QIAseq Normalizer Kits
Reagents for normalization of NGS libraries containing intact P5/P7 sequences.
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The QIAseq Normalizer Kits offer a fast and easy workflow to adjust many different types of libraries to a fixed concentration (4 nmol/L). Once normalized, libraries can be pooled at equal volumes to achieve homogenous read representation without the need for library quantification prior to sequencing. The QIAseq Normalizer Kits enable streamlined NGS workflows that are cost effective and have a shorter processing time compared to conventional methods using classical library quantification and pooling techniques.
The QIAseq Normalizer workflows are compatible with virtually all libraries that can be sequenced on Illumina sequencing instruments. This includes QIAGEN as well as third-party library preparation workflows for Illumina sequencing instruments.
Features
- Produces normalized, ready-to-sequence libraries at 4 nmol/L
- Streamlined 30-minute benchtop protocol
- Seamless integration into many NGS workflows including QIAseq as well as virtually all third-party library preparation workflows for Illumina
- Produces dsDNA for stable, longer term library storage
- Fume hood not required
A library prep workflow using QIAseq FX DNA Library Kit and QIAseq Normalizer Kit on the Opentrons Flex
As the ambition of sequencing projects and capacities of sequencing platforms grows, labs are increasingly looking for ways to streamline laborious and time-consuming preparation of libraries. One way of achieving this is through automating the library prep workflow. Additionally, library prep workflows are becoming more efficient as a result of advancements in library prep chemistry, with features that support automation, shorter workflows and/or fewer pipetting steps. Explore the automation of QIAGEN’s QIAseq FX and Normalizer chemistries on the Opentrons Flex™ for efficient preparation of E. coli, human genomic and mixed microbial DNA libraries.
