MA900 Multi-Application Cell Sorter

In this application note, the MA900 cell sorter was used for analysis and sorting of a 12-color immunophenotyping panel. Human peripheral blood mononuclear cells (PBMCs) were stained with reagents for analysis of common T-cell, B-cell, NKcell, and monocyte subsets. These subsets were identified using different gating strategies, and sort performance was evaluated by performing 4-way sorting of T cells, NK cells, B cells, and monocytes, followed by post-sort analysis.

Multi-user laboratories sort a variety of cells, which demands flexibility in a sorter’s optical design. Previously, the only approach to meet this demand was to purchase a cell sorter preconfigured with multiple parameters across many lasers. Though many fluorescence channels in such a flow cytometer might not be used simultaneously, labs could change their panel design without having to reconfigure or upgrade their instrument for each application. The MA900 is the first cell sorter to offer an affordable option to labs that need flexible detection capability.

Thursday, December 4, at 16:00 GMT | 17:00 CET | 11:00 EST | 8:00 PST

Advances in cell therapy, cancer biology, and immunology increasingly require analytical tools that can accommodate complex models and functional assays. Emerging applications involving large cells, aggregates, and “lab-on-a-particle” platforms demand reliable characterization and sorting of these samples. Conventional flow cytometers have challenges with larger particles such as clogging, low sort efficiency and reduced recovery.

In this webinar, we will introduce the new Large Particle Sorting Upgrade for the SH800 and MA900 Cell Sorters from Sony Biotechnology, designed to expand compatibility with large and/or structurally complex sample types. Attendees will gain an overview of the key software features, along with data demonstrating their impact on sort recovery and purity. We will also share case studies highlighting applications in cell-cell interaction studies, functional characterization of immune cells, and spheroid sorting.

Join us to learn how this new capability enables researchers to seamlessly integrate next-generation large-particle assays into routine flow cytometry workflows.

Learning objectives:

• Understand the new features available on Sony benchtop cell sorters that support sorting of large particles to achieve high purity and recovery
• Learn how key applications in immune cell analysis, cell-cell interactions, and spheroid sorting can be improved
• Discover new large particle technologies which are expanding the ways researchers characterize and sort cells

Who should attend?

This webinar is ideal for researchers, scientists, and core facility managers working in cell therapy, cancer biology, and immunology who are looking to expand their flow cytometry capabilities. It will also benefit those involved in large-particle or 3D culture applications such as spheroids, organoids, and hydrogel systems.

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes. If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

Wednesday, November 5, at 16:00 GMT | 17:00 CET | 11:00 EST | 8:00 PST

Advances in cell therapy and immunology increasingly demand technologies that move beyond surface marker profiling to capture the true functional properties of single cells. This webinar showcases the use of the Nanovial technology from Partillion Bioscience in conjunction with the new Large Particle Sorting Option available for SH800 and MA900 cell sorters from Sony Biotechnology.

Nanovial technology uses a microparticle platform that captures cytokine secretion while preserving cell viability, thus enabling direct linkage of phenotype to function for deeper insights into immune biology and therapeutic development.

This webinar will discuss the use of Nanovials for:

• high-throughput screening of CAR libraries and the recovery of rare antigen-specific TCRs with demonstrated tumor-targeting efficacy; and
• immune profiling of T-cell subsets and simultaneous measurement of surface markers and secretion of resting T-cell phenotypes.

Our expert speakers will also introduce the new Large Particle Sorting Option available for Sony cell sorters, which are suitable for these types of applications. This software feature is designed to expand compatibility with Nanovials, organoids, and hydrogel platforms, making it easier than ever to integrate functional assays into routine flow cytometry workflows.

Key learning objectives:

• Understand how Nanovial technology links T-cell surface phenotype with cytokine secretion.
• Explore applications in engineered receptor discovery and immune profiling.
• Learn how the Large Particle Sorting Option for the SH800 and MA900 supports Nanovials, organoids, and other large particle-based assays.

Who should attend?

Researchers working in the areas of immunology, oncology, and cell therapy who are developing CAR/TCR platforms or studying functional immune responses. The webinar is also useful for core facility managers and lab leaders seeking new sorter capabilities.

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

Immunotherapy has revolutionized treatment options for multiple solid tumors, including non-small cell lung cancer (NSCLC). However, these approaches are effective in only 20–30% of NSCLC patients. Current immunotherapies do not consider or target B cells or tertiary lymphoid structures (TLS) despite their predominance in the tumor microenvironment and key role in the adaptive immune response. B cells can help generate potent, long-term immune responses against tumor cells by educating T cells within TLS and producing tumor-specific antibodies.

