity."
Status:
Advanced ReviewerMember since: 2019
Organization: University of Chicago
High quality DNA synthesis with fast delivery
Application Area:Synthetic gene construction
"We have found this to be our preferred DNA synthesis service for gene fragments based on the price per base pair, turn around time and quality. The online interface is straightforward and the ability to assess sequence for suitability for their service has been helpful. The customer support is excellent."
Status:
ReviewerMember since: 2021
Organization: UT Southwestern Medical Center
Good prouducts and services.
Application Area:For cloning genes
"The genes ordered were rapidly synthesized and shipped to me. The cost is lower than I thought. The products are good enough for my downstream work. Great experience!"
Status:
ReviewerMember since: 2021
Organization: MD Anderson
Affordable quality gene synthesis
Application Area:Creation of gene fragment or mutant
"The fragment came quickly worked on the first try and were the most economical out there. Highly recommend!"
Status:
ReviewerMember since: 2021
Organization: uc berkeley
Great product! Excellent pricing and fast turnaround!
Application Area:Cloning and expression
"I ordered gene fragments for cloning and expression. They offered the best price and fast turnaround times."
Status:
ReviewerMember since: 2018
Organization: Weill Cornell Medical College
Great customer service
Application Area:Protein interaction studies
"I am a returning customer. Great customer service. There was an issue with adapters halfway through synthesis and the company was quick to remedy the issue. Will be back to order more gene fragments."
Status:
ReviewerMember since: 2021
Organization: AlphaZyme
Great company to work with
Application Area:Protein expression
"The DNA fragment ordering is very easy and inexpensive. We love to use Twist's services for our cloning needs."
Status:
ReviewerMember since: 2018
Organization: Weill Cornell Medical College
Great product, affordable price and fast turnaround.
Application Area:Biochemistry and protein-protein interactions
"We have ordered from Twist in the past but this was our first larger order. Everything about the process was easy. I also enjoy being able to monitor the order progression online. We will gladly order again in the future."
Status:
ReviewerMember since: 2021
Organization: UCSF
Great and reliable results!
Application Area:Gibson reactions and plasmid experiments
"Twist is a really nice vendor. It is easier to order, to make changes and their platform is really friendly. Prices are quite accessible and reasonable. Fragments make it soon and are super reliable when they are sequenced. Highly recommended"
Status:
ReviewerMember since: 2021
Organization: CNETE
Good company and good products!
Application Area:Molecular biology
"The software to optimize and modify the sequence is the best. It's simple and efficent. The delay is good and you can know at which step they are for the synthesis of your DNA. When you have a question, the answer comes pretty fast. The price is also good. Good company and good products!"
Status:
ReviewerMember since: 2021
Organization: Medical University of Innsbruck
Great pricing and great customer care!
Application Area:Codon optimization for protein production
"I had a very good experience. The pricing of gene fragments is the cheapest available. The production encountered some issues but the customer care was great and the delay did not cause problems."
Status:
ReviewerMember since: 2021
Organization: OHSU
Great results and service
Application Area:Synthetic oligos for in vitro transcription assays
"This product was reasonably priced and delivered quickly. We had tried other manufacturers but they were unable to produce our gene fragment due to the complex sequence involved. Twist was able to synthesize these oligos without a problem."
Status:
ReviewerMember since: 2021
Organization: NC State University
Great result and reliable vendor, I'll keep choosing Twist!
Application Area:Express proteins for in vitro assay
"The gene fragments are easy to use to construct a plasmid. It is efficient due to the sequence being correct and reliable. The price is reasonable, and the online service is always in time, and the reps answer my question very fast."
Status:
ReviewerMember since: 2021
Organization: Barts and The London School of Medicine and Dentistry, Queen Mary University of London
Great, reliable service.
Application Area:Recombinant Antibody Research
"Synthetic DNA codon optimised either as fragments or cloned into expression vectors saves time and costs. Works every time."
Status:
ReviewerMember since: 2021
Organization: U of Idaho
Love the price of the fragments!
Application Area:Gene fragments for qPCR standards
"The gene fragments are a great price. I love that I can put in my sequences and hit order. The follow-up emails with one customer rep were fantastic! Thank you for being my science buddy!"
Status:
ReviewerMember since: 2021
Organization: Texas A&M University
Great company! Makes the difficult simple!
