Clonal Genes by Twist Bioscience

"The gene cloning service was easy to use, accurate and production time was very fast, also delivery even if it would arrive to europe. Quality of DNA was high and it was easy to recover after transformation. The value service is very high it has very competitive prices compared with ther options."

"We systematically order our genes from Twist because of: The very simple use of the website, the reactivity, professionalism and kindness of the agents, the speed of gene synthesis and delivery, and the most important, the clonal genes or gene fragments ordered have always been of excellent quality."

"Very efficient and user friendly. Support team give valuable comments and suggestions."

"Gene synthesis at this scale and speed allowed my group to screen quickly several protein targets and identify the front runners for further functional studies. I will use the service routinely and I have recommended it already to colleagues."

"Order genes from them often, first time ordering full clonal service. Products are always excellent (perfect sequences), best value for money (much cheaper than most other services), and decent turnaround time (this time even 3 days earlier than promised!). Gene design (codon optimization, restriction sites etc) is very easy on their platform, full construct with vector somewhat more cumbersome. Plenty of useful add-ons (removal of adapter sequences, glycerol stock preps) at reasonable prices. Can see their service constantly improving. Chat customer support is quick and helpful."

"Twist Bioscience provides a very easy and flexible system to optimize and order your synthetic gene. The customer service is always extremely fast to answer your questions. Price is very competitive. I strongly recommend this service."

"Twist have great customer service. Any time I’ve had an issue they have solved it very quickly. They are also fast and competitively priced."

"We ordered cloned genes for exogenous expression. For proper synthesis and cloning, Twist has an easy-to-use interface that scores your sequence and allows specific changes to be made. They also have a codon optimization tool that allows you to pick specific regions that you don't wish to change, which was really important in our case. Twist also has very reliable customer service that is available for any question. The quality of the plasmids is very good, we received high amounts of them and it's easy to use."

"Really good service and value for money."

"Using the website was unexpectedly easy, my sequences arrived on time and without errors."

"I ordered multiple recombinant human genes codon optimized and with tags. The genes were successfully synthesized and after cloning, I verified through sequencing that there was not a single mutation in any of the genes. Furthermore, in spite of initial trouble during cloning, Twist offered to retry cloning at no additional charge, including a specially long gene."

"Really enabling as a bench scientist to simply order sequence verified plasmids containing our synthetic biology genetic designs and get a tube with plasmid ready for immediate delivery to mammalian cells. Good turnaround - helped me figure out quickly if a new project would be feasible."

"I got three tubes with DNA. resuspended it, cut it and cloned it into my vector."

"I have used Twist Bioscience to purchase gene fragments in the past and recently for clonal gene purchase. The service was quick and easy, although the site seemed a bit complicated at first. However, thanks to forthcoming staff from the online chat I managed my purchase without any issues. Upon arrival products were easy to use, as they contained enclosed instruction on how to proceed (e.g. if product is delivered dry and requires dissolving). In terms of yield, both times the amount was closer to higher than lower amount as suggested upon purchasing, which is also good. Sequencing for both types of products resulted in exact match."

"I enjoyed the web interface for cloning genes and the price of synthetic DNA."

"I ordered the clonal gene for my recombinant protein expression in E.coli. The subcloning was fast in my desired vector and half the price of the other company. I am happy with the service and products and have been a regular customer since 2020. I would definitely order all my clonal genes from Twist bioscience."

"I've been buying Twist genes both clonal and fragments since late 2017. The customer service has been great and products have always been exactly as requested. Shipping times are quick even during a pandemic where most just did not ship, they kept a 17 day turnaround which all things considered is lightning fast. Seamless ordering process and easy to use website with no excess cruft that other synth houses have. Highly recommending their services, especially the ultra fast fragments service of which I have yet to get a faulty clone from!"

"My life completely changed when I discovered Twist Bioscience because I started to buy the plasmids that I need instead of doing them by myself. The results are much faster, reproducible and reliable. The online platform is super easy to use and the team of customer service is available for you at any time in less than one minute of waiting. The service is amazing! Thank you!"

"The synthesized gene in a plasmid is of good quality. I was able to easily clone the gene into different vectors for next steps."

"Twist clonal genes have high quality and ordering interface is very easy to use."

"It is really easy to upload sequences, the people from the "helpdesk" are really efficient and very helpful. The product was delivered as expected. The price is ridiculously low."

"Excellent service at affordable price."

"Gene synthesis antiquates some tedious aspects of experimentation. Twist Biosciences streamlines the process of ordering expression clones. All steps up to and including quote generation are handled through their intuitive web-based ordering interface. The entire process is simple and efficient, and allows you to keep track of multiple projects and to share information with collaborators. Twist follows up after the ordering process. I ordered a set of gene variants cloned into expression vectors and I received them within 2 weeks. The price was half that of competitors ($0.09/base). I will continue to use Twist for future gene synthesis services."

"Easy to order, very intuitive. Automatic quotation price."

"Super easy to order. The whole process is very smoothly designed. Very good time estimates for the whole process. Excellent communication."