Determination of Km for ATP and Kemptide with Protein Kinase A Enzyme using Transcreener® ADP2 FP Assay
3 Sept 2015Transcreener® is a universal, high throughput biochemical assay based on detection of nucleotides, which are formed by thousands of cellular enzymes — many of which catalyze the covalent regulatory reactions that are central to cell signaling and are high value targets in drug discovery. This application note describes the use of the Transcreener® assay in the determination of Km for ATP and Kemptide with Protein Kinase A Enzyme.
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Transcreener® ADP² Kinase Assays
BellBrook LabsThe Transcreener® ADP² Kinase Assay Kit universally detects ADP produced by any kinase or ATPase using a direct mix‑and‑read format, eliminating coupling steps. Available in FP, FI, and TR‑FRET formats with far‑red tracers to minimize interference, it's HTS-ready across 96‑, 384‑, and 1536‑well plates with robust Z' ≥ 0.7 performance.