SoftMax® Pro Microplate Data Acquisition & Analysis Software

This application note demonstrates the performance of NanoBRET™ technology for detecting protein interactions in living cells, using SpectraMax® Multi-Mode Microplate Readers. By leveraging the high sensitivity and flexible detection options of these instruments, researchers can accurately measure NanoBRET signals and analyze data with SoftMax® Pro Software. The note highlights assay validation across five reader models, showcasing their capability to deliver reliable results with enhanced signal quality and low background.

Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. However for biological applications involving small samples, such as next-generation sequencing and quantitation of DNA amplification products, sensitive methods are needed.

In this application note, Molecular Devices demonstrates how the Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA.

Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease conditions, with Luciferases being the most common of these.

In this application note, Molecular Devices demonstrates how the DuoLuc reporter assay and SpectraMax iD5 reader can be utilized to detect nuclear factor-κB (NF-κB) activation in mammalian cell models.

BRET (bioluminescence resonance energy transfer) is a technique for measuring protein-protein or protein-ligand interactions that involves the interaction of a bioluminescent donor and a fluorescent acceptor.

In this application note, Molecular Devices demonstrates how the SpectraMax® iD5 Multi-Mode Microplate Reader with SoftMax® Pro Software lets researchers acquire NanoBRET data using an optimized filter set and describe validation of the microplate reader with the NanoBRET™ PPI Control Pair.

In this eBook, Molecular Devices demonstrates how their SoftMax® Pro 7.1.1 GxP Software is their most secure software and can help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity.

Spheroids are small three-dimensional (3D) cellular microenvironments grown using a variety of specialized culture methods such as low-adhesion microplates. FUCCI (fluorescent ubiquitination-based cell-cycle indicator) spheroids have been developed to study cancer cell cycle progression as they allow the identification of cells in various phases of the cell cycle.

In this application note, Molecular Devices demonstrate how the MiniMax cytometer can easily acquire and analyze images of FUCCI spheroids.

Homogeneous time-resolved fluorescence (HTRF) detection can be challenging due to the optimization of instrument settings needed to meet assay requirements and detection limits. In this application note, Molecular Devices® demonstrates how its SpectraMax® microplate readers can be easily configured to be HTRF-ready with dedicated, validated modules to detect HTRF with improved sensitivity.

For researchers working in GLP or GMP laboratories, the SoftMax® Pro Software Validation Package provides the most comprehensive documentation and tools available to validate GxP protocols and data flow to ensure data integrity.

Difficult-to-kill bacteria have become a problem in hospital-acquired infections. Identifying compounds which kill these bacteria is of interest to many pharmaceutical companies. Developing and optimizing assays to screen for the efficacy of these compounds is a challenge faced by many microbiologists.

Stem cell-derived human cardiomyocytes have phenotypic characteristics and electrophysiological profiles similar to those of native human cardiac cells. Cardiomyocytes in culture are able to form a beating syncytium, which behaves similarly to native cardiomyocytes. Oscillation of intracellular calcium levels occurring with synchronized contractions of the cells can be monitored using a calciumsensitive dye, and treatment-induced changes in the pattern of oscillation can be monitored via changes in fluorescent signal over time.

Join Timothy Francis, from Fujifilm, as he explores the adaptation of microplate readers for bacterial endotoxin testing. This presentation delves into key techniques, including gel clot, turbidimetric, and chromogenic methods, with a special focus on the importance of kinetic measurement approaches. Learn how SoftMax Pro Software ensures compliance with GLP and GMP standards and discover a step-by-step protocol for the Kinetic Turbidimetric assay.