A Homogenous Bioluminescent System for Measuring GTPase, GAP and GEF Activities

  This ready-to-use multimode detection system includes: high-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities.   The GloMax® Discover System is a high-performance multimode detection instrument developed with Promega reagent chemistries to provide a simple means of detecting advanced chemistries. This instrument provides researchers superior luminescence sensitivity and dynamic range for both strong and weak bioluminescence experimental samples, as well as a seamless integration with Promega’s industry leading bioluminescent assays. GloMax® Discover also provides flexible use of filters for fluorescence intensity, BRET, FRET, filtered luminescence and UV-visible absorbance measurements. The instrument is operated by an integrated Tablet PC, which provides quick and easy navigation through the control options. Exporting your results is made seamless with a variety of options, including exporting to your local data network. The GloMax® Discover software provides the required technical elements of a 21 CFR Part 11-compliant system to be used with the appropriate laboratory workflow. GloMax® Discover System Features: Integrated with Promega Assays - Developed and optimized with Promega cell and gene reporter assays for seamless workflow; you will be up and running faster. Easy-to-Use - Simple Tablet PC touch screen navigation with full PC capabilities and a state-of-the-art graphical user interface. Superior Performance - Broader dynamic range, better sensitivity and lower well-to-well cross talk for more usable data from your experiment. Cost-Effective - Spend less time optimizing. Connected to Your Workflow - Use as a standalone instrument or integrate into your high-throughput automated workflow; export data to your laboratory network; enable 21 CFR, Part 11 electronic signature compliance. Applications: Integration with Promega reagent chemistries for detection of fluorescence intensity, BRET, FRET, filtered luminescence and UV-visible absorbance assays. Integration into low- and medium-throughput automation workflows. Optimization with Promega cell and gene reporter assays.

The CellTiter-Glo® Luminescent Cell Viability Assay(a,b) is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required. The system detects as few as 15 cells/well in a 384-well format in 10 minutes after adding reagent and mixing. The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® Assay generates a "glow-type" luminescent signal, which has a half-life generally greater than five hours, depending on cell type and medium used. The extended half-life eliminates the need to use reagent injectors and provides flexibility for continuous or batch mode processing of multiple plates. The unique homogeneous format avoids errors that may be introduced by other ATP measurement methods that require multiple steps.