Join this webinar to hear how Dr. Tullia Bruno’s team at the University of Pittsburgh Medical Center is evaluating the role of B cells and TLS in lung cancer. Using a multi-omics approach that combines cytometry, single-cell RNA sequencing, and multispectral imaging, the team has examined the determinants of TLS heterogeneity in healthy and patient lung tissue samples.

Who should attend?

This webinar will provide insights to researchers who are studying the role of B cells in tumor-response mechanisms. Participants will learn more about how to integrate multi-omic approaches to evaluate lung cancer biomarkers and targets for early intervention in COPD patients and immunotherapeutic strategies for NSCLC.

Key learning objectives

• Understand the role of B cells and tertiary lymphoid structures in the tumor microenvironment
• Learn how flow cytometry, single-cell RNA sequencing, and multispectral imaging can be used together to identify key biomarkers in lung cancer immune response

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary for continuing education purposes.

Susceptibility to many diseases is regulated by genetic variation, which occurs most often in non-coding regulatory regions of the genome, including enhancers and silencers. Functionally, enhancers and silencers fine-tune gene expression with cell type and/or context specificity. Therefore, understanding disease mechanisms may be improved by assessing dynamic changes in enhancer and silencer function using highly purified cell populations from disease-relevant tissues. Data from these approaches can nominate candidate transcription factors and upstream signaling pathways responsible for disease-induced gene expression changes.

Join this webinar to learn how sorted hepatic cells and nuclei were used with RNA-seq, ATAC-seq, and ChIP-seq to decode gene expression of Kupffer cells, the major liver resident macrophage. Kupffer cell differentiation, niche-fitness, and transcriptional reprogramming during nonalcoholic fatty liver disease will be discussed.

Key learning objectives

• Learn about cell type-specific gene regulation and the relevance to understanding disease
• Understand strategies that include cell sorting and single cell transcriptomics for developing in vivo gene regulatory atlases for disease-relevant cell types
• Hear how collaborative interactions of LXR and ATF3 control disease-associated gene expression in Kupffer cells

Who should attend

This webinar will provide insights for researchers who want to learn strategies for decoding disease through combinatorial application of cell sorting and genomics technologies. This strategy will be presented through the lens of hepatic macrophages in health and disease.

The availability of fresh human tissue remains an impediment to the study of solid tumor indications. The generation of a highly-annotated™ cryopreserved dissociated tissue biobank provides the opportunity to develop highly focused study cohorts to understand the complex cellular heterogeneity of human tissues. Performing “-omics” analyses on heterogeneous biological tissue samples can, however, lead to biased results, particularly when the target cells are present in relatively low numbers.

Join this webinar to understand how Discovery Life Science’s cell services laboratory processes primary human tissue for downstream analytical evaluation. Sony’s MA900 cell sorter is an integral part of multiple workflows that support both the analyses of cell populations and preparation of specimens for multiple downstream applications. Experimental data showing single cell resolution of protein expression and results using 10x Genomics single cell RNA-seq (scRNA-seq) technology will be discussed.

Learning objectives:

• Learn how the dissociation and cryopreservation of primary solid tissues can accelerate preclinical pipelines
• Review the workflow used by Discovery that combines cell sorting, cellular debris clean up, and immunophenotyping of primary human tissue into one step to generate single cell data
• Discuss the latest approaches such as 10x Genomics and custom cell culture studies used in downstream single cell applications

Who should attend:

This webinar will provide insights to researchers who want to learn how to utilize cryopreserved primary solid tissues to accelerate preclinical pipelines. Attendees will learn how to optimize the samples from biobanked tissue using cell sorting to perform single cell transcriptomics studies.

Chimeric antigen receptor (CAR)-T cell therapy is a new type of “living drug” that has proven to be a powerful immunotherapy for hematologic malignancies. However, this success has not yet been transferred to solid tumors due to inefficient homing of CAR-T cells, the immunosuppressive tumor microenvironment (TME), and on-target off-tumor toxicities due to the shared epitopes on healthy tissues. The tumor associated antigen carbonic anhydrase IX (CA-IX) is overexpressed on clear cell renal cell carcinomas (ccRCC) and many other solid tumors.

Join this webinar to learn how engineered anti–CA-IX targeted CAR-T cells secreting human anti-programmed death ligand 1 (PDL-1) antibodies at the tumor site restore active anti-tumor immunity. An elevated expression of pro-inflammatory genes, downregulation of inhibitory immune modulator genes, and less regulatory T-cell differentiation of 4-1BB are observed during the CAR-T mediated response. In addition, anti–PDL-1 secreting CAR-T cells exhibit superior efficacy and less exhaustion and reverse the TME.

Key learning objectives

• Learn how engineered CAR-T cells are purified using the microfluidics chip based MA900 cell sorter for downstream functional assays.
• Review the workflow for evaluating the efficacy and long-term persistence of CAR-T cells in preclinical studies and for profiling enriched tumor infiltrating lymphocytes using single cell RNA sequencing.
• Understand how ex vivo engineered T cells represent an innovative approach to achieving a durable, long term, complete response against solid tumors.

