Nanosight Pro

Viruses, such as adenoviruses, retroviruses, and adeno-associated viruses (AAVs), have been utilized as vectors in cell and gene therapy for decades. In biopharmaceutical research, gene therapy has become an area of continued growth, but is not yet a commercially viable drug platform.

Download this eBook to help strengthen your analytical workflows and accelerate viral vector characterization, development, and scale-up. Including a peer-reviewed article, application notes, and product guides, this eBook will steer you through the complexities of viral vector characterization to help you bring successful nanomedicines and nano-delivery systems to market, including:

• Characterization of recombinant rAAVs
• AAV attributes
• Enhanced characterization
• Multi-detection SEC in gene therapy
• Measuring the concentration of AAVs

In this white paper, Malvern Panalytical explores both the optimal conditions for extruding liposomes, and their stabilty under different conditions.

This whitepaper describes the characterization of gold-labeled Adeno-Associated Virus (AAV) and other small viruses by Nanoparticle Tracking Analysis (NTA). It details the method of gold-labelling the virus particles followed by using the NanoSight from Malvern Instruments to utilizes the properties of both light scattering and Brownian motion to obtain the particle size distribution of virus in liquid suspension. The paper concludes that this method can directly measure AAV particle concentration in a matter of hours.

This whitepaper describes rapid characterization of liposome-insulin receptor complexes by Nanoparticle Tracking Analysis using the NanoSight NS300 from Malvern Instruments. The NanoSight technology observes the Brownian motion of particles in suspension and calculates their size through the Stokes-Einstein equation. The paper concludes that the high resolution NTA using the NanoSight NS300 is a useful technique in analyzing the aggregation kinetics of liposome preparations.

This application note describes how Nanoparticle Tracking Analysis (NTA) can be used to determine filtration efficiency and study membrane fouling. The results can be used to optimize microfiltration in water treatment applications.

This white paper discusses the role of Nanoparticle Tracking Analysis (NTA) in nanomedicine and reviews the specific mechanisms by which such nanoparticles are designed and formulated. Nanoparticles, and many of their subsets, have been revealed to have important biomedical applications; one in particular being drug delivery. Particles can be engineered to specifically target diseased cells, such as cancer cells, allowing for targeted drug therapy with reduced collateral effects.

At the same time as an increasing interest in, and rapid development of, a wide range of materials and products containing nanoscale structures and engineered nanoparticles, awareness has grown that the longer term potential toxic effects of such materials and their potential environmental impact are poorly understood. Existing methods have been assessed and new methods sought by which such materials could be analyzed on a routine basis during development and manufacture. Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension, and shows potential for the analysis of a range of environmental nanoparticle contaminants.

This white paper provides an overview of the commonly found nanoparticles present in environmental samples, as well as suitable techniques for each species. Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension, and shows potential for the analysis of a range of environmental nanoparticle contaminants.

This white paper reviews the use of Nanoparticle Tracking Analysis for the characterization of viruses and viral vaccines. Nanoparticle Tracking Analysis allows suspensions of nanoparticles to be visualized, sized and enumerated based on a particle-by-particle basis, its ability to determine the concentration and direct number frequency based particle size distribution profile means that virus preparations in particular can be studied in higher detail.

This white paper highlights the growth in the use of the technique of Nanoparticle Tracking Analysis as applied to nanoparticles and nanomaterials production and analysis, from the very first reports on nanoscale silver and gold.

In this video, Oliver Huseyin from MIP Discovery demonstrates his use of NanoSight Pro for developing and quality-assuring molecularly imprinted polymers (MIP) used in sensors and COVID-19 lateral flow tests. He relies on NanoSight Pro to accurately size his MIP population, ensuring effective MIP conjugation and batch consistency. Huseyin highlights the quick setup and analysis process with NanoSight Pro, emphasizing its advantages over NS300 technology.

In this video, Malvern Panalytical introduces NanoSight Pro, a cutting-edge solution for characterizing bio and nanomaterials. NanoSight Pro provides quick and detailed access to high-resolution size and concentration data, offering particle-by-particle tracking precision through Brownian motion. Its unique optical system ensures repeatable and superior data quality, enabling a deep understanding of complex systems. Combined with fluorescence capabilities and powered by machine learning, NanoSight Pro automates workflows, eliminating subjectivity and human error.

In this video, Malvern Panalytical provides highlights from the launch event of the NanoSight Pro. NanoSight Pro provides access to high-resolution size and concentration data, revealing individual nano-scale traces through Brownian motion. This technology offers particle-by-particle tracking precision, decoding the complexity of various systems. Enhanced by its unique optical system, NanoSight Pro ensures repeatable detection and superior data quality.

This year’s Reviewers’ Choice Award for Analytical Science Customer Service of the Year went to Malvern Panalytical. In this video, Dr. Anne Virden, Product Manager Micro Materials at Malvern Panalytical, highlights the value of user reviews. Virden explains how customers are always at the center of what the company does and how product reviews on SelectScience enable Malvern Panalytical to truly understand their customers’ needs.

Take a look at the reviews left by scientists around the world for Malvern Panalytical’s products and leave your own reviews on the lab equipment you use.

Watch this presentation by Dr. Anne Virden and Dr. Debbie Huck-Jones, Malvern Panalytical, titled: Physico-alchemy: Optimizing drug formulations through measurements at the microscale. This talk was presented at the SelectScience® Virtual Analytical Summit 2021.

In this video visualize and measure nanoparticle size and concentration with Nanoparticle Tracking Analysis (NTA) with NanoSight from Malvern Instruments. High resolution particle size distribution data are obtained used the Brownian motion of the nanoparticles in liquid suspension illuminated by a laser light source.

The Malvern NanoSight NS300 Instrument provides an easy-to-use, reproducible platform for nanoparticle characterization using Nanoparticle Tracking Analysis. The NS300 allows rapid, automated analysis of the size distribution and concentration of all types of nanoparticles from 10nm to 2000nm in diameter, depending on the instrument configuration and sample type.

Watch this video to learn how the Malvern NanoSight range of instruments utilize Nanoparticle Tracking Analysis (NTA) to characterize nanoparticles from 10nm -2000nm* in solution.

Dr. Vikramsingh Gujar, from Northwestern University, discusses his research on inflammatory disorders and why effective knowledge share is vital for scientific advancement

Take a look at what other researchers and scientists are saying about cutting-edge technologies in drug discovery

Malvern demonstrates ongoing commitment to extending and supporting the application of NanoSight