Clonal Genes by Twist Bioscience

Manufacturer Twist Bioscience
4.9
/
5.0
  |  35 reviews
Twist Bioscience’s platform is capable of synthesizing thousands of genes each day to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high quality DNA synthesis and assembly; qualities that allow Twist to create perfect genes of various lengths and difficulty, NGS-verified and delivered on-time when you need them.


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Clonal Genes
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Average Rating: 4.9
35 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2022

  • Organization: UTEC University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good price & time of delivery
Rating: 5.0

  • Application Area: Gene synthesis

"I recently switch to Twist, I used to buy genes from Genscript; but the price it is really good and the time of delivery is reasonable being myself in Peru. Thanks!"

Review date: 15 Sep 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: University of Michigan



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fast production and delivery times - Friendly customer support
Rating: 5.0

  • Application Area:I'm using the clones I ordered to express and purify a protein that was hard to obtain

"They have a variety of plasmids to choose from which saves steps for downstream processing. The software for optimizing the sequence is very intuitive and has a lot of checks built in that help you catch errors in your sequence. I almost forgot to add ATG to my protein! Luckily the software caught it for me. I was able to optimize codons for my model organism which is a great feature."

Review date: 31 Aug 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: University of Wisconsin-Milwaukee



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great way to save time and money!
Rating: 4.3

  • Application Area:Developmental genetics

"The clonal gene service from Twist Bioscience is a great time saver. In addition, it makes it possible to optimize DNA sequence to maximize protein expression in a way that was not previously practical/cost effective. One of the most useful aspects of the service is that you can have your gene cloned into your own custom vector. The service is very cost effective because it covers synthesis, cloning and sequence verification all at once. One area for improvement would be if they would set up an option to allow the synthesized DNA from a single order to be cloned into multiple vectors. This would make the service even more cost effective. One other area for improvement would be if the technology could be more tolerant of sequence complexities, which sometimes make it impossible to synthesize a needed DNA sequence (this is especially true of promoters and UTRs, which can not be codon optimized)"

Review date: 24 Aug 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Tel Aviv University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results that save time
Rating: 5.0

  • Application Area:Expression genes in plant for genome editing

"We use the Twist clonal gene as part of a biosynthesis gene pathway to edit the genome in plants. The ability to change the codon usage from E Coli to Plant codon usage was very helpful."

Review date: 19 Aug 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: University of South Carolina College of Pharmacy



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Generated constructs work as expected. We received very promising preliminary results
Rating: 5.0

  • Application Area:Gene synthesis for functional analysis of the generated polymorphic variants

"We received a very important tool for our research project. It was quick and cost-efficient."

Review date: 11 Jul 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: BC Cancer



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Convenient, consistent service for high throughput cloning.
Rating: 4.7

  • Application Area:Custom human recombinant antibody production

"It was easy to onboard our vectors and now it is very easy to clone any sequence we want into them. We always get clones from the glycerol stocks and DNA yields are typically quite high. In this most recent run, now sequences failed production and required re-preps (which are always included regardless) so all 55 vectors were delivered before the ETA which was fantastic!"

Review date: 15 Jun 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: ZIS Sweetening Ltd.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great service
Rating: 5.0

  • Application Area:Foodtech

"We enjoyed the service, the product arrived quickly and we managed to use it without any problems later."

Review date: 20 May 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: INRAE



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Easy to design gene constructs, easy to order, cheap, good time of production
Rating: 4.7

  • Application Area:Protein-protein interactions studies

"As a starting point of our European project, we have used Twist Bioscience gene synthesis service to clone > 80 ORFs encoded by African swine fever virus into the pTwist ENTR vector. From this collection, we next took advantage a versatile recombination-based cloning strategy to shuffle the viral ORFs into different vectors and express recombinant proteins using various systems."

Review date: 19 May 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Armata Pharmaceuticals



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Frustrating design tool but an immensely useful service!
Rating: 4.3

  • Application Area:Synthetic biology

"Twist delivered the ordered cloned DNA fragments in good times and all were faithful. The ordering process was a bit frustrating because the design principles for acceptable constructs are not super clear but I eventually managed to find a compromise that passed scoring. I like the wide array of available backbones though I would hope for a broad host range plasmid (pBBR1 or RK2). I guess, I'll have to remedy that by sending them my favorite plasmid next time. I will order again as it saves a lot of time to outsource cloning."

Review date: 10 May 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Scripps Institution of Oceanography



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Twist makes it easy to order, codon optimize the genes, and the prices are unbeatable
Rating: 4.3

  • Application Area:In vitro enzymatic assays

"Clonal genes from Twist are essential to our lab work involving heterologous expression and in vitro enzymatic assays! Twist makes it easy to order, codon optimize the genes, and the prices are unbeatable. The representatives from Twist were also responsive and helpful throughout the ordering process."

