Clonal Genes by Twist Bioscience

"My sales rep (Catherine Sutton) is an extremely friendly and helpful person who will do everything to offer you a good quality product at a reasonable price."

"Twist is very easy to use, has a very helpful design tool for plasmid design, the costs are very reasonable and the turnaround time is very good. Highly recommend."

"Here's the deal, Twist is, to my knowledge just, simply getting this work done faster than anyone in the field. I frequently (although not always) only wait two weeks from the time I order until the plasmids hit the lab. This timeframe is extremely helpful and allows us to iterate in our workflow significantly faster. I would not say Twists services are necessarily cheap, but they provide high-quality work you can trust. Which in the end may save you even more time and possibly headaches. They could use some more variation for their in-house e. coli plasmids, but the ones they have are sufficient."

"Twist has made our research very productive. We can design molecules and Twist rapidly produce the recombinant constructs to test our ideas. Todate we have done many projects with Twist and are happy with each and everyone. Wonderful on-line support and follow up."

"Twistbio is one of the best platform for gene synthesis considering time and budget. Only glitch is that it doesn’t accept highly repeated sequences. The team is readily available if any help required with the service."

"Accelerated my work of vector construction, sanger sequencing prove no mutations."

"Twist's clonal products were easy to use. The web portal was simple to find vectors for my desired insert and easy to check for frameshifts. The cost can't be beat either!"

"I have been using Twist cloned gene service for a couple of projects. It was easy to order on Twist's website. The cost and TAT are great. Cloning yourself and sending it for sequencing is more expensive than ordering the construct from Twist. They also NGS verify the clone. Customer service is excellent - You get a knowledgeable service team member within minutes, and they are always happy to help troubleshoot."

"We asked Twist Bioscience to synthesize two expression plasmids carrying a 300 bp cDNA each, coding for small proteins. The original delivery date was 17 working days post-order placement, but as the insert was really short and as we needed data quickly for inclusion in a funding proposal, we asked if it would be possible to get them faster. Not only it was easy to communicate via the chat feature of the website, but people were consistently useful in updating us on progress. Also, our request was taken seriously (no lip service) and, low and behold, we got our plasmids way sooner than anticipated! Great service all around!"

"I recently switch to Twist, I used to buy genes from Genscript; but the price it is really good and the time of delivery is reasonable being myself in Peru. Thanks!"

"They have a variety of plasmids to choose from which saves steps for downstream processing. The software for optimizing the sequence is very intuitive and has a lot of checks built in that help you catch errors in your sequence. I almost forgot to add ATG to my protein! Luckily the software caught it for me. I was able to optimize codons for my model organism which is a great feature."

"The clonal gene service from Twist Bioscience is a great time saver. In addition, it makes it possible to optimize DNA sequence to maximize protein expression in a way that was not previously practical/cost effective. One of the most useful aspects of the service is that you can have your gene cloned into your own custom vector. The service is very cost effective because it covers synthesis, cloning and sequence verification all at once. One area for improvement would be if they would set up an option to allow the synthesized DNA from a single order to be cloned into multiple vectors. This would make the service even more cost effective. One other area for improvement would be if the technology could be more tolerant of sequence complexities, which sometimes make it impossible to synthesize a needed DNA sequence (this is especially true of promoters and UTRs, which can not be codon optimized)"

"We use the Twist clonal gene as part of a biosynthesis gene pathway to edit the genome in plants. The ability to change the codon usage from E Coli to Plant codon usage was very helpful."

"We received a very important tool for our research project. It was quick and cost-efficient."

"It was easy to onboard our vectors and now it is very easy to clone any sequence we want into them. We always get clones from the glycerol stocks and DNA yields are typically quite high. In this most recent run, now sequences failed production and required re-preps (which are always included regardless) so all 55 vectors were delivered before the ETA which was fantastic!"

"We enjoyed the service, the product arrived quickly and we managed to use it without any problems later."

"As a starting point of our European project, we have used Twist Bioscience gene synthesis service to clone > 80 ORFs encoded by African swine fever virus into the pTwist ENTR vector. From this collection, we next took advantage a versatile recombination-based cloning strategy to shuffle the viral ORFs into different vectors and express recombinant proteins using various systems."

"Twist delivered the ordered cloned DNA fragments in good times and all were faithful. The ordering process was a bit frustrating because the design principles for acceptable constructs are not super clear but I eventually managed to find a compromise that passed scoring. I like the wide array of available backbones though I would hope for a broad host range plasmid (pBBR1 or RK2). I guess, I'll have to remedy that by sending them my favorite plasmid next time. I will order again as it saves a lot of time to outsource cloning."

"Clonal genes from Twist are essential to our lab work involving heterologous expression and in vitro enzymatic assays! Twist makes it easy to order, codon optimize the genes, and the prices are unbeatable. The representatives from Twist were also responsive and helpful throughout the ordering process."

