Shamrock 750
The Andor Shamrock SR-750 is the latest addition of the Andor family of spectrographs based on Czerny-Turner optical design. The Shamrock is available as a pre-aligned detector/spectrometer option allowing for seamless integration of software, electronics, optics and detector. There is also a fast and interactive graphical software interface allowing full control of all the spectrograph functions.
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The Andor Shamrock SR-750 is the latest addition of the Andor family of spectrographs based on Czerny-Turner optical design.
The Shamrock is available as a pre-aligned detector/spectrometer option allowing for seamless integration of software, electronics, optics and detector. There is also a fast and interactive graphical software interface allowing full control of all the spectrograph functions.
UV/VIS Spectroscopy of Membrane Proteins Encapsulated into Artificial Bilayer Lipid Membranes
An artificial bilayer lipid membrane system was employed, featuring the oriented encapsulation of membrane proteins in a functionally active form. Nickel-nitrilo-tri-acetic acid-functionalized silica nanoparticles of a diameter of around 25 nm were used to attach the proteins via a genetically engineered histidine-tag in a uniform orientation. Subsequently the proteins were reconstituted within a phospholipid bilayer, formed around the particles by in-situ dialysis to form so-called proteo-lipobeads (PLBs). With a final size of about 50 nm, the PLBs could be employed for UV/VIS spectroscopy studies, particularly of multi-redox center proteins, since effects of light scattering are negligible. Andor Technology imaging instruments were employed including the Shamrock SR-303i and Newton CCD and EMCCD Cameras.
EMCCDs for Spectroscopy
Learn about Electron Multiplying CCD technology principles for spectroscopy, with new applications including Photoluminescence, Raman, FRET, Single Molecule, Transient and Tunnelling spectroscopies.