Application Area:Protein production, and protein-protein interactions
"I love using Twist Bioscience for their capability to produce difficult strands of DNA, especially my repetitive GC-rich regions. They also have an algorithm that simplifies correcting the DNA into a strand that they can produce. Also, if anything goes wrong they don't charge you and you can even talk to a sales rep to have them handle the case personally."
Status:
ReviewerMember since: 2021
Organization: Orion Corporation
Twist is easy, fast and good value for money.
Application Area:Protein engineering
"Twist eCommerce is very easy to use and ordering a few variants of a gene was very straight forward."
Status:
ReviewerMember since: 2021
Organization: University of Utah
Great price and comparable (or slightly better) service compared to other companies!
Application Area:Cloning
"I ordered 3 gene fragments. The prices were less than half of what the same gene fragments would have costed from another service. I didn't need to create any sequence changes in my gene fragments ordered from Twist while another service said that the same sequences had too many repeats. I got higher amounts of DNA than I would have from a different company - Twist has the option to give you only 1000ng if you just want that much or to get as much as they produce without a cap (I got 1400-1800ng) while another service I use caps theirs at 1000ng for fragments of the same lengths. The turn-around time is supposed to be 9 business days although it took ~11 in my case possibly due to Covid-19 related delays. The customer service is quick and responsive via chat if you have any questions. I felt less guilty about ordering costly gene fragments instead of going the old-school route for simple cloning to save money."
Status:
ReviewerMember since: 2021
Organization: Universitat Bonn
I love these gene fragments!
Application Area:Research in plant pathogen interactions
"The planning and ordering of the gene fragments was easy and without any problems. After 7 days we obtained our strings - it's nearly "clone by phone" just a bit more expensive!"
Status:
ReviewerMember since: 2021
Organization: Lygos, Inc.
Best price for gene fragments
Application Area:Metabolic engineering
"Least expensive option for synthetic gene fragments out there. The product is good quality. Quick turnaround time and excellent customer service."
Status:
ReviewerMember since: 2021
Organization: Medizinische Hochschule Hannover
Best source for gene-blocks, price & service-wise.
Application Area:Molecular biology
"For standard application regarding molecular biology we were looking for relatively quick synthesis, while also not wasting money, since we are reliant on synthesized genes regularly. A lot of genes are still not accessible through sharing-portals like AddGene, plus TWIST Biosciences is a more secure way to get the product in the desired time frame. The price of around 100-200 dollars per gene is okay, however, if there was a discount for bulk-ordering, maybe this would be beneficial sometimes. I ordered a handful of times already. Usually I accidentally make mistakes in the shipping details, therefore im a good benchmark for the service they offer. I cant complain, they fixed all my mistakes via chat, always answering immediately. I almost felt bad at one point. Really friendly and helpful service!"
Status:
ReviewerMember since: 2021
Organization: CRUk CI
Great results.
Application Area:Molecular biology
"I ordered gene blocks (DNA fragments) from twist for Gibson cloning. Website is very easy to use, there is a support and costumer service along each step, if needed. Price is very competitive and most important it was a great success with 100 positive colonies."
Status:
Advanced ReviewerMember since: 2015
Organization: IRB Barcelona
Fast synthesis service, excellent pricing and first 2 clones 100% sequence correct.
Application Area:Gene fragments for protein expression
"Fast synthesis service, excellent pricing and first 2 clones 100% sequence correct by sequencing. We chose fragments because we already have a rapid, standard, ligation independent cloning method in the lab using our own plasmid suite so the fragment went directly into our expression plasmid. Very pleased."
Status:
Advanced ReviewerMember since: 2019
Organization: University of Chicago
Fast production times coupled with a reliable gene fragment.
Application Area:Recombinant technology for in vivo manipulation
"We have ordered multiple gene products from Twist Bioscience and have always had excellent results. The turnaround time is rather quick and often arrives prior to the stated date. Our clones have always produced our expected sequence and have been used successfully throughout our experimental workflow. Customer support is excellent. My preferred service for gene fragment synthesis."
Status:
ReviewerMember since: 2021
Organization: Astrazeneca
A gene synthesis company that confidently provides what you need for your project.
Application Area:Gene synthesis
"Great quality DNA - perfect sequencing reads after cloning is complete. Excellent sales and technical support team."
Synthetic gene fragments are a simple and increasingly popular alternative to PCR-based cloning. Access to synthetic genes has been limited by cost, turnaround time, throughput, and quality. Twist Bioscience gene fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.
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