Clonal Genes by Twist Bioscience

"Twist pSFFV vectors are our "goto" when we need to clone a DNA repair gene for validation and protein tracking experiments. It is fast and relatively cheap, considering that cloning would also take a long time and require many other steps. I have ordered more than 10 plasmids with inserts in them to date and it has never failed. The return time is about 2 weeks. I am very happy with the service and will definitely always consider it to be a great support for our research."

"Fast, cheap, and a new era for cloning."

"I got the plasmid very quickly (~1.5 weeks after ordering) and they gave me a lot of product (2000 ng). It was the cheapest gene synthesis service I could find ($450 for a 3kb gene in a plasmid). I also got amazing transformation efficiency into TOP10 chemically competent E. coli."

"We received good quality clonal gene which met all the industrial standards . We appreciate the service from Twist sales team as they provided clarifications for all our queries and guided us through every step"

"Twist allows me to plug in my custom gene sequences in an easy to use interface and order them seamlessly cloned into my own custom plasmid (on-boarded previously). This allows me to work with mutations and gene designs that would be very hard to achieve by traditional cloning methods - plus the plasmids arrive already sequence-verified. Genes can be imported either as nucleotide sequence or as AA sequence, with organism-specific codon optimization available, which is helpful for transgenic expression. Price and speed are ok, definitely worth freeing up your lab time from cloning, if your lab can afford it."

"TWIST Biosciences have provided reliable and efficient cloning services for us. We recommend highly to the scientists who need cloning services."

"Order gene fragment from Twist that another company said was not possible to make. Initial attempt did not pass QC but Twist scientist told me the issue and after a redesign successful manufacture"

"The cloned gene in the pTwist plasmid has been produced in a very short time with a high quality The production started before the payment from my University I'm very satisfy because this allows to reduce the time of my research project"

"Fast and effective communication throughout the process. The web tools for ordering were also very good. I am happy with the service and product!"

"Would highly recommend this fast, efficient and cost effective service. Produced 4 genes in under three weeks with no hassle and easy delivery. Beat hands down the UK competitors."

"Twist Bioscience has changed the way we do cloning, which means we will never do ourselves again! The entire experience has really blown our minds; very user friendly platform, prompt insightful help and assistance, fast turnaround time and unparalleled pricing. Using Twist did not just save us money, it saved us a lot of time. Amazing!"

"The cloned plasmids arrived quicker than expected and there was more than enough sample to transform into my strains multiple times. Inserts are also NGS verified before being sent, so I can be sure I am working with the right construct."

"I am 100% satisfied with the gene cloning service that Twist Bioscience offers. Specifically, I commend Shuen Sun and Aparajita Banerjee for their excellent customer service. For example, they responded promptly, addressed my questions within the same day, and helped me troubleshoot getting the product. Many of my colleagues who are experts in molecular cloning and CRISPR-Cas9 mediated editing also recommended Twist!"

"A very easy-to-use site to place an order. Chat staff is competent, polite, friendly, and available to respond in real-time. The ordered material (clonal genes) arrived earlier than expected and works correctly. The amount in nanograms is lower than stated, about half when quantified with Qubit."

"Clonal gene was perfect and without any mistakes. Arrived with some delay due to customs, but with the reasonable price it was still less hassle than cloning it myself."

"It’s reliable, quick, and easy to order. The prices are competitive compared to other companies. And if you have issues, they do respond and try to fix it."

"Twist Bioscience is a leader in gene synthesis, and their service offering is a game changer for synthetic biology. I recently had the opportunity to use Twist Bioscience's gene synthesis service for a project, and I was thoroughly impressed with their technology and customer service. Firstly, Twist Bioscience's technology is truly cutting-edge. Their platform is capable of synthesizing high-quality DNA fragments up to 3,200 base pairs in length, making it one of the most versatile gene synthesis platforms on the market. This allows for the creation of complex synthetic constructs, and their error rate is incredibly low, which is a testament to their technology and quality control. Secondly, their customer service is fantastic. The team at Twist Bioscience is incredibly responsive and helpful, and they provided us with regular updates on the status of our project. They were also able to answer all of our questions and help us troubleshoot any issues that arose during the process. Overall, I would highly recommend Twist Bioscience's gene synthesis service to anyone in need of high-quality, reliable gene synthesis. Their technology is state-of-the-art, and their customer service is top-notch. I will definitely be using their services again in the future."

