Measure p53-MDM2 protein interaction with NanoBRET technology
24 Feb 2021BRET (bioluminescence resonance energy transfer) is a technique for measuring protein-protein or protein-ligand interactions that involves the interaction of a bioluminescent donor and a fluorescent acceptor.
In this application note, Molecular Devices demonstrates how the SpectraMax® iD5 Multi-Mode Microplate Reader with SoftMax® Pro Software lets researchers acquire NanoBRET data using an optimized filter set and describe validation of the microplate reader with the NanoBRET™ PPI Control Pair.
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SoftMax® Pro Microplate Data Acquisition & Analysis Software
Molecular Devices®SoftMax ® Pro Software is designed to provide the simplicity, flexibility and power required for advanced data analysis. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Every step has been optimized for data acquired from a Molecular Devices microplate reader or data imported from another source to simplify analysis and reporting. FDA 21 CFR Part 11 compliance tools are available for regulated laboratories providing end-to-end chain of custody.