Products & ReviewLife Sciences

Clonal Genes

Twist Bioscience’s platform is capable of synthesizing thousands of genes each day to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high quality DNA synthesis and assembly; qualities that allow Twist to create perfect genes of various lengths and difficulty, NGS-verified and delivered on-time when you need them.

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Twist Bioscience

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Reviews

Average Rating 4.8

|126Scientists have reviewed this product

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Excellent service.

 

Average Rating 5.0

Application Area:

Gene expression plasmids

Easy ordering process, fast synthesis and delivery, and very good technical support when required. The clonal genes also represent competitive pricing too.

Review Date: 17 Mar 2026 | Twist Bioscience

Good experience so far

 

Average Rating 4.0

Application Area:

Research and development

Reliable and efficient gene synthesis service, with options to clone the gene into a vector of your choice. Quick shipping. I have never encountered any issues with the genes purchased from Twist Bioscience.

Review Date: 16 Mar 2026 | Twist Bioscience

High quality, fast speed for production, good price

 

Average Rating 5.0

Application Area:

Cloning gene fragments

As a purchaser and user of clonal genes and gene fragments for a long time, I totally recommend Twist Bioscience for their quality, high speed, easy to use and price. In a week-ten days, samples are ready to ship. And they work fantastically.

Review Date: 14 Mar 2026 | Twist Bioscience

Great service with fast production and delivery

 

Average Rating 5.0

Application Area:

Custom gene sequences

Ordering my sequences was super easy and straightforward, with great user interface and customer service the whole way through. Would highly recommend!

Review Date: 3 Feb 2026 | Twist Bioscience

This product delivers exactly what it says on the tin, fast reliable clonal genes.

 

Average Rating 4.0

Application Area:

Development of novel genetic regulatory systems

We used these products for the assembly od a genetic AND-gate. The rather fast delivery of constructs even with higher genetic complexity significantly cut down on cloning and screening times. Allowing the deconstruction and therefore parallel testing of our genetic constructs. This product delivers exactly what it says on the tin, fast reliable clonal genes.

Review Date: 28 Jan 2026 | Twist Bioscience

The best, really.

 

Average Rating 5.0

Application Area:

Protein production

Tried others but Twist is the best. Fast, convenient and inexpensive.

Review Date: 5 Jan 2026 | Twist Bioscience

A great company providing a great service

 

Average Rating 5.0

Application Area:

Synthetic gene synthesis

I have been using Twist for synthetic gene synthesis for since the pre-Covid era. Always reliable always on time and great customer service.

Review Date: 5 Dec 2025 | Twist Bioscience

Fantastic customer service

 

Average Rating 4.0

Application Area:

Using it in a research lab.

I loved Twist Biosciences customer service. They reply messages promptly and keep things professional. They are always available to help too. They also do reward their customers. I got a free shipping twice in a year and that was awesome. My PI was really happy too!

Review Date: 27 Nov 2025 | Twist Bioscience

Great overall quality

 

Average Rating 5.0

Application Area:

Protein expression

Good order interface. Great service from representatives.

Review Date: 19 Nov 2025 | Twist Bioscience

Fast delivery and helpful customer service

 

Average Rating 5.0

Application Area:

Molecular biology

I ordered 5 sequences as clonal genes, which arrived pretty fast (within a few working days). One of my sequences could not be synthesised into a vector so I got very helpful advice from the customer service and in the end, requested this sequence as linear fragment which worked very well. The customer service responded super fast and with a lot of good information!

Review Date: 26 Sept 2025 | Twist Bioscience

Twist Bioscience is transforming gene synthesis, a process at the core of synthetic and molecular biology. Our silicon-based DNA writing platform significantly increases gene synthesis throughput and scalability, while also reducing turnaround time and price per base. Twist’s Clonal Genes offering gives you the flexibility to get the DNA you want, the way you want it. Choose the amount of DNA, and the format you want it in to drive your research. Because every clonal gene is NGS-verified, you can be sure you’re getting the perfect cloned gene every time.

SPECS:

  • 0.3–5.0 kb cloned into a Twist Vectoror your vector of choice
  • 100% accurate NGS-verified gene sequences
  • DNA mass options:
    • 50 ng–2 μg
    • 2–10 μg
    • 10–100 μg
    • 100–1,000 μg
  • Normalization
  • Endotoxin free
  • No order limit

Application NoteLife Sciences

Multiple focused CRISPR libraries from a single oligo pool

Functional genetic screening using the CRISPR/Cas9 system is a powerful tool for interrogating gene function at scale, but access to and implementation of this technology is constrained by existing pre-defined CRISPR sgRNA libraries.

In this application note, a single Twist Oligo Pool was used in combination with Custom Library Multiplexed Cloning, an easy-to-use bioinformatics and wet-lab workflow, to generate numerous high-quality, custom sgRNA libraries.



Application NoteLife Sciences

Pathway Engineering Through 5 kb Gene Synthesis

A rapid design-build-test cycle is vital to metabolic engineering applications in which researchers re-program microorganisms to produce non-native molecules of interest. To produce these molecules, researchers typically add one gene or an entire pathway of genes into a host organism. These genes encode proteins or enzymes that, when expressed in a microbial host, convert a common substrate into a molecule of interest. Using microbial synthesis as an alternative to chemical synthesis or extraction can yield the same compounds more quickly, at a lower cost, and with fewer environmental impacts.


Application NoteLife Sciences

Clonal Genes: Design. Build. Test.

Twist Bioscience is transforming gene synthesis, a process at the core of synthetic and molecular biology. Our silicon-based DNA writing platform significantly increases gene synthesis throughput and scalability, while also reducing turnaround time and price per base. Think on a new scale in your gene designs, and accelerate your discoveries.








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