Sorting a 12-color immunophenotyping pannel

6 Oct 2019

In this application note, the MA900 cell sorter was used for analysis and sorting of a 12-color immunophenotyping panel. Human peripheral blood mononuclear cells (PBMCs) were stained with reagents for analysis of common T-cell, B-cell, NKcell, and monocyte subsets. These subsets were identified using different gating strategies, and sort performance was evaluated by performing 4-way sorting of T cells, NK cells, B cells, and monocytes, followed by post-sort analysis.

MA900 Multi-Application Cell Sorter

Sony Biotechnology

The MA900 from Sony meets the needs of most sorting applications, supporting 12 fluorescence parameters and 4-way sorting. Powerful, modern technologies built into the MA900 system include a patented micro fluidic chip-based design, comprehensive fluidic controls, and advanced automation that dramatically simplifies operation to make sorting less subjective and improve reliability.

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Sorting a 12-color immunophenotyping pannel