FastDNA™ Spin Kit for Feces
The FastDNA SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.
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The FastDNA SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.
Host cells, bacteria, fungi, viruses, protists and other cells present in fecal samples are easily lysed within 40 seconds with any FastPrep® instrument.
The FastDNA SPIN Kit contains pre-filled 2 mL Lysing Matrix tubes for the homogenization step as well as all the reagents needed to extract DNA in a purification process eliminating PCR inhibitors.
Application Notes
The Fast DNA® SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Used to isolate PCR-ready genomic DNA from fresh or frozen human and animal stool samples
Optimization of 16S rRNA gut microbiota profiling of extremely low birth weight infants
Infants born prematurely, particularly extremely low birth weight infants (ELBW), have altered gut microbial communities. There is a requirement to optimally characterize microbial profiles in this at-risk cohort, via standardization of methods, particularly for studying the influence of microbiota therapies (e.g. probiotic supplementation) on community profiles and health outcomes. Profiling of faecal samples using the 16S rRNA gene is a cost-efficient method for large-scale clinical studies to gain insights into the gut microbiota and additionally allows characterization of cohorts where sample quantities are compromised. To this end, the bacterial DNA extraction protocol from ELBW infant faeces was optimized by testing three different DNA extraction methods.
Exploring the world of microbiomes – Insights of state-of-the-art solutions for metagenomic approaches
To explore the natural microbial community of any ecosystem by high-resolution molecular approaches, it is extremely important to develop sensitive and reproducible nucleic acid extraction methods that facilitate isolation of microbial DNA and RNA of sufficient purity and quantity.
Proper lysis of microbial cells without damaging their genomes is the first major challenge. Then, as a second step, isolation and extraction must yield high quality nucleic acid for subsequent metagenomic studies.
MP Biomedicals supplies a complete solution from sample grinding, cell lysis to nucleic acid extraction and purification.
In order to offer a ready-to-use, flexible and reliable solution for nucleic acid isolation, MP Biomedicals has developed a portfolio of FastPrep bead beating instruments together with a broad panel of Lysing Matrix tubes to grind and lyse thoroughly any sample type and built up a comprehensive range of organic-based, spin column-based as well as magnetic beads-based extraction kits for the isolation of high-quality DNA and RNA.
Key learning objectives
- Learn how to obtain thorough homogenization and lysis of any microorganism from samples used in microbiome research
- Discover how the FastDNA™ SPIN Kit for Soil from MP Biomedicals has been found to be one of the most suitable kits available for DNA extraction and microbiome sequencing
- Be informed of the new DNA isolation kits developed by MP Biomedicals to generate high yields and achieve rapid isolation of genomic DNA from environmental samples and water samples
- Discover the MPure-32™ Automatic Nucleic Acid Purification instrument designed for high-throughput applications and combined kits specially developed for DNA purification from various samples including feces and environmental samples
Who should attend?
- Scientists involved in metagenomic analysis of human and animal microbiome samples (biomedical research & veterinary research)
- Researchers working in the environmental and agricultural research field (with soil, sediment, water, sludge, compost, rhizosphere, wood, any other plant specimens as well as food samples)
- Scientists investigating bioenergy production processes
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
