Eppendorf BioBLU® Single-use Vessels

Managing the weight of bioprocessing data can often overwhelm laboratories, but effective data management is both achievable and critical to successful biopharmaceutical development.

This SelectScience application eBook highlights advanced instruments and digital tools that integrate seamlessly into laboratory infrastructure, enabling continuous monitoring and streamlined data management. With remote accessibility, these solutions empower scientists to maintain oversight and control of bioprocessing data beyond the physical lab environment.

Download this SelectScience guide to learn:

• How to conduct accurate and efficient real-time monitoring of Chinese hamster ovarian (CHO) cultivation using the BioNsight®
• Case studies using automated technologies for sampling CHO and E.coli cultures to understand growth conditions, and improve experimental throughput.
• The potential of Raman spectroscopy to accurately track critical process parameters

Resource details:

• Document type: SelectScience guide
• Page count: 49
• Read time: 72 mins
• Edition: 1st

The potential leakage of toxic or inhibitory chemicals from plastic materials from single-use bioreactors is increasingly known within cell culture. Leachables are particularly problematic, as they can migrate from the plastic into the process fluid under process conditions and can inhibit cell growth or affect the final product quality. By strictly adopting the 'DECHEMA Recommendation for Leachables Studies – Standardized cell culture test for the early identification of critical films', Eppendorf demonstrates the absence of negative impacts caused by leachable compounds from the X-ray irradiated material on growth, viability, and metabolic profile of the CHO cell line tested.

In this guide, Eppendorf provides detailed information on optimizing cell and gene therapy workflows and options for scaling up cell cultures with advanced bioreactor systems. Cell and gene therapies are emerging fields of transformative medicine where diseases are treated by restoring or reconditioning cells or genes. Cells and genetically engineered cells demonstrate vastly different properties than traditional medicines and surgery. Akin to “living drugs”, they can heal and replace diseased organs and thus have the potential to provide curative therapies for various diseases.

In this application note, Eppendorf demonstrates the capability of fed-batch cell culture and the flexibility of the SciVario twin platform, including its capability to control two bioreactors in parallel or individually across a wide range of vessel sizes.

There is growing interest across the food and feed and biofuel industries in microaerobic fermentation, a process occurring at close to anaerobic conditions, but still requiring small amounts of oxygen. This application note describes the microaerobic fermentation of a probiotic strain, Lactobacillus acidophilus, at a very low oxygen level using BioFlo® 120 and 320 control stations.

In this application note, Eppendorf explains the differences between batch, fed-batch, and continuous fermentation and how these influence culture growth, using E. coli fermentation processes as an example.

To investigate the methanol metabolism of Methylomicrobium alcaliphilum researchers at the San Diego State University analyzed bacterial growth and substrate utilization in chemostat mode. They used a parallel DASbox® Mini Bioreactor System equipped with BioBLU 0.3f Single-Use Vessels. Parallel experimentation using single-use equipment saved time and reduced possible sources of fluctuation between replicates.

Vero cells are anchorage-dependent cells that are widely used as a platform for viral vaccine production. In this application note, Vero cells are grown on the alternative attachment matrix, Fibra-Cel®. A high cell density is achieved, demonstrating great potential for Vero-cell-based vaccine production using Fibra-Cel packed-bed vessels.

The development of industrial bioprocesses is time-consuming and costly, yet the employment of single-use equipment alongside parallel experimentation considerably reduces development time. Parallel processing enables the testing of multiple parameters in one run, and single-use bioreactors omit the need to carry out extensive sterilization procedures. In this application note, Eppendorf, in collaboration with Dieter Eibel and his team at Zurich university, evaluate the DASbox Mini Bioreactor parallel cell culture system with BioBLU single-use vessels for their suitability in bioprocess development.

This application note demonstrates the successful expansion of hMSCs-BM in an Eppendorf DASbox Mini Bioreactor System equipped with BioBLU 0.3c Single-Use Vessels. Furthermore, expansion in BioBLU 0.3c Single-Use Vessels did not affect the hMSC multipotency and they conserved their ability to differentiate into osteocytes and chondrocytes.

Recognized by scientists worldwide for the second year running, Eppendorf again won the Reviewers' Choice Award for best customer service. Watch the video to discover Dr. Andreas Hochberger's thoughts on winning the award.

Discover how the latest innovations in upstream bioprocessing are serving the cell and gene therapy industry

Visitors to LABVOLUTION 2019 can keep up to date with important information and exhibition highlights with the “Meet Eppendorf” app

From cell signaling to single-cell isolation to cell culture practices, we bring you the latest in cell biology to accelerate your research

Life science company Eppendorf introduces the BioFlo 120, their latest bench scale fermenter/bioreactor system for research and development

Eppendorf expands its portfolio of rigid-wall single-use vessels for fermentation