SelectScience
Join Free
ResourceLife Sciences

Revealing granzyme B secretion and cell killing dynamics

Samplix
21 May 2024

Traditional bulk assays for cell characterization offer limited insights into cell population heterogeneity, crucial for developing effective immunotherapies. Samplix presents a study that introduces a novel method for single-cell analysis of granzyme B (GzmB) secretion and cell killing dynamics using the Xdrop® DE50 droplet-based system. The study aims to address this gap by co-encapsulating natural killer (NK) cells and their target K562 cells in double-emulsion droplets, enabling simultaneous detection of GzmB activity and cell death. This method provides detailed insights into the functional heterogeneity of cell populations, confirming the established role of GzmB in apoptosis and highlighting the Xdrop system's potential in advancing immune cell therapy development.

Related products

Request Quote for All Products

Xdrop®

Samplix

Xdrop® is a double-emulsion droplet generator capable of encapsulating living cells, organelles, or DNA fragments, along with assay chemistry, within highly stable, picoliter-volume, droplets.  Xdrop® enables high-throughput functional analysis in a single-cell format. 

(0)

Links

Samplix

Tags

Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Single Cell AnalysisSingle-cell analysis involves studying individual cells to gain insights into their behavior, gene expression, and function. This approach is valuable in cancer research, stem cell biology, and immunology. Explore single-cell analysis products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.ImmunotherapyImmunotherapy is a treatment approach that utilizes the body�s immune system to fight diseases, especially cancer. It includes therapies such as monoclonal antibodies, checkpoint inhibitors, and CAR-T cell therapy. Immunotherapy is a rapidly growing area of research in oncology and autoimmune diseases. Browse our peer-reviewed product directory to find the best immunotherapy tools, compare products, check reviews, and get pricing directly from manufacturers.

Related resources