Transcreener® ADP2 TR-FRET Red Assay: Homogeneous ADP Detection
19 Jun 2016In this study the utility of ADP detection as a means to screen inhibitor potency using a far-red, time resolved Förster resonance energy transfer (TR-FRET) format is described. This assay uses the widely adopted TR-FRET technique to take advantage of low compound interference common to this method and owed to its time-gated nature as signal is measured after most background fluorescence has subsided. This assay has proven both sensitive and robust with Z´>0.7 at low substrate conversion levels enabling enzymology during initial velocity conditions and is an important addition to the HTS toolkit.
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Transcreener® ADP² Kinase Assays
BellBrook LabsThe Transcreener® ADP² Kinase Assay Kit universally detects ADP produced by any kinase or ATPase using a direct mix‑and‑read format, eliminating coupling steps. Available in FP, FI, and TR‑FRET formats with far‑red tracers to minimize interference, it's HTS-ready across 96‑, 384‑, and 1536‑well plates with robust Z' ≥ 0.7 performance.