"This is my second time to order synthetic genes from Twist. Excellent service, really quick synthesis and delivery times and quality gene products delivered on both occasions. I've used > 15 synthetic constructs from Twist now for various purposes (antigen expression, CHIP experiments, protein purification) and all have worked extremely well. Its the end of days for traditional cloning........even for an oldschool molecular biologist like me"

"We frequently buy clonal genes from Twist. We have been really happy with their customer service and the quality of the synthesis. For anything being produced in E. coli it is really simple to get genes made at an affordable price."

"Review for 2.5kb synthetic DNA fragment in plasmid: Chosen for low price and quick turn around. Reps were helpful. The DNA sequence ordered included a section of DNA that some competitors wouldn't synthesise."

"The whole process (from costumer support/implementation to actual acquisition of batches of genes) was very smooth and effective. The costumer support personnel was always available to answer my questions and solve all issues. The products have fair prices and the company met the expectations both in quality of the product and estimated delivery times."

"Ordering synthetic DNA is very easy through the Twist eCommerce, the products arrive quickly and work as they should."

"I've been using Twist Bio for gene synthesis since 2018. Pricing is excellent and the the turnaround time is very reasonable (you can get gene fragments from other suppliers faster but at a higher cost. ) I usually have them clone into a vector, for a very reasonable cost, so I can go directly to expression studies. They have a decent selection of bacterial and mammalian expression vectors as well as cloning vectors. You also have the option to send them a vector if you need something other than what they offer (e.g. insect cell expression)."

"Reasonable price and quick service"

"Design is a breeze, in particular when you supply an amino acid sequence for codon optimization. Generally reasonable expression for all constructs ordered to date. Quick turnaround, and easy to follow progress of the order."

"I am a graduate student working on a project which requires the construction of hundreds of yeast strains, each carrying a plasmid with a different gene inserted in the plasmid. Twist has saved me an enormous amount of work by offering to not only synthesize these genes, but also subcloning them into the custom vector I would like to use, all for a very reasonable price! Based on my experience with their customer service team, it is evident that Twist truly cares about their customers and the quality of their products/services. Would strongly recommend choosing Twist for all your gene synthesis and cloning needs."

"I heard Twist Bioscience from one of my friends. I was trying to clone two new protein constructs for my project, they are quite time-consuming. Then we decided to synthesize them. My friend told me that he usually orders genes from Twist Bioscience. I compared their prices with other companies and found them to be quite reasonable. My friend told me that they are quite reliable, so we decided to order genes from Twist Bioscience. We placed the order before the Christmas holiday and received the products after New Year. It was pretty fast. We are quite satisfied with their service."

"I recently purchased 15 synthetic genes from Twist Bioscience. All genes were designed and codon optimised using the Twist website which was easy and intuitive to use. Genes were ordered just before the Christmas break so I wasn't expecting them to be deliered to the UK from the US until sometime in January, however all genes were delivered a couple of days after Christmas which was an impressive turnaround (I've previously waited up to 3 months with competitors). The quality and yield of all genes was excellent and I've transformed 5 of the synthetic gene clones into E. coli so far with ease. Pricing from Twist was exceptional, I paid less for these 15 genes than I have previously paid for 6 genes from a competitor. Highly recommend Twist"

"My sales rep (Catherine Sutton) is an extremely friendly and helpful person who will do everything to offer you a good quality product at a reasonable price."

"Twist is very easy to use, has a very helpful design tool for plasmid design, the costs are very reasonable and the turnaround time is very good. Highly recommend."

"Here's the deal, Twist is, to my knowledge just, simply getting this work done faster than anyone in the field. I frequently (although not always) only wait two weeks from the time I order until the plasmids hit the lab. This timeframe is extremely helpful and allows us to iterate in our workflow significantly faster. I would not say Twists services are necessarily cheap, but they provide high-quality work you can trust. Which in the end may save you even more time and possibly headaches. They could use some more variation for their in-house e. coli plasmids, but the ones they have are sufficient."

"Twist has made our research very productive. We can design molecules and Twist rapidly produce the recombinant constructs to test our ideas. Todate we have done many projects with Twist and are happy with each and everyone. Wonderful on-line support and follow up."

"Twistbio is one of the best platform for gene synthesis considering time and budget. Only glitch is that it doesn’t accept highly repeated sequences. The team is readily available if any help required with the service."

