Cardiomyocyte Beating and Cytotoxicity Assays on the SpectraMax i3 Multi-Mode Microplate Platform with SpectraMax MiniMax Imaging Cytometer

This application note describes a cardiac beating assay using the SpectraMax i3 Multi-Mode Microplate Platform and the EarlyTox™ Cardiotoxicity Kit from Molecular Devices® for assessment of compound effects upon cardiomyocytes. Cardiomyocyte beating parameters were measured on the SpectraMax i3 Platform using a kinetic, well-by-well fluorescence read mode and analyzed using Peak Pro software algorithms in SoftMax® Pro Software. Sixteen analysis parameters, including peak count, peak frequency, peak amplitude, peak width, decay time, and several others, were calculated.