Gemini pH Flexible LC Columns
Setting the Standard for pH Method DevelopmentRugged reversed phase HPLC columns that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations. Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity Expect longer column lifetime with patented TWIN-NX™ technology Ideal for analytical and preparative separations of basic and acid…
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All perfect
Analyze
All was perfect. The product was pretty good and application to
Review Date: 27 Sept 2023 | Phenomenex Inc
very excellent column for research and development
Drug analysis by HPLC
Column is very useful with a wide pH range of mobile phase. Column life is also excellent after so many injections. Separation of peaks are very nice.
Review Date: 2 Apr 2022 | Phenomenex Inc
Would advise to have one in-house at any time.
Small molecules pkpd
Easy to use column with reliable performance over a long period of time and use.
Review Date: 26 Feb 2019 | Phenomenex Inc
A great column for small molecules with ionizable groups.
Analysis of small molecules
These columns can be used over a wider range of pH, which makes them much more versatile than the classical silica-based columns. Furthermore, they come in a brad range of bonded phases, particle sizes, pore sizes and dimensions. i found the Gemini SAX particularly useful because I can combine both the ionic strength and the pH of the mobile phase, to impact the selectivity, thus achieving optimal resolution of all my analytes with shorter run times.
Review Date: 27 Jul 2018 | Phenomenex Inc
I have worked with Phenomenex Gemini C18 and Phenomenex Phenyl C6 and obtained very good separation of compounds with aromatic rings, namely phenolics in plant materials (mobile phase of 01.% formic acid + acetonitrile). Phenyl C6 is better if you aim a good selectivity between isomers.
Review Date: 1 Sept 2014 | Phenomenex Inc
steroids
I have some compounds that I can measure only at high pH. This column is a good choice for longer column lifetime.
Review Date: 15 Aug 2013 | Phenomenex Inc
Perfect company, good business and cooperation. Easy to use columns, high resolution, easy to clean. Will recommend to my colleagues and partners with pleasure and without hesitation. I believe it has an excellent future.
Review Date: 7 Aug 2013 | Phenomenex Inc
I like to use this column because it has good selectivity. The life time is long. It is available for pharmaceutical molecules. The contact person is helpful.
Review Date: 7 Aug 2013 | Phenomenex Inc
pharmaceuticals
I have always been happy with Phenomenex columns. The columns work as advertised and if there is any problem, Phenomenex is always willing to provide post sales support.
Review Date: 28 May 2013 | Phenomenex Inc
drug analysis
These columns are very easy to use. They are versatile and can analyze a wide variety of compounds. I use them for environmental samples and for multiple drugs whether they are acidic or basic.
Review Date: 28 May 2013 | Phenomenex Inc
Setting the Standard for pH Method Development
Rugged reversed phase HPLC columns that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations.
- Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity
- Expect longer column lifetime with patented TWIN-NX™ technology
- Ideal for analytical and preparative separations of basic and acidic compounds
Who needs Phenomenex's Gemini HPLC columns? Method Developers: Extreme pH method flexibility Reproducible performance Purification Chemists: Purify bases at high pH Increased loading Increased retention Increased resolution Lab Managers: Extended column lifetime Consistent results.
Brochures
Capillary and trap column solutions for microflow LC
In this product brochure, Phenomenex showcases its range of high-quality reversed phase selectivities in capillary and trap formats. These materials are designed to allow you to achieve greater separation power for all your micro LC applications, are compatible with multiple system types, and offer a wide range of selectivities.
Phenomenex Pharmaceutical Analysis Brochure
This brochure provides information on how Phenomenex products can help you achieve resolution, ruggedness and reproducibility in your formulations assays.
Rapid LC-MS/MS method for monitoring bio-relevant levels of per- and polyfluoroalkyl substances (PFAS) in serum
In this application note, a quantitative workflow for the analysis of 22 per- and polyfluoroalkyl substances (PFAS) in serum was developed using a Gemini™ 3µm C18 column coupled with a SCIEX QTRAP® 6500+system. This targeted screening workflow provides a fast analytical method capable of accurately quantifying sub-nanogram per mL levels of PFAS in the human body.
