CellTiter-Glo® Luminescent Cell Viability Assay by Promega Corp.

Manufacturer Promega Corp.
4.7
/
5.0
  |  17 reviews
The CellTiter-Glo® Luminescent Cell Viability Assay(a,b) is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® Reagent) directly to ... Read more


CellTiter-Glo® Luminescent Cell Viability Assay by Promega Corp. product image
CellTiter-Glo® Luminescent Cell Viability Assay
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Average Rating: 4.7
17 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2006

  • Organization: TCU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Highly recommended!
Rating: 4.7

  • Application Area: Analysis of cell viability upon drug exposure

"A sensitive viability assay subject to fewest possible chemical interferences from drug carrier/matrices."

Review date: 27 Oct 2019 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Miami



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Consitently good results.
Rating: 5.0

  • Application Area:Cell viability

"Products works reliably every time with little batch to batch variation."

Review date: 26 Oct 2019 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Petra Pharma



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent product, excellent results.
Rating: 5.0

  • Application Area:Test cell viability after treatment with compounds

"Our laboratory regularly uses CellTitier-Glo and achieves very consistent, reproducible results which are essential to our workflow. It is the industry standard for good reason, and will continue to function as a major assay within our lab."

Review date: 26 Oct 2019 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Genentech



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great product, consistent results on batch by batch.
Rating: 4.7

  • Application Area:Investigate cell survival by compound dosing

"Easy to use. Consistent and reliable results. High throughput is possible."

Review date: 25 Oct 2019 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: NCI/NIH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Very good product.
Rating: 5.0

  • Application Area:Screening of anti-tumor small molecules by proliferation assay.

"Very consistent result and less variation in experimental replicates."

Review date: 25 Oct 2019 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: GSK



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Simple protocol, reliable results.
Rating: 4.0

  • Application Area:Viral titre

"Has allowed more robust and quantitative TCID50 titration of HRV, compared to crystal violet staining of monolayers."

Review date: 27 Jun 2019 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Edinburgh University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great kit to check how your cells are doing!
Rating: 5.0

  • Application Area:Viabilty in 2D cultures of stem cell derived hepatocytes

"Easy to use, robust and reproducible results with scalability to high throughput culture systems."

Review date: 27 Jun 2019 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: King's College London



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
High quality, reproducible results.
Rating: 5.0

  • Application Area:Measuring IC50 of compounds

"Very easy to use and gives high quality, reproducible results."

Review date: 27 Jun 2019 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Merck & Co.



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Great results with very large assay window
Rating: 4.3

  • Application Area:Detecting activity and viability of small molecules.

"It is very effective where we can read the activity of cpds and same time get therapeutics window and determine toxicity assay"

Review date: 04 Feb 2019 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Expert Reviewer

  • Member since: 2015

  • Organization: SBP Medical Discovery Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Good reagent for cytotoxicity assay.
Rating: 5.0

  • Application Area:Cytotoxicity

"We use Cell Titer-Glo for cytotoxicity study. Very easy to use, the reagent is stable for more than a year at -20C after reconstitution."

Review date: 27 Jul 2018 | CellTiter-Glo® Luminescent Cell Viability Assay


  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Medimmune



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great Results!! Quick and Reproducible Assays. Ease to use.
Rating: 5.0

  • Application Area:Cell Cytotoxicity Assay

"This is an excellent product that is quick and easy to use, with Luminometer or plate reader which requires fewer steps. Cell washing, removal of medium and multiple pipetting steps are not required. Assay is reproducible and readings are very stable.The product delivery time is fast usually 1-3 days. The technical team is prompt in answering queries and assist in any protocol optimization. The sales team obtain feedback timely and familiarize themselves with new products."

Review date: 22 May 2018 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: The Institute of Cancer Research



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    2 out of 5
Highly recommended.
Rating: 3.7

  • Application Area:Cell viability

"Robust, easy to use, quick protocol, consistent result."

Review date: 01 Mar 2018 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: SPANISH NATIONAL CANCER RESEARCH CENTER



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Rating: 3.7

  • Application Area:Cell Biology

"Quick and easy to use. Low variation between experiments."

Review date: 28 May 2012 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: ST. JUDE CHILDREN'S RES. HOSP.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7
"Excellent, sensitive reagent. Luciferase based. Very high Z values in HTS. More expensive than other reagents but well worth it for ease of use and quality of data."

Review date: 11 Jul 2011 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Queen's University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

Review date: 12 May 2007 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Boehringer Ingelheim



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"A great reagent for running kinase/ATPase assays."

Review date: 04 May 2007 | CellTiter-Glo® Luminescent Cell Viability Assay
  • Status:

    Reviewer

  • Member since: 2006

  • Organization: Southern Research



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

Review date: 08 Jan 2007 | CellTiter-Glo® Luminescent Cell Viability Assay
The CellTiter-Glo® Luminescent Cell Viability Assay(a,b) is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required. The system detects as few as 15 cells/well in a 384-well format in 10 minutes after adding reagent and mixing.

The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® Assay generates a "glow-type" luminescent signal, which has a half-life generally greater than five hours, depending on cell type and medium used. The extended half-life eliminates the need to use reagent injectors and provides flexibility for continuous or batch mode processing of multiple plates. The unique homogeneous format avoids errors that may be introduced by other ATP measurement methods that require multiple steps.