"The gene cloning service was easy to use, accurate and production time was very fast, also delivery even if it would arrive to europe. Quality of DNA was high and it was easy to recover after transformation. The value service is very high it has very competitive prices compared with ther options."

"We systematically order our genes from Twist because of: The very simple use of the website, the reactivity, professionalism and kindness of the agents, the speed of gene synthesis and delivery, and the most important, the clonal genes or gene fragments ordered have always been of excellent quality."

"Very efficient and user friendly. Support team give valuable comments and suggestions."

"Gene synthesis at this scale and speed allowed my group to screen quickly several protein targets and identify the front runners for further functional studies. I will use the service routinely and I have recommended it already to colleagues."

"Order genes from them often, first time ordering full clonal service. Products are always excellent (perfect sequences), best value for money (much cheaper than most other services), and decent turnaround time (this time even 3 days earlier than promised!). Gene design (codon optimization, restriction sites etc) is very easy on their platform, full construct with vector somewhat more cumbersome. Plenty of useful add-ons (removal of adapter sequences, glycerol stock preps) at reasonable prices. Can see their service constantly improving. Chat customer support is quick and helpful."

"Twist Bioscience provides a very easy and flexible system to optimize and order your synthetic gene. The customer service is always extremely fast to answer your questions. Price is very competitive. I strongly recommend this service."

"Twist have great customer service. Any time I’ve had an issue they have solved it very quickly. They are also fast and competitively priced."

"We ordered cloned genes for exogenous expression. For proper synthesis and cloning, Twist has an easy-to-use interface that scores your sequence and allows specific changes to be made. They also have a codon optimization tool that allows you to pick specific regions that you don't wish to change, which was really important in our case. Twist also has very reliable customer service that is available for any question. The quality of the plasmids is very good, we received high amounts of them and it's easy to use."

"Really good service and value for money."

"Using the website was unexpectedly easy, my sequences arrived on time and without errors."

"I ordered multiple recombinant human genes codon optimized and with tags. The genes were successfully synthesized and after cloning, I verified through sequencing that there was not a single mutation in any of the genes. Furthermore, in spite of initial trouble during cloning, Twist offered to retry cloning at no additional charge, including a specially long gene."

"Really enabling as a bench scientist to simply order sequence verified plasmids containing our synthetic biology genetic designs and get a tube with plasmid ready for immediate delivery to mammalian cells. Good turnaround - helped me figure out quickly if a new project would be feasible."

"I got three tubes with DNA. resuspended it, cut it and cloned it into my vector."

"I have used Twist Bioscience to purchase gene fragments in the past and recently for clonal gene purchase. The service was quick and easy, although the site seemed a bit complicated at first. However, thanks to forthcoming staff from the online chat I managed my purchase without any issues. Upon arrival products were easy to use, as they contained enclosed instruction on how to proceed (e.g. if product is delivered dry and requires dissolving). In terms of yield, both times the amount was closer to higher than lower amount as suggested upon purchasing, which is also good. Sequencing for both types of products resulted in exact match."

"I enjoyed the web interface for cloning genes and the price of synthetic DNA."

"I ordered the clonal gene for my recombinant protein expression in E.coli. The subcloning was fast in my desired vector and half the price of the other company. I am happy with the service and products and have been a regular customer since 2020. I would definitely order all my clonal genes from Twist bioscience."

"I've been buying Twist genes both clonal and fragments since late 2017. The customer service has been great and products have always been exactly as requested. Shipping times are quick even during a pandemic where most just did not ship, they kept a 17 day turnaround which all things considered is lightning fast. Seamless ordering process and easy to use website with no excess cruft that other synth houses have. Highly recommending their services, especially the ultra fast fragments service of which I have yet to get a faulty clone from!"

"My life completely changed when I discovered Twist Bioscience because I started to buy the plasmids that I need instead of doing them by myself. The results are much faster, reproducible and reliable. The online platform is super easy to use and the team of customer service is available for you at any time in less than one minute of waiting. The service is amazing! Thank you!"

"The synthesized gene in a plasmid is of good quality. I was able to easily clone the gene into different vectors for next steps."

"Twist clonal genes have high quality and ordering interface is very easy to use."

"It is really easy to upload sequences, the people from the "helpdesk" are really efficient and very helpful. The product was delivered as expected. The price is ridiculously low."

"Excellent service at affordable price."

"Gene synthesis antiquates some tedious aspects of experimentation. Twist Biosciences streamlines the process of ordering expression clones. All steps up to and including quote generation are handled through their intuitive web-based ordering interface. The entire process is simple and efficient, and allows you to keep track of multiple projects and to share information with collaborators. Twist follows up after the ordering process. I ordered a set of gene variants cloned into expression vectors and I received them within 2 weeks. The price was half that of competitors ($0.09/base). I will continue to use Twist for future gene synthesis services."

"Easy to order, very intuitive. Automatic quotation price."

"Super easy to order. The whole process is very smoothly designed. Very good time estimates for the whole process. Excellent communication."