Review date: 05 May 2022 | Clonal Genes


  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Instituto de Productos Lacteos de Asturias (IPLA-CSIC)



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results at low prices
Rating: 5.0

  • Application Area:Expression of proteins

"The gene cloning service was easy to use, accurate and production time was very fast, also delivery even if it would arrive to europe. Quality of DNA was high and it was easy to recover after transformation. The value service is very high it has very competitive prices compared with ther options."

Review date: 20 Apr 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: NabGen Technology



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent in every way
Rating: 5.0

  • Application Area:Quick diagnosis

"We systematically order our genes from Twist because of: The very simple use of the website, the reactivity, professionalism and kindness of the agents, the speed of gene synthesis and delivery, and the most important, the clonal genes or gene fragments ordered have always been of excellent quality."

Review date: 19 Apr 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Carl R. Woese Institute for Genomic Biology, UIUC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Support team give valuable comments and suggestion.
Rating: 5.0

  • Application Area:Protein engineering

"Very efficient and user friendly. Support team give valuable comments and suggestions."

Review date: 14 Mar 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Nottingham Trent University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great web interface, quick expert communication and fast synthesis
Rating: 5.0

  • Application Area:Protein expression

"Gene synthesis at this scale and speed allowed my group to screen quickly several protein targets and identify the front runners for further functional studies. I will use the service routinely and I have recommended it already to colleagues."

Review date: 16 Feb 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Massachusetts Institute of Technology



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Excellent and cheap gene production service
Rating: 4.3

  • Application Area:Custom-made gene for enzyme production

"Order genes from them often, first time ordering full clonal service. Products are always excellent (perfect sequences), best value for money (much cheaper than most other services), and decent turnaround time (this time even 3 days earlier than promised!). Gene design (codon optimization, restriction sites etc) is very easy on their platform, full construct with vector somewhat more cumbersome. Plenty of useful add-ons (removal of adapter sequences, glycerol stock preps) at reasonable prices. Can see their service constantly improving. Chat customer support is quick and helpful."

Review date: 23 Jan 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: University of Bari, Department of Biosciences, Biotechnologies and Biopharmaceutics



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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The best gene synthesis service I have ever used!
Rating: 5.0

  • Application Area:Cloning of a codon-optimized gene

"Twist Bioscience provides a very easy and flexible system to optimize and order your synthetic gene. The customer service is always extremely fast to answer your questions. Price is very competitive. I strongly recommend this service."

Review date: 22 Jan 2022 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Birmingham



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent service. Reliable and cost effective.
Rating: 5.0

  • Application Area:Gene synthesis and cloning.

"Twist have great customer service. Any time I’ve had an issue they have solved it very quickly. They are also fast and competitively priced."

Review date: 11 Dec 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Bar-Ilan University



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great service, will absolutely order agian!
Rating: 4.7

  • Application Area:Transfection for exogenous expression of genes of interest

"We ordered cloned genes for exogenous expression. For proper synthesis and cloning, Twist has an easy-to-use interface that scores your sequence and allows specific changes to be made. They also have a codon optimization tool that allows you to pick specific regions that you don't wish to change, which was really important in our case. Twist also has very reliable customer service that is available for any question. The quality of the plasmids is very good, we received high amounts of them and it's easy to use."

Review date: 06 Dec 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Vilnius university



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Really good service and value for money.
Rating: 5.0

  • Application Area:Gene cloning

"Really good service and value for money."

Review date: 03 Dec 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: UT Austin



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Will order again
Rating: 5.0

  • Application Area:Protein engineering

"Using the website was unexpectedly easy, my sequences arrived on time and without errors."

Review date: 18 Oct 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Okinawa Institute of Science and Technology



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Synthetic sequences of very high quality, excellent customer service.
Rating: 5.0

  • Application Area:Recombinant expression of human genes

"I ordered multiple recombinant human genes codon optimized and with tags. The genes were successfully synthesized and after cloning, I verified through sequencing that there was not a single mutation in any of the genes. Furthermore, in spite of initial trouble during cloning, Twist offered to retry cloning at no additional charge, including a specially long gene."

Review date: 27 Sep 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Stanford



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fast, sequence verified, synthetic genes in a plasmid, ready for immediate testing.
Rating: 5.0

  • Application Area:Gene synthesis and cloning

"Really enabling as a bench scientist to simply order sequence verified plasmids containing our synthetic biology genetic designs and get a tube with plasmid ready for immediate delivery to mammalian cells. Good turnaround - helped me figure out quickly if a new project would be feasible."

Review date: 26 Jul 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: ARO, Volcani center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Wonderful results.
Rating: 5.0

  • Application Area:Cloning into a plant expression vector

"I got three tubes with DNA. resuspended it, cut it and cloned it into my vector."

Review date: 11 Jul 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Helsinki



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Overall, great service, thumbs up from me!
Rating: 4.7

  • Application Area:Organelle biogenesis and development

"I have used Twist Bioscience to purchase gene fragments in the past and recently for clonal gene purchase. The service was quick and easy, although the site seemed a bit complicated at first. However, thanks to forthcoming staff from the online chat I managed my purchase without any issues. Upon arrival products were easy to use, as they contained enclosed instruction on how to proceed (e.g. if product is delivered dry and requires dissolving). In terms of yield, both times the amount was closer to higher than lower amount as suggested upon purchasing, which is also good. Sequencing for both types of products resulted in exact match."

