Thermo Scientific™ RapidFinder™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kits
Rapidly detect and differentiate Salmonella species, Salmonella ser. Typhimurium, and Salmonella ser. Enteritidis in a single test with Thermo Scientific™ RapidFinder™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kit. Confidently test accuracy and speed up product release with results in as little as 16 to 22 hours and validated workflows for poultry and pork meats, production environment samples and primary…
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Food technology
It's good value for money and highly efficient for the detection of microbes. It's great, efficient and useful for analysis.
Review Date: 17 May 2021 | Thermo Fisher Scientific
Increase your workflow efficiency while meeting regulatory requirements to detect the most common Salmonella serovars which cause disease in humans with the RapidFinder Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kit. The real-time PCR kit enables rapid, simultaneous detection and presumptive identification of Salmonella species, Salmonella Typhimurium, and Salmonella Enteritidis in a single well, with ISO 16140-2 and AOAC-RI validated workflows on a range of matrices including raw and ready-to-eat poultry and pork meat, food production environment samples, and samples from primary production.
- Faster decisions: earlier product release (negative results) and quicker interventions (positive results) due to simultaneous detection (three tests in one) and a reduced time-to-result compared with culture methods.
- Cost reductions: up to 50% consumable cost savings compared with running individual PCR tests for Salmonella species, Salmonella Typhimurium, and Salmonella
- Increased efficiency: save valuable technician time with single, non-proprietary enrichment, ready-to-use reagents, simple sample preparation, and simultaneous identification.
- Confident results: Applied Biosystems™ TaqMan™ Real-Time PCR probes provide a high level of specificity and sensitivity for industry-relevant serovars. Internal amplification controls give added confidence.
- Convenience: part of a range of end-to-end (enrichment to confirmation) testing solutions including all microbiology reagents and instruments, along with dedicated technical support worldwide.
RapidFinder™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kit includes:
- Pre-dispensed and ready-to-use reagents for cell lysis and release of DNA from bacteria present in enriched food and environmental samples.
- Pre-dispensed lyophilized PCR pellets containing target-specific primers, probes, Internal Positive Control (IPC), and reagents for multiplex real-time PCR using the prepared lysate.
For testing of Food and Environmental samples only.
Brochures
SureTect Real-Time PCR range for food pathogen detection
In this brochure, Thermo Fisher Scientific showcases the SureTect Real-Time PCR System and its full range of PCR solutions for food safety, authenticity, and quality testing.
SureTect E. coli O157:H7 PCR Assay method modification validation: Inclusivity and exclusivity
The Thermo Scientific™ SureTect™ E. coli O157:H7 species PCR Assay has been certified in accordance with the AOAC Research Institute Performance Tested Methods(PTM) Program to be used with the Applied Biosystems™ QuantStudio™ 5 Real-Time PCR Instrument to perform PCR and with Thermo Scientific™ RapidFinder™ Analysis Software version 1.0 or greater for data analysis. This application note details the inclusivity and exclusivity part of the validation.
SureTect E. coli O157:H7 PCR Assay method comparison study
In this application note, the Thermo Scientific™ SureTect™ E. coli O157:H7 PCR Assay method is compared against the United States department of agriculture food safety inspection service microbiology laboratory guidebook 5.09 reference method for raw beef and the ISO 16654:2001 reference method for fresh spinach.
Comparison of the Thermo Scientific RapidFinder Salmonella Multiplex PCR Kit with PCR kits from other suppliers
Identification of Salmonella serotypes is not only used for reporting during outbreaks but is also important in evaluating root causes of incidents on a local and global scale. Historically, the serotype identification of Salmonella has used serological techniques while modern approaches include molecular tests such as PCR for confirmation of serotype. The most commonly occurring Salmonella serovars in poultry and pork are Salmonella Typhimurium and Salmonella Enteritidis which account for the majority of US outbreaks.
Performance comparison of Shiga Toxigenic E. coli (STEC) multiplex molecular assays
In this application note, the sensitivity and specificity of the SureTect STEC Multiplex Assays is compared against a leading commercially available real-time PCR assay designed to detect O157:H7 and the top six STEC O serogroups in raw beef samples.
Use of an antibiotic-free enrichment medium for detection of Salmonella in cheese samples
In this application note, the performance of the SureTect Salmonella species assay is compared against the ISO reference method for detecting Salmonella from cheese samples using an enrichment medium without novobiocin.
AOAC-RI PTM and NF validation of the Thermo Scientific SureTect Salmonella species PCR Assay
This poster outlines AOAC-RI PTM and NF VALIDATION studies that demonstrate that the SureTect Salmonella species PCR Assay Workflow, including the QuantStudio 5 Food Safety System, is a suitable method for the detection of Salmonella species in a broad range of food and environmental surfaces, with superior or equivalent performance to the reference method.
Comparison of handling, time-to-result and validation parameters of commercially available PCR methods for Salmonella species detection and identification
This application note compares the Thermo Scientific™ RapidFinder™ Salmonella species, Typhimurium and Enteritidis Multiplex PCR Kit to four other commercially available PCR methods. PCR kits are compared for differences in necessary equipment, bench-space needed, number and duration for sample handling steps, method complexity and certification provided by AOAC-RI and AFNOR NF VALIDATION. The PCR methods are also compared for multiplex capability and total sample throughput.
Performance evaluation of Thermo Scientific RapidFinder Multiplex PCR Kits for the detection of Salmonella from primary production samples
This study evaluates the performance of the Thermo Scientific™ RapidFinder™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kit and RapidFinder Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Flex Kit in detection of Salmonella, including Salmonella Typhimurium and Salmonella Enteritidis, from PPS. Two methods were evaluated: a one-day method utilizing the Thermo Scientific™ KingFisher Flex™ Purification System (validated according to NF VALIDATION by AFNOR Certification) and a two-day method utilizing a secondary enrichment in MKTTn. Both methods were compared to ISO 6579:2017.
Screen for pathogens in four simple steps
In this video, discover how using the SureTect Real-Time PCR System you can quickly and accurately detect foodborne pathogens in a broad range of foods and associated samples, in just four simple steps.
It's easy to be sure with the SureTect PCR Pathogen Detection System
In this video, learn how the SureTect workflow can provide all the information you need for food safety, authenticity, and quality testing, using a system that is rapid, simple to implement, and easy to run on a day-to-day basis.
Impact of Salmonella on the global meat and poultry supply chain: Your questions answered
Watch this on-demand webinar to learn about the pathogenicity and transmission of Salmonella
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