3D InSight™ C57BL/6 Mouse Liver Microtissues

This resource describes the handling, maintenance, and transfer of 3D InSight™ microtissues in Akura™ 96 Spheroid Microplates. It outlines essential steps including handling upon arrival, medium exchange, and microscopic analysis to ensure reproducible and reliable in vitro results.

The protocol highlights best practices for preserving microtissue integrity, including controlled pipetting techniques and semi-automated workflows. These approaches support consistent long-term culture and downstream applications such as drug testing, imaging, and biochemical assays.

Resource details:

• Resource type: Application note
• Page count: 18
• Read time: 27 mins

This validation report from InSphero evaluates the performance of 3D InSight™ C57BL/6 mouse liver microtissues, confirming consistent morphology, viability, and functionality over a 28-day culture period. The co-culture model, composed of primary hepatocytes and non-parenchymal cells, demonstrates stable tissue structure and reproducible behavior across experimental conditions.

Functional testing, including albumin secretion, cytochrome P450 (CYP) enzyme activity, and drug-induced liver injury (DILI) assays, supports the model’s suitability for safety assessment and preclinical research. The results confirm that the validated cell lot meets performance criteria for use in liver toxicity studies and related applications.

Resource details:

• Resource type: Application note
• Page count: 21
• Read time: 34 mins

Explore 3D InSight™ species-specific liver microtissues – assay-ready 3D in vitro liver models designed to provide physiologically relevant and reproducible results for laboratory studies. The microtissues consist of co-cultured primary hepatocytes and non-parenchymal cells, enabling more accurate simulation of liver function compared to traditional 2D cultures.

Delivered in a standardized microplate format, the system supports high-throughput and automation-ready workflows, with applications including drug safety, hepatotoxicity assessment, and disease modeling. Discover how the models maintain metabolic activity and functionality for extended culture periods, supporting long-term studies and consistent experimental outcomes.

Resource details:

• Resource type: Application note
• Page count: 21
• Read time: 34 mins

This flyer highlights the applications of 3D InSight™ Liver Microtissues, assay-ready 3D liver spheroid models designed for drug discovery and translational research. Discover how the platform enables researchers to generate human-relevant data across a wide range of applications, including safety testing, pharmacokinetics, and disease modelling.

The Microtissues support advanced workflows such as drug-induced liver injury (DILI) prediction, mechanistic toxicology, antiviral research, and multi-organ studies. By providing standardized, ready-to-use models, the system helps streamline experimental workflows and improve reproducibility in preclinical research.

Resource details:

• Resource type: Scientific poster
• Page count: 2
• Read time: 3 mins

This whitepaper explores the use of cross-species 3D liver microtissues to improve prediction of drug-induced liver injury (DILI) during preclinical development. Using a case study, the work demonstrates how advanced in vitro models can reveal species-specific mechanisms of toxicity that are not captured using traditional animal models or short-term cytotoxicity assays.

By combining long-term exposure studies with functional and transcriptomic analysis, the models enable identification of complex toxicity pathways and support more accurate human risk assessment. The findings highlight the value of integrating 3D in vitro liver systems early in drug discovery workflows to reduce late-stage attrition and improve translational outcomes.

Resource details:

• Resource type: Whitepaper
• Page count: 12
• Read time: 20 mins

InSphero’s 3D InSight™ Microtissues are assay-ready 3D spheroid models designed to accelerate drug discovery workflows. In this video, discover how these physiologically relevant microtissues integrate directly into laboratory processes, enabling researchers to generate consistent and human-relevant data from early-stage studies.