Per- and polyfluoroalkyl substances are pervasive compounds used in a variety of industrial applications and found in a wide range of consumer products such as cookware, stain repellent, flame-retardant, and coatings. PFAS are considered environmental factors due to their persistent and bio accumulating nature. Bioaccumulation of PFAS in the human body resulting from environmental exposure is a growing public health concern. Recent studies have linked PFAS exposure to adverse health outcomes including:
- childhood health complications
- reduced kidney function
- thyroid disease
- hormone suppression
- decreased fertility
- increased cholesterol levels
- diabetes
Reducing PFAS interferences during human plasma and serum analysis with accurate mass spectrometry
In this application note, Phenomenex presents experimental data on the reduction of per- and polyfluorinated (PFAS) interferences in human plasma and serum analysis. PFAS are well known anthropogenic contaminants and are detected worldwide in most environmental media, including air, water and soil. Widespread human exposure has resulted in nearly ubiquitous detection of PFAS in human serum. In order to identify the potential health risks PFAS present to humans, accurate and robust analytical methods are required.
In this analysis, a Gemini™ C18 column coupled with a high resolution, high mass accuracy mass spectrometer was used to mass resolve perfluorooctane sulfonate (PFOS) and perfluorohexane sulfonate (PFHxS) from serum interferences. The EPA 533 PFAS list was selected as the target analytes. The interferences were characterized using their fragmentation patterns and through comparison with MS/MS libraries. Finally, the serum interferences were chromatographically resolved using a 6.5 min gradient.
PFAS extraction by LC-MS/MS using Strata PFAS for a stacked SPE solution
Download this application note to find a method where an LCS sample was extracted with Strata PFAS and analyzed on an LCMS/MS system using a Gemini® 3 µm C18 HPLC column under the conditions described in a previously published GenX Application Note.
The ultimate guide to HPLC/UHPLC: Reversed phase selectivity
In this step-by-step selectivity guide, Phenomenex provides an easy overview of the reversed phase HPLC/UHPLC options available to you. Download to understand the differences between the columns available, and select the right solution for your specific method and goals.
Perfluoroalkyl Substances (PFAS) Testing Guide
Explore HPLC column choices and sample preparation options for diverse sample matrices, separation selectivities, and workflows. Download this application note to find the latest word on PFAS analysis - but certainly not the final - on this rapidly expanding field of investigation.
The Comprehensive HPLC/UHPLC Reversed Phase Selectivity Guide
The step-by-step guide gives an overview of the reversed phase HPLC/UHPLC options available. It explains the differences between the range of columns and describes how to choose the right solid support and selectivity for your specific method and goals.
Comparison of Reversed Phase HPLC/UHPLC Column Selectivity
This application note graphically compares a range of Phenomenex® columns by hydrophobicity, steric interaction, hydrogen bonding capacity and cation selectivity. It lists columns that are best suited for separating different types of compounds, such as hydrocarbons and polar basic compounds. It also gives advice on choosing a solid support system.
A Fast and Effective Approach to Analyzing Hormones in Drinking Water Using SPE and LC/MS/MS
This application note illustrates a method for steroid hormone analysis from drinking water using an optimized LC/MS/MS method. Analysis time is reduced from 50 minutes down to about 15 minutes while maintaining excellent linearity (0.99 R2 value or greater for each compound) and low minimum reporting level MRLs. The method presented uses a Gemini® 3 μm NX-C18 HPLC column, which delivers stability at pH 1-12. This improved method should greatly increase lab efficiency and productivity.
A Sensitive, Specific, Accurate, and Fast LC/MS/MS Method for Measurement of Nicotine, Cotinine, 3-Hydroxycotinine, Nornicotine and Anabasine in Human Urine
This application note illustrates a method simultaneous analysis of nicotine, two of its major metabolites, cotinine and 3-hydroxycotinine, as well as nornicotine and anabasine from human urine samples. The method described uses Strata™-X-C solid phase extraction (SPE) cartridges for sample clean-up and concentration, followed by fast (<6 min) LC/MS/MS analysis using a Gemini® NX-C18 column.
HPLC-UV Analysis of Vitamin C from Tablets and Soft-Gels
The verification of label claim data in nutraceutical formulations has come under scrutiny by the FDA. Manufacturers and contract testing labs alike are looking for accurate and scientifically valid methods that are suitable for use with different formulations. There were two primary goals of this project: firstly, to determine an accurate and reproducible means of analyzing Vitamin C and secondly, to demonstrate the suitability for analysis of two commercially available formulations of Vitamin C.
Sample preparation and chromatography considerations in routine and advanced PFAS LC-MS/MS workflows
Understanding particle morphology and column chemistry contributions for HPLC and UHPLC analyses of PFAS
