Gene Fragments by Twist Bioscience

"Quick and cost effective service, in case of questions they generally reply to emails within one day."

"The gene rragments worked really well, The service from Twist Bioscience was excellent. We use Twist all the time now for my gene fragments."

"The interface was easy to use. Twist sent me the ordered product in a reasonable amount of time, and was actually faster than their estimated date. I PCR'd and sequenced one of the gene fragments I ordered due to high complexity, and the gel purified band of the correct size was sequence perfect. The only small issue was that the amount of DNA varied between tubes by around 30-50% of the expected value(some were higher, some were lower), despite asking for normalization on their end. I still had more than enough for golden gate reactions."

"I was really satisfied with my order. First of all, the platform is really easy use. Then, I was amazed on how fast the gene fragments were synthesised. The order arrived 2 days before we expected and given the high quality of the products we build a library that otherwise would have taken months to be ready."

"They offer a very good service, with friendly user interface, clearly stating what is needed to improve on your orders, and offering a variety of options. Price-wise, it could be slightly better as for shorter sequences or a lower amount of gene fragments ordered makes the price per base notably increased."

"Twist is extremely easy to use and provides the lowest cost for gene fragment compared with their competitors. The product quality is always perfect and as advertised. The pricing makes it possible to take on much more ambitious goals and their option to clone the genes for us adds even more possibility for increased impact. We have found that it is rarely cost effective to clone in-house anymore, and when it is, we can often still use gene fragments to speed the process along and give us more options. Their vector onboarding allows us to use our in-house vectors for their clonal gene service, but most of the time their existing vector options are perfect for our applications. The genes take around 3 weeks to arrive, so we plan further in advance and take on more projects during the meantime. Their website and UI is extremely intuitive and easy to work with, unlike many competitor websites. I love working with Twist and I go to them first for any cloning project I have."

"The order platform is very easy to use. The order was quickly placed and started to be managed. The synthetized DNA is correct in sequence even for long fragments. Flanking adaptators allow you to amplify your DNA if need be. Order has arrived on time !"

"The quality of Twist product is very good. I have ordered gene fragments from other vendors in the past but have had high failure rates and longer than published turn around time. Ordering with Twist, I have had much better overall synthesis success rate and the turnaround time is usually very fast. Not to mention, this is the cheapest pricing for gene fragments. I have switched to using Twist for all my gene synthesis needs."

"I have worked in Molecular Biology for over 20 years and have pretty much seen it all in terms of experience with different vendors. I can confidently say that Twist checks ALL the boxes. In our recent interaction, the most important box checked was the high sequence accuracy of the fragments we ordered. With a 94% sequence accuracy rate, we did not have to go back to pick additional colonies for any of our 48 constructs. The ordering process was simple and turnaround times and prices are highly competitive. Fragments are synthesized in the United States so the shipping is faster with no risk of potential customs delays. I have had positive interactions with everyone I’ve communicated with at Twist and the customer service has been great! There was even follow-up to make sure we were satisfied with our order. I’m very appreciative of Twist and the top-notch products, services, and technologies they have to offer!"

"Gene fragments were of high quality. The order process is smooth and easy to navigate. Very reasonable prices and excellent customer service!"

"This allows us to save time and money."

"I ordered gene fragments from Twist to clone a number of point mutants of a gene of interest into a yeast plasmid. Twist's price was half that of the nearest competitor (IDT gBlocks) even including shipping. While their synthesized fragments have adapter sequences added to the 5' and 3' ends (you can pay extra to get your product without these), that was not a problem for my application. I received over 1 microgram of DNA per fragment I ordered, which saved me the trouble of having to amplify things by PCR. Sequences were all 100% accurate, at least as I saw when sequencing transformants and the products arrived within the promised time window of 7-9 business days. Overall, very pleased with Twist's gene fragments product!"

"Twist is a great company for synthetic DNA for a range of applications. We use them for recombinant antibody engineering, in particular for our GloBody(TM) technology."

"High quality gene fragments that are much cheaper compared to competitors. Turn around time was a little bit longer than I would have liked, but also understandable given the volume."

"Ordering synthetic genes at Twist is great. Thusfar I ordered gene fragments which we PCRed and cloned into plasmids ourselves. But I will get vectors on board and get the full cloning done soon. Unbeatable prices!"

"Twist Bioscience is one of the rare companies synthesizing gene blocks without linkers. This is a very handy tool for very efficient complex cloning. Moreover their prices are quite reasonable as well as the time of production. In addition, they are very reactive and helpful. On one occasion my sequence was missing a few bases and it was replaced quickly. I strongly recommend this company for gene synthesis."

"I requested 3 different fragments. All turns out 100% accurate from 2 colonies of each cloning. The price is reasonable, actually it is about 3-5 times cheaper than other such as Genscript. I highly recommend if you are interested in DNA fragments. I never have chance to request a cloning service since synthesizing of DNA fragment is enough for the gene cloning."

"I ordered six gene fragments on Twist. Many sequences that were rejected by other companies were still possible with Twist. 5/6 sequences succeeded and we were reimbursed for the failed fragment. Sequences arrived within two weeks. Very pleased with Twist so far!"

"For the last 3 years I'm placing orders with Twist Bioscience. The experience is always positive. I highly recommend even trying the website since it is very customer friendly. The prices are reasonable and the service is great. You can always consult with customer service or with your local representitive."

"Products are delivered in the fastest speed possible. However if there are QC issues, email notifications are provided to inform the user/purchaser."

"I love their website, it's easy to order, just put the sequence, see the price, pay by credit card, waiting the gene/fragment/clone and start the experiments."