Who should attend?

• This webinar will provide insights to researchers who want to learn how CAR-T cells secreting PDL-1 antibodies can be engineered for targeting solid tumors such as ccRCC.

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Automated cell sorters provide streamlined workflows for instrument and sort setup, enabling researchers of all expertise levels to intuitively operate them. Multi-user labs and shared facilities often sort different cell types including biohazardous samples and therefore need cell sorters that address their operational challenges.

The Sony MA900 Multi-Application Cell Sorter brings ease-of-use to a variety of applications performed in multi-user labs. Anchored on the utility of an exchangeable microfluidics chip, it eliminates the need for precision handling of nozzles, O-rings, and manual troubleshooting of sample clogs. Seamless system calibration with chips of different sizes reduces downtime between users sorting different cell types.

Key learning objectives

Join this webinar to learn how the MA900 cell sorter offers a streamlined approach to a variety of sorting applications. In this webinar you will:

• Learn how an automated and streamlined sort setup can increase the capabilities within a multi-user lab
• Review how the MA900 enables seamless setup and exchange of different chips without instrument downtime between users
• Understand how the MA900 system can be maintained in multi-user labs for successful, high-throughput, and gentle sorting of various cells

Who should attend?

This webinar will provide insights to researchers who are new to sorting or who operate shared flow facilities on how the Sony MA900 can support varied user needs with automated functions and guided workflows for sorting.

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Particulate contamination is an important consideration in ophthalmic applications, pharmaceutical production, and even more so in cell and gene therapy production and delivery. Every source of contamination must be considered in the biomanufacturing process, drug formulation, and drug delivery. One potential source of contamination is single-use systems (SUS) and components.

In this webinar, learn about the risks of potential contamination and how to monitor and mitigate these risks. Several key aspects of particle contamination control will be reviewed, including:

• Technical and regulatory concerns
• Clean manufacturing environment
• Continuous testing

Key learning objectives

• Understand regulatory expectation on visible and sub-visible particulates
• Learn how to test for particulate contamination
• Discover manufacturing practices to minimize particle contamination
• Learn how to stay ahead of emerging regulations and standards

Who should attend?

• Scientists and engineers working in biomanufacturing, cell therapy, gene therapy, SVP/LVP, and ophthalmic production
• Validation and qualification experts
• QA experts for single-use consumables
• Single use systems and consumables manufacturers

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

In this SelectScience interview, Professor Gianluca Matteoli from KU Leuven's Lab of Mucosal Immunology (Translational Research Center for Gastrointestinal Disorders - TARGID) discusses the interplay between immune cells, gut homeostasis, and potential new therapies for conditions like inflammatory bowel disease (IBD) and food allergies. Explore groundbreaking research on gut immune system regulation and chronic inflammatory diseases, and learn how advanced flow cytometry and patient-derived cells are driving this translational research.

The Flow Cytometry Core Facility at Columbia Stem Cell Initiative (CSCI) serves over 60 labs and supports a wide range of research fields, including neuroscience, cancer biology, immunology, bioengineering, and orthopedics. With a user base of approximately 400 independent researchers, the facility offers advanced flow cytometry and single-cell analysis services. In this video, Michael Kissner highlights the importance of providing users with independent access to technologies and emphasizes the significance of system design, usability, and automation in selecting equipment. Here, Kissner highlights how the facility has found great success in this endeavour with the Sony MA900 instruments, praising their performance, ease of use, and robustness.

See how the Laboratory of Mucosal Immunology at KU Leuven uses flow cytometry and cell sorting to advance our understanding and uncover new therapeutic targets for chronic gastrointestinal diseases

Learn about the pioneering work of the Columbia Stem Cell Initiative and how technological advancements are helping drive scientific breakthroughs at its Flow Cytometry Core Facility

Watch this on-demand webinar to discover the role played by TGF-ß in the chronic immune reaction in severe cases of COVID-19

Two flow cytometry experts share the new capabilities and diverse applications of the technology for deeper biological insights

Watch this on-demand webinar to learn how the dissociation and cryopreservation of primary solid tissues can accelerate preclinical pipelines

Gain insights on topics from laboratory ergonomics, quality control, cell counting, mRNA vaccines, and more

Gain insights on a range of current topics, including clinical microbiology, vaccine design, cell viability and more

An insider’s view on leveraging the latest single-cell technologies, including cell sorting and single-cell sequencing, to understand what drives response or resistance in checkpoint therapies

Learn how to optimize sample preparation and obtain quality data from a multiplexed, single-cell sequencing assay in this on-demand webinar

Join us on Thursday, October 17, for a masterclass on how to achieve optimal results from cell sorting