Eppendorf Expands Single-Use Vessel Portfolio for Microbial Fermentation

Eppendorf expands its portfolio of rigid-wall single-use vessels for fermentation

30 May 2016
Alex Waite
Editorial Assistant

The newly launched Eppendorf BioBLU® 3f was developed specifically for microbial bioprocessing in working volumes of 1.25 to 3.75L, extending the full range of operation for single-use fermentation from 60 mL to 3.75L.

Eppendorf BioBLU f Single-Use Vessels specifically address the demands of high cell density fermentation of bacteria, yeasts, and fungi. Robust magnetic overhead drives featuring Rushton-type impellers support agitation rates up to 1200 rpm and provide high performance mass transfer. Eppendorf exclusive integrated cooling baffles enable efficient heat removal for exothermic processes. The rigid-wall industrial stirred-tank design ensures scalability and simplifies technology transfer. The monolayer polymer material mitigates risk and uncertainty with regard to leachables and extractables.

“Product features are nice, but results are better”, explains Ma Sha, PhD, Director of Technical Applications at Eppendorf, Inc. in Enfield, USA. “The BioBLU 3f achieved over 300 mmol O2/L/h OTR and outstanding 200 OD600 in E. coli fermentation. Our recent studies prove the excellent comparability of results obtained using the Eppendorf glass and BioBLU f Single-Use Vessels, respectively.”

BioBLU 3f single-use bioreactors are designed as drop-in replacements for existing autoclavable fermentation vessels. They can be operated with the Eppendorf bioprocess control stations BioFlo® 115, BioFlo 310 and BioFlo 320.

The new BioBLU 3f Single-Use Vessel will be presented amongst others at this year’s SIMB Annual Meeting in New Orleans, USA from July 24 – 28. Interested visitors can learn more about the Eppendorf single-use bioreactor solutions for microbial applications at the Eppendorf booth and on www.eppendorf.com/BioBLUf.

BioBLU® 0.3 Single-Use Vessels

Eppendorf

As the smallest vessel of the Eppendorf single-use bioreactor family the BioBLU 0.3 is ideally suited for use as a scale-down model. Designed for use with the DASbox Mini Bioreactor System it is able to accelerate process development in biopharmaceutical and biotechnological applications using cell cultures and microorganisms.All critical process parameters can be precisely controlled and users benefit from easy handling, minimized set-up time and the elimination of cleaning and sterilization steps. The unique liquid-free exhaust gas condenser reduces negative effects caused by change of medium osmolarity and efficiently prevents blocking of the exhaust gas filter.BioBLU® 0.3 Single-Use Vessels Features: Fully instrumented single-use mini bioreactors for accelerated process development in cell culture and microbial applications. Excellent scalability and reproducibility. Small working volumes starting at 65 mL save on cell material, media and supplements. Used with the DASbox mini bioreactor system for parallel set-up of 4, 8, 12 or more bioreactors. Liquid-free exhaust condenser with easy handling by automatic click in activation and click out deactivation mode. Magnet-coupled overhead drive reduces the risk of contamination, up- or downflow. Headplate with 2x PG 13.5 ports, 4x integrated long dip tubes, 2x integrated short dip tubes, one thermowell, DO sensor port with permeable membrane. Operating with industry standard probes for precise measurement and control of temperature, pH, DO. Vessels are delivered pre-sterilized by ß-irradiation to dose exceeding 15 kGy (DIN EN ISO 11137-2:2007). All wetted materials are USP Class VI certified. Applications: Process development in cell culture and microbiology Design of Experiments (DoE) Media optimization Clone & cell line screening, strain characterization

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