Vitamin B1 & B6 concentrated LC-MS Kit
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Intended Use
This LC-MS/MS kit is intended for the determination of Vitamin B1 & B6 in whole blood. The components in this kit must be used as stated in the user manual.
The kits are available in 2 versions: Starter Kit and Solvent Kit.
Summary
Mass spectrometry-based methods have been tested for the determination of Vitamin B1 and B6 using a Tandem Mass Spectrometry System (LC-MS/MS). Furthermore, chromatographic separation for both vitamins is critical and not easy. This method can be used for the routine analysis of Vitamin B1 and B6 in whole blood. Sample preparation is simple and rapid and analogous for the different biological matrices.
A six-point lyophilized whole blood calibrator as well as lyophilized blood controls are also available for quality assurance. Both components are added to the “Starter Kit” (30261535) or can be ordered separately. Two isotope-labelled internal standards, Pyridoxal-5’-phosphate (methyl D3) and Thiamin Diphosphate (methyl D3), are added to compensate for matrix effects and measurement variations. Samples are analyzed using positive ion electrospray in MRM mode for maximum sensitivity and selectivity.
Starter Kit
This Kit includes everything necessary for a first installation of the Vitamin B1 and B6 LC-MS/MS kit on an LC-MS/MS system. Includes all solvents for HPLC and sample extraction, Calibrators, Controls, as well as one HPLC column and consumables.
- Regulatory Status: RUO
- Kit size: 100
- Method: LC-MS
- LC-MS run time: 2.5 min
- Standard range: B1: 49-662 nmol/L, B6: 26-384 nmol/L
- Specimen / Volumes: 50 µL Whole blood.
Solvent Kit
The Solvent Kit includes a set of solvents to perform the sample extraction and the HPLC. Calibrators, Controls, Consumables, or an HPLC-Column are not included.
All components can be ordered separately.
- Regulatory Status: RUO
- Kit size: 100
- Method: LC-MS
- LC-MS run time: 2.5 min
- Standard range: B1: 49-662 nmol/L, B6: 26-384 nmol/L
- Specimen / Volumes: 50 µL Whole blood.
Manufactured by Diagnotix; Distributed by Tecan, IBL International GmbH.
