Precellys® Evolution - Tissue Homogenizer

Tissue dissociation is vital for single-cell isolation, yet traditional methods often compromise cell quality. Explore this collection of application notes to learn how advanced mechanical dissociation technology improves reproducibility, cell viability, and workflow compatibility. This enables researchers to generate more reliable data, streamline preparation, and enhance performance across cellular analysis, disease modeling, and regenerative medicine.

In the past years, cannabis has attracted increasing attention due to the rising instances of its legalization for recreational use and for medical benefits. The sample preparation protocol is crucial for the success of most standard analytical methods used to study cannabis. The efficiency of 3D-beating technology is considered to be the gold standard for sample homogenization. This is the reason why it empowers the Precellys homogenizers range of Bertin Technologies.

In this white paper, Bertin Technologies presents optimized protocols to help researchers get high-quality molecules for their cannabis analysis workflows.

The extraction of nucleic acids (DNA and RNA) from murine samples is a standard practice for many biological applications. In this application note, Bertin Instruments presents a comparison of the DNA and RNA extractions obtained with samples that were homogenized with the Precellys 24 and the new Precellys 24 Touch. Bertin Instruments also presents how the efficient protocol of the Nucleic Acid Extraction kits of Bertin Bioreagents enables high yields of total DNA and RNA from mice tissue samples.

The overall success of a next-generation sequencing (NGS) workflow is greatly impacted by the various sample preparation steps. Obtaining adequate quantity and acceptable quality of nucleic acids from the sample is highly dependent on the efficiency of homogenization.

In this application compendium, we present best practices for sample homogenization based on the protocols developed and tested by scientists. Applied across diverse sample types, such as biological tissue, food, and fecal samples, these methods demonstrate how high-quality, high-yield nucleic acids can be obtained by choosing optimized bead-beating homogenization protocols with Bertin’s Precellys homogenizers.

The eBook covers:

• Robust and unbiased microbial RNA extraction workflow
• MicroRNA expression profiling of individual rat hypothalamic nuclei
• Transcriptomic analysis of cheese-ripening microbial communities
• Microbiota analysis of fermented milk samples with NGS
• Optimized protocol for viral metagenomics studies
• DNA extraction from frozen tumor samples

For thermo-sensitive molecules such as proteins, sample preparation is a critical step before proceeding to molecular downstream analysis. In this application note, Bertin Instruments highlights the key considerations in protein sample preparation.

In this study, protein precipitation and bioactive lipid extraction from rat skin tissues is reported. The combination Solid Phase Extraction and liquid chromatography-tandem mass spectrometry (LCMS/MS) approach was used to measure concentrations of lipid mediators in these tissues. Rat skin tissues were homogenized using CKMix50_7mL Precellys® Lysing Kit and Precellys® Evolution tissue homogenizer combined with Cryolys® cooling unit. In addition, temperatures of homogenates were measured to investigate if temperature of complete homogenate of rat skin tissues remained below 40°C.

Coral reefs are diverse underwater ecosystems held together by calcium carbonate structures secreted by corals. Coral reefs are built by colonies of tiny animals found in marine water that contain few nutrients. This research focuses on nutrient dynamics on coral reefs. The Burkepile lab in collaboration with the Moorea Coral Reef Long Term Ecological Research Lab collected a common macroalgae, Turbinaria ornata, at 190 points around the island of Moorea, French Polynesia. Nutrient hot spots were evaluated by analyzing the tissue content of dried Turbinaria for carbon and nitrogen as well as stable isotopes.

The biggest challenge when working with fibrous, elastic and tough samples is efficient sample processing. Furthermore, generation of heat causes degradation and denaturation. Here, Bertin Instruments describes the improvement of extraction yields of tough samples using the Precellys® evolution homogenizer, thanks to 3D-bead beating technology. When paired with the cryolys evolution cooling unit, this technology can also prevent degradation of heat-sensitive biological samples.

This best practices guide for protein sample preparation using the Precellys® Evolution presents two application notes and two specific protocols for protein extraction.

Leukotrienes (LTs) are inflammatory molecules which play an important role in triggering inflammatory diseases such as asthma, neurological diseases and diabetes. As a result, it is important to better understand their role and function in terms of disease progression and their potential value as biomarkers. This application note describes the use of the Precellys® Evolution in sample preparation.

In this video, hear how the Precellys® Evolution from Bertin Instruments is enabling CRO Genclis SAS to extract highly concentrated proteins, recognized by IgE antibodies in allergic patients, from difficult food samples.

In this video, discover the Cryolys® Evolution, a patented cooling system compatible with the Precellys Evolution tissue homogenizer. End-users may control both devices from a single interface, enabling them to benefit from greater performances in a minimal bench space. Cryolys Evolution also prevents thermo-sensitive molecules, such as RNA, siRNA, metabolites, enzymes or proteins, from heat degradation during homogenization process. These molecules are subject to more and more investigations, especially in the fields of cancer research and pharmacology. Cryolys Evolution’s patented technology allows to maintain a low temperature (0°C- 10°C) before, during and after the grinding process with Precellys Evolution.

A uniform homogenization of cells ensures comprehensive cell lysis and influences all downstream applications, especially in microbiomics. Emily Putnam, Assistant Manager of Services at Zymo Research, speaks about efficient homogenization and lysis conditions to study microbial communities. Using the Precellys system by Bertin Instruments reduced homogenization time down to 1 minute as compared to traditional methods that need 25 minutes.

The Precellys® Evolution is the universal tissue homogenizer gathering high flexibility and high efficiency for either routine or R&D projects. With spin speeds up to 10 000rpm, the Precellys® Evolution can accommodate 4 sizes of tube from 0.5ml to 15ml. The system also offers patented cooling unit for sensitive samples.

The Precellys® Evolution offers optimal robustness, safety, efficiency and flexibility. Especially designed for L3 laboratories, the Precellys® Evolution incorporates a unique figure-8 multi-directional motion, providing the same high level of energy and homogenization efficiency to all tubes.

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