Transcreener® ADP² Kinase Assays
The Transcreener® ADP² Kinase Assay Kit universally detects ADP produced by any kinase or ATPase using a direct mix‑and‑read format, eliminating coupling steps. Available in FP, FI, and TR‑FRET formats with far‑red tracers to minimize interference, it's HTS-ready across 96‑, 384‑, and 1536‑well plates with robust Z' ≥ 0.7 performance.
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Excellent blend of simplicity, economics & good science. Excellent technical support.
The ADP2 assay is a great choice for measuring activity for any ADP-generating enzyme
The ADP2 assay is a great choice for measuring activity for any ADP-generating enzyme in both real time and endpoint formats. The universal detection system has been very useful for more challenging targets.
Review Date: 31 Jul 2022 | BellBrook Labs
Extremely versatile
Kinetic profiling
The ADP2 assay is a great choice for measuring activity for any ADP-generating enzyme in both real time and endpoint formats. The universal detection system has been very useful for more challenging targets in our lab.
Review Date: 23 Jun 2016 | BellBrook Labs
Excellent blend of simplicity, economics & good science. Excellent technical support.
Review Date: 15 Jun 2011 | BellBrook Labs
The Transcreener® ADP² Kinase Assay is a universal, mix-and-read HTS assay that directly measures ADP, the conserved product of kinase and ATPase reactions. Designed to eliminate complexity and reduce assay interference, it provides a robust, flexible solution for drug discovery and inhibitor screening.
Key Features & Benefits
- Universal Detection – Measures ADP from any kinase or ATPase, eliminating the need for multiple assay platforms.
- Direct Mix-and-Read Format – No coupling enzymes or secondary reactions, minimizing false positives and simplifying workflows.
- Multiple Readout Options – Available in Fluorescence Polarization (FP), Fluorescence Intensity (FI), and TR-FRET formats.
- Far-Red Tracers – Reduce compound interference for cleaner, more reliable data.
- HTS Compatible – Validated for 96-, 384-, and 1536-well plates, with Z’ ≥ 0.7 for reproducibility in screening campaigns.
- Automation Ready – Homogeneous format and stable reagents streamline integration into automated screening environments.
Applications
- High-throughput screening (HTS) of kinase inhibitors
- Mechanism of action and SAR studies
- Profiling ATPases and other nucleotide-dependent enzymes
- Hit-to-lead triage with reduced assay artifacts