"Accelerated my work of vector construction, sanger sequencing prove no mutations."

"Twist's clonal products were easy to use. The web portal was simple to find vectors for my desired insert and easy to check for frameshifts. The cost can't be beat either!"

"I have been using Twist cloned gene service for a couple of projects. It was easy to order on Twist's website. The cost and TAT are great. Cloning yourself and sending it for sequencing is more expensive than ordering the construct from Twist. They also NGS verify the clone. Customer service is excellent - You get a knowledgeable service team member within minutes, and they are always happy to help troubleshoot."

"We asked Twist Bioscience to synthesize two expression plasmids carrying a 300 bp cDNA each, coding for small proteins. The original delivery date was 17 working days post-order placement, but as the insert was really short and as we needed data quickly for inclusion in a funding proposal, we asked if it would be possible to get them faster. Not only it was easy to communicate via the chat feature of the website, but people were consistently useful in updating us on progress. Also, our request was taken seriously (no lip service) and, low and behold, we got our plasmids way sooner than anticipated! Great service all around!"

"I recently switch to Twist, I used to buy genes from Genscript; but the price it is really good and the time of delivery is reasonable being myself in Peru. Thanks!"

"They have a variety of plasmids to choose from which saves steps for downstream processing. The software for optimizing the sequence is very intuitive and has a lot of checks built in that help you catch errors in your sequence. I almost forgot to add ATG to my protein! Luckily the software caught it for me. I was able to optimize codons for my model organism which is a great feature."

"The clonal gene service from Twist Bioscience is a great time saver. In addition, it makes it possible to optimize DNA sequence to maximize protein expression in a way that was not previously practical/cost effective. One of the most useful aspects of the service is that you can have your gene cloned into your own custom vector. The service is very cost effective because it covers synthesis, cloning and sequence verification all at once. One area for improvement would be if they would set up an option to allow the synthesized DNA from a single order to be cloned into multiple vectors. This would make the service even more cost effective. One other area for improvement would be if the technology could be more tolerant of sequence complexities, which sometimes make it impossible to synthesize a needed DNA sequence (this is especially true of promoters and UTRs, which can not be codon optimized)"

"We use the Twist clonal gene as part of a biosynthesis gene pathway to edit the genome in plants. The ability to change the codon usage from E Coli to Plant codon usage was very helpful."

"We received a very important tool for our research project. It was quick and cost-efficient."

"It was easy to onboard our vectors and now it is very easy to clone any sequence we want into them. We always get clones from the glycerol stocks and DNA yields are typically quite high. In this most recent run, now sequences failed production and required re-preps (which are always included regardless) so all 55 vectors were delivered before the ETA which was fantastic!"

"We enjoyed the service, the product arrived quickly and we managed to use it without any problems later."

"As a starting point of our European project, we have used Twist Bioscience gene synthesis service to clone > 80 ORFs encoded by African swine fever virus into the pTwist ENTR vector. From this collection, we next took advantage a versatile recombination-based cloning strategy to shuffle the viral ORFs into different vectors and express recombinant proteins using various systems."

"Twist delivered the ordered cloned DNA fragments in good times and all were faithful. The ordering process was a bit frustrating because the design principles for acceptable constructs are not super clear but I eventually managed to find a compromise that passed scoring. I like the wide array of available backbones though I would hope for a broad host range plasmid (pBBR1 or RK2). I guess, I'll have to remedy that by sending them my favorite plasmid next time. I will order again as it saves a lot of time to outsource cloning."

"Clonal genes from Twist are essential to our lab work involving heterologous expression and in vitro enzymatic assays! Twist makes it easy to order, codon optimize the genes, and the prices are unbeatable. The representatives from Twist were also responsive and helpful throughout the ordering process."

"The gene cloning service was easy to use, accurate and production time was very fast, also delivery even if it would arrive to europe. Quality of DNA was high and it was easy to recover after transformation. The value service is very high it has very competitive prices compared with ther options."

"We systematically order our genes from Twist because of: The very simple use of the website, the reactivity, professionalism and kindness of the agents, the speed of gene synthesis and delivery, and the most important, the clonal genes or gene fragments ordered have always been of excellent quality."

"Very efficient and user friendly. Support team give valuable comments and suggestions."

"Gene synthesis at this scale and speed allowed my group to screen quickly several protein targets and identify the front runners for further functional studies. I will use the service routinely and I have recommended it already to colleagues."

