Series 3 HT Evaporator

Solvent removal is an essential process for sample preparation across a range of applications in many industries. Many sample formats and solvents are used with no single technique providing a universal solution, but despite the variety of specifics evaporation is well-understood and relatively uncomplicated.

Lyophilization, or freeze drying, has been used to dry samples in the laboratory for a number of years. Lyophilization is often a prefered method of drying as it achieves a very high level of dryness with low residual solvent levels and because of light, powdery finish of the sample enabling the sample to be easily removed and weighted out. Unfortunately there are a number of problems and limitations associated with Lyophilization. This application note presents the results of research done in the Genevac laboratory to develop the best solution to these problems.

Traditional concentration of reverse phase HPLC fractions to dryness in a Genevac HT evaporator is a fast and safe process. However, for certain compounds a fully dried result can be hard to achieve due to compound interactions with water boosting up the boiling point making drying difficult. Some compounds also tend to naturally form a gum or oil. In such cases, subsequent characterization by NMR normally indicates the presence of residual solvent, whilst samples which are not completely dry are of little use in biological assays since accurate weights cannot be obtained.This application note presents the Lyospeed™ method for avoiding these problems.

Drying high performance liquid chromatography (HPLC) purification fractions, principally comprising water and acetonitrile, is a routine yet essential task in many laboratories. The requirement is to dry the samples to a powder form, such that samples can be accurately weighed, easily sub-sampled and redissolved. Freeze drying is therefore the preferred technology, however, large scale traditional freeze driers may have difficulty processing the organic solvents which can boil out of the samples and damage the vacuum pump. This application note details the implementation of the Lyospeed™ method for fast lyophilization by Actelion Pharmaceuticals.

If you're carrying out HPLC purification, then at some point you'll need to dry a solution of Acetonitrile/Water or Methanol/Water. This is actually one of the most complicated solvent mixtures to dry, and many users, unaware of the best way to do it, take far longer than they need to, to dry their solutions. Genevac Evaporators have special features that enable you to get the job done quickly and effectively while all the time providing the unique Genevac "SampleGuard" protection that prevents your sample ever exceeding a safe temperature. This application note describes drying techniques that apply equally to Acetonitrile/Water or Methanol/Water.

Watch this video to see a demonstration of the Genevac Series 3 HT Evaporator. The Series 3 HT evaporator range represents the ultimate in solvent removal technology. Incorporating high performance vacuum pump, the latest touchscreen technology and a sleek ergonomic design, a Series 3 HT evaporator makes optimizing your evaporation processes effortless.

Genevac has announced the release of its HT Series 3 evaporators with Inert Gas Purge (IGP) option, enabling safe operation even when removing highly explosive solvents

Genevac announces a major new online video resource for any lab tasked with removing solvent from its samples