Xdrop®
Xdrop® is a double-emulsion droplet generator capable of encapsulating living cells, organelles, or DNA fragments, along with assay chemistry, within highly stable, picoliter-volume, droplets. Xdrop® enables high-throughput functional analysis in a single-cell format.
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The Xdrop® platform is a microfluidic droplet system that enables high-throughput single-cell analysis and genomic applications. The instrument encapsulates cells, DNA, or particles into individual double-emulsion droplets, enabling subsequent analysis and sorting on flow cytometers. The instrument may be used with a variety of sample types, including microbial cells, mammalian cells and DNA samples.
Key features:
- Versatile droplets: compatible with incubators, flow cytometers, cell sorters and thermal cyclers
- Reveals population heterogeneity: single-cell format assays
- Accessible to every lab: user-friendly instrument requiring minimal training
Applications:
- Immunotherapy
- cell line development
- screening; genomics
- CRISPR edits
Robust characterization of heterogeneity in CAR T cell cytotoxicity
The detection and analysis of single cells are critical in advancing CAR T cell therapy and understanding its mechanisms of action, such as cytotoxicity. Traditional bulk assays fall short of revealing cellular heterogeneity, which limits their effectiveness in evaluating therapeutic potency. In this application note, the Xdrop single-cell assay from Samplix addresses this challenge by providing high-resolution insights into CAR T cell-mediated cytotoxicity. With the ability to quantify granzyme B secretion and cell-killing activity at the single-cell level, Xdrop offers precise characterization of CAR T cells' functional diversity, enabling automated and detailed analysis of immune cell therapies.
Revealing granzyme B secretion and cell killing dynamics
Traditional bulk assays for cell characterization offer limited insights into cell population heterogeneity, crucial for developing effective immunotherapies. Samplix presents a study that introduces a novel method for single-cell analysis of granzyme B (GzmB) secretion and cell killing dynamics using the Xdrop® DE50 droplet-based system. The study aims to address this gap by co-encapsulating natural killer (NK) cells and their target K562 cells in double-emulsion droplets, enabling simultaneous detection of GzmB activity and cell death. This method provides detailed insights into the functional heterogeneity of cell populations, confirming the established role of GzmB in apoptosis and highlighting the Xdrop system's potential in advancing immune cell therapy development.