Review date: 24 May 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: FALCONBIO



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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The price is great and the web-interface is intuitive.
Rating: 5.0

  • Application Area:Synthetic biology

"I enjoyed the web interface for cloning genes and the price of synthetic DNA."

Review date: 19 May 2021 | Clonal Genes
  • Status:

    Advanced Reviewer

  • Member since: 2021

  • Organization: Philipps University Marburg



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product, cost effective.
Rating: 5.0

  • Application Area:Recombinant protein production

"I ordered the clonal gene for my recombinant protein expression in E.coli. The subcloning was fast in my desired vector and half the price of the other company. I am happy with the service and products and have been a regular customer since 2020. I would definitely order all my clonal genes from Twist bioscience."

Review date: 19 May 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Binomica Labs



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Awesome parts, very affordable even for amateur biologists budget!
Rating: 5.0

  • Application Area:Plant genetic engineering and amateur biology

"I've been buying Twist genes both clonal and fragments since late 2017. The customer service has been great and products have always been exactly as requested. Shipping times are quick even during a pandemic where most just did not ship, they kept a 17 day turnaround which all things considered is lightning fast. Seamless ordering process and easy to use website with no excess cruft that other synth houses have. Highly recommending their services, especially the ultra fast fragments service of which I have yet to get a faulty clone from!"

Review date: 26 Apr 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: USZ



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Simply an amazing service!
Rating: 5.0

  • Application Area:Expression of gene of interest in B cells using plasmids

"My life completely changed when I discovered Twist Bioscience because I started to buy the plasmids that I need instead of doing them by myself. The results are much faster, reproducible and reliable. The online platform is super easy to use and the team of customer service is available for you at any time in less than one minute of waiting. The service is amazing! Thank you!"

Review date: 16 Mar 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: UT Southwestern Medical Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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It's a great service and provided me with great synthesized gene in a plasmid.
Rating: 5.0

  • Application Area:Gene synthesis

"The synthesized gene in a plasmid is of good quality. I was able to easily clone the gene into different vectors for next steps."

Review date: 02 Mar 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Amyris



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Twist gene synthesis greatly improve my productivity.
Rating: 5.0

  • Application Area:Synthetic biology

"Twist clonal genes have high quality and ordering interface is very easy to use."

Review date: 27 Feb 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: CNRS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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The best service on the market.
Rating: 5.0

  • Application Area:Post-translational modifications role on DNA replication

"It is really easy to upload sequences, the people from the "helpdesk" are really efficient and very helpful. The product was delivered as expected. The price is ridiculously low."

Review date: 18 Jan 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Oxford



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Fantastic product and service.
Rating: 4.7

  • Application Area:Cell biology

"Excellent service at affordable price."

Review date: 13 Jan 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: HudsonAlpha Institute for Biotechnology



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Efficient gene synthesis and cloning.
Rating: 5.0

  • Application Area:Gene structure-function analysis

"Gene synthesis antiquates some tedious aspects of experimentation. Twist Biosciences streamlines the process of ordering expression clones. All steps up to and including quote generation are handled through their intuitive web-based ordering interface. The entire process is simple and efficient, and allows you to keep track of multiple projects and to share information with collaborators. Twist follows up after the ordering process. I ordered a set of gene variants cloned into expression vectors and I received them within 2 weeks. The price was half that of competitors ($0.09/base). I will continue to use Twist for future gene synthesis services."

Review date: 04 Jan 2021 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Biochemize



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Definitely my favorite gene supplier.
Rating: 5.0

  • Application Area:Genes for E. coli protein expression

"Easy to order, very intuitive. Automatic quotation price."

Review date: 26 Dec 2020 | Clonal Genes
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of Helsinki



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Very good service.
Rating: 5.0

  • Application Area:Gene synthesis and cloning for protein production

"Super easy to order. The whole process is very smoothly designed. Very good time estimates for the whole process. Excellent communication."

Review date: 23 Dec 2020 | Clonal Genes

Twist Bioscience is transforming gene synthesis, a process at the core of synthetic and molecular biology. Our silicon-based DNA writing platform significantly increases gene synthesis throughput and scalability, while also reducing turnaround time and price per base. Twist’s Clonal Genes offering gives you the flexibility to get the DNA you want, the way you want it. Choose the amount of DNA, and the format you want it in to drive your research. Because every clonal gene is NGS-verified, you can be sure you’re getting the perfect cloned gene every time.

SPECS:

  • 0.3–5.0 kb cloned into a Twist Vectoror your vector of choice
  • 100% accurate NGS-verified gene sequences
  • DNA mass options:
    • 50 ng–2 μg
    • 2–10 μg
    • 10–100 μg
    • 100–1,000 μg
  • Normalization
  • Endotoxin free
  • No order limit