"Order genes from them often, first time ordering full clonal service. Products are always excellent (perfect sequences), best value for money (much cheaper than most other services), and decent turnaround time (this time even 3 days earlier than promised!). Gene design (codon optimization, restriction sites etc) is very easy on their platform, full construct with vector somewhat more cumbersome. Plenty of useful add-ons (removal of adapter sequences, glycerol stock preps) at reasonable prices. Can see their service constantly improving. Chat customer support is quick and helpful."

"Twist Bioscience provides a very easy and flexible system to optimize and order your synthetic gene. The customer service is always extremely fast to answer your questions. Price is very competitive. I strongly recommend this service."

"Twist have great customer service. Any time I’ve had an issue they have solved it very quickly. They are also fast and competitively priced."

"We ordered cloned genes for exogenous expression. For proper synthesis and cloning, Twist has an easy-to-use interface that scores your sequence and allows specific changes to be made. They also have a codon optimization tool that allows you to pick specific regions that you don't wish to change, which was really important in our case. Twist also has very reliable customer service that is available for any question. The quality of the plasmids is very good, we received high amounts of them and it's easy to use."

"Really good service and value for money."

"Using the website was unexpectedly easy, my sequences arrived on time and without errors."

"I ordered multiple recombinant human genes codon optimized and with tags. The genes were successfully synthesized and after cloning, I verified through sequencing that there was not a single mutation in any of the genes. Furthermore, in spite of initial trouble during cloning, Twist offered to retry cloning at no additional charge, including a specially long gene."

"Really enabling as a bench scientist to simply order sequence verified plasmids containing our synthetic biology genetic designs and get a tube with plasmid ready for immediate delivery to mammalian cells. Good turnaround - helped me figure out quickly if a new project would be feasible."

"I got three tubes with DNA. resuspended it, cut it and cloned it into my vector."

"I have used Twist Bioscience to purchase gene fragments in the past and recently for clonal gene purchase. The service was quick and easy, although the site seemed a bit complicated at first. However, thanks to forthcoming staff from the online chat I managed my purchase without any issues. Upon arrival products were easy to use, as they contained enclosed instruction on how to proceed (e.g. if product is delivered dry and requires dissolving). In terms of yield, both times the amount was closer to higher than lower amount as suggested upon purchasing, which is also good. Sequencing for both types of products resulted in exact match."

"I enjoyed the web interface for cloning genes and the price of synthetic DNA."

"I ordered the clonal gene for my recombinant protein expression in E.coli. The subcloning was fast in my desired vector and half the price of the other company. I am happy with the service and products and have been a regular customer since 2020. I would definitely order all my clonal genes from Twist bioscience."

"I've been buying Twist genes both clonal and fragments since late 2017. The customer service has been great and products have always been exactly as requested. Shipping times are quick even during a pandemic where most just did not ship, they kept a 17 day turnaround which all things considered is lightning fast. Seamless ordering process and easy to use website with no excess cruft that other synth houses have. Highly recommending their services, especially the ultra fast fragments service of which I have yet to get a faulty clone from!"

"My life completely changed when I discovered Twist Bioscience because I started to buy the plasmids that I need instead of doing them by myself. The results are much faster, reproducible and reliable. The online platform is super easy to use and the team of customer service is available for you at any time in less than one minute of waiting. The service is amazing! Thank you!"

"The synthesized gene in a plasmid is of good quality. I was able to easily clone the gene into different vectors for next steps."

"Twist clonal genes have high quality and ordering interface is very easy to use."

"It is really easy to upload sequences, the people from the "helpdesk" are really efficient and very helpful. The product was delivered as expected. The price is ridiculously low."

"Excellent service at affordable price."

"Gene synthesis antiquates some tedious aspects of experimentation. Twist Biosciences streamlines the process of ordering expression clones. All steps up to and including quote generation are handled through their intuitive web-based ordering interface. The entire process is simple and efficient, and allows you to keep track of multiple projects and to share information with collaborators. Twist follows up after the ordering process. I ordered a set of gene variants cloned into expression vectors and I received them within 2 weeks. The price was half that of competitors ($0.09/base). I will continue to use Twist for future gene synthesis services."

"Easy to order, very intuitive. Automatic quotation price."

"Super easy to order. The whole process is very smoothly designed. Very good time estimates for the whole process. Excellent communication."