"Product arrived within the expected time frame. Communication with Twist scientist and manager was very easy and effective. The products arrived well packaged and exactly like requested. Efficiency of transformation of the gene fragments in yeast was as expected."

"Good quality and reasonable price and timeline."

"We have used Twist to generate many gene blocks we have used in our cloning procedure. They are fast, less costly than competitors, have great QC, and will not send out anything until 100% correct. They had some growing pains when their production was shut down and our orders were delayed but that seems to be resolved. I do wish they would relax some of their constraints and allow more complex sequences to be produced. Others accept those more difficult stretches of DNA and charge more based on complexity."

"We have found this to be our preferred DNA synthesis service for gene fragments based on the price per base pair, turn around time and quality. The online interface is straightforward and the ability to assess sequence for suitability for their service has been helpful. The customer support is excellent."

"The genes ordered were rapidly synthesized and shipped to me. The cost is lower than I thought. The products are good enough for my downstream work. Great experience!"

"The fragment came quickly worked on the first try and were the most economical out there. Highly recommend!"

"I ordered gene fragments for cloning and expression. They offered the best price and fast turnaround times."

"I am a returning customer. Great customer service. There was an issue with adapters halfway through synthesis and the company was quick to remedy the issue. Will be back to order more gene fragments."

"The DNA fragment ordering is very easy and inexpensive. We love to use Twist's services for our cloning needs."

"We have ordered from Twist in the past but this was our first larger order. Everything about the process was easy. I also enjoy being able to monitor the order progression online. We will gladly order again in the future."

"Easy and fast."

"Twist is a really nice vendor. It is easier to order, to make changes and their platform is really friendly. Prices are quite accessible and reasonable. Fragments make it soon and are super reliable when they are sequenced. Highly recommended"

"The software to optimize and modify the sequence is the best. It's simple and efficent. The delay is good and you can know at which step they are for the synthesis of your DNA. When you have a question, the answer comes pretty fast. The price is also good. Good company and good products!"

"I had a very good experience. The pricing of gene fragments is the cheapest available. The production encountered some issues but the customer care was great and the delay did not cause problems."

"This product was reasonably priced and delivered quickly. We had tried other manufacturers but they were unable to produce our gene fragment due to the complex sequence involved. Twist was able to synthesize these oligos without a problem."

"The gene fragments are easy to use to construct a plasmid. It is efficient due to the sequence being correct and reliable. The price is reasonable, and the online service is always in time, and the reps answer my question very fast."

"Synthetic DNA codon optimised either as fragments or cloned into expression vectors saves time and costs. Works every time."

"The gene fragments are a great price. I love that I can put in my sequences and hit order. The follow-up emails with one customer rep were fantastic! Thank you for being my science buddy!"

"I love using Twist Bioscience for their capability to produce difficult strands of DNA, especially my repetitive GC-rich regions. They also have an algorithm that simplifies correcting the DNA into a strand that they can produce. Also, if anything goes wrong they don't charge you and you can even talk to a sales rep to have them handle the case personally."

"Twist eCommerce is very easy to use and ordering a few variants of a gene was very straight forward."

"I ordered 3 gene fragments. The prices were less than half of what the same gene fragments would have costed from another service. I didn't need to create any sequence changes in my gene fragments ordered from Twist while another service said that the same sequences had too many repeats. I got higher amounts of DNA than I would have from a different company - Twist has the option to give you only 1000ng if you just want that much or to get as much as they produce without a cap (I got 1400-1800ng) while another service I use caps theirs at 1000ng for fragments of the same lengths. The turn-around time is supposed to be 9 business days although it took ~11 in my case possibly due to Covid-19 related delays. The customer service is quick and responsive via chat if you have any questions. I felt less guilty about ordering costly gene fragments instead of going the old-school route for simple cloning to save money."

"The planning and ordering of the gene fragments was easy and without any problems. After 7 days we obtained our strings - it's nearly "clone by phone" just a bit more expensive!"

"Least expensive option for synthetic gene fragments out there. The product is good quality. Quick turnaround time and excellent customer service."

"For standard application regarding molecular biology we were looking for relatively quick synthesis, while also not wasting money, since we are reliant on synthesized genes regularly. A lot of genes are still not accessible through sharing-portals like AddGene, plus TWIST Biosciences is a more secure way to get the product in the desired time frame. The price of around 100-200 dollars per gene is okay, however, if there was a discount for bulk-ordering, maybe this would be beneficial sometimes. I ordered a handful of times already. Usually I accidentally make mistakes in the shipping details, therefore im a good benchmark for the service they offer. I cant complain, they fixed all my mistakes via chat, always answering immediately. I almost felt bad at one point. Really friendly and helpful service!"

"I ordered gene blocks (DNA fragments) from twist for Gibson cloning. Website is very easy to use, there is a support and costumer service along each step, if needed. Price is very competitive and most important it was a great success with 100 positive colonies."

"Fast synthesis service, excellent pricing and first 2 clones 100% sequence correct by sequencing. We chose fragments because we already have a rapid, standard, ligation independent cloning method in the lab using our own plasmid suite so the fragment went directly into our expression plasmid. Very pleased."

"TwistBiosciences never lets me down."

"We have ordered multiple gene products from Twist Bioscience and have always had excellent results. The turnaround time is rather quick and often arrives prior to the stated date. Our clones have always produced our expected sequence and have been used successfully throughout our experimental workflow. Customer support is excellent. My preferred service for gene fragment synthesis."

"Great quality DNA - perfect sequencing reads after cloning is complete. Excellent sales and technical support team."