A Beginner’s Guide to Bioprocess Modes

22 May 2019

In this application note, Eppendorf explains the differences between batch, fed-batch, and continuous fermentation and how these influence culture growth, using E. coli fermentation processes as an example.

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Innova® S44i
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Innova S44i is a large capacity, stackable shaker with temperature control functionality. It is designed to provide maximum flask and weight capacity at a compact footprint. Eppendorf X-Drive with software-guided counterbalance adjustment supports smooth shaking of uneven and heavy loads at high speeds. All Innova S44i in a triple stack can be operated at maximum speed. The Innova S44i supports the integration of the shaker in regulated labs with its easy-to-use touchscreen user interface with user management capability, USB ports to download operational data and Ethernet for addition to customer network. Service products including installation and operation qualification allow seamless validation of the instrument. Features Highest flask capacity in a smaller footprint – grow more cells in less space The new proprietary Eppendorf X-Drive with semi-automatic counterbalance technology provides smooth and uniform agitation High temperature uniformity across chamber for reproducible results Easily reach all your samples using the slide-out platform Glide-up doors for convenient and effortless access Optimize your space with double- and triple-stacked configurations New touchscreen user interface provides easy operation. Alarm status, event log, and user information is just one touch away. Create multi-step programs for automated control of the shaker Photosynthetic LED light bank for plant and algae growth Wide range of accessories and platforms to meet your every growing need Applications Grow bacteria, yeast, fungus, cyanobacteria and algae Plasmid dNA production and protein expression Process and media development Media screening and optimization Seed cultures for bioprocess scale-up Biofuel research Food quality testing
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Eppendorf BioFlo® 320
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The BioFlo 320 seamlessly blends beauty and utility in one all-inclusive package. A new industrial design, flexibility between interchangeable autoclavable and single-use vessels, intelligent sensors, Ethernet connectivity, and a new software package are only a few of the features that set it apart from the competition
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Eppendorf BioBLU® Single-use Vessels
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Eppendorf BioBLU Single-use Vessels combine all the advantages of single-use technology with the trusted performance and scalability of a stirred-tank design.
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Centrifuge 5810 / 5810 R
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Eppendorf Centrifuge 5810/ 5810 R with its renowned quality and reliability offers you the most cost efficient solution for your medium to high-throughput applications—now and in the future.The Centrifuge 5810/ 5810 R is designed to operate at low noise levels for a relaxing work environment. It features new aerosol-tight Eppendorf QuickLock® caps and lids as well as a low profile for easy loading and unloading of samples. The refrigerated model has unique features such as ECO shut-off function and dynamic compressor control for optimized cooling performance and energy savings.Centrifuge 5810/5810 R is a workhorse for medium to high-throughput laboratories. It combines extraordinary versatility and capacity for both tubes and plates with an extraordinary compact footprint. With the new rotor generation the capacity of Centrifuge 5810/ 5810 R increases to max. 4 x 750 mL (or max. 28 x 50 mL / 56 x 15 mL). Considering its small footprint, Centrifuge 5810/5810 R is the most compact 3 liter centrifuge currently available in the market.Features: Swing-bucket rotors and adapters accommodate tubes and bottles from 0.2 mL to 750 mL Plate rotors for centrifugation of all types of MTP, PCR, cell culture, or deepwell plates Fixed-angle rotors for high-speed molecular biology applications in tubes from 0.2 mL to 85 mL High centrifugation speed of up to 20,913 x g (14,000 rpm) Aerosol-tight Eppendorf QuickLock® caps and lids for easy, one-hand operation Centrifuge lid with soft-touch lid closure Low access height of 29 cm for easy loading and unloading of samples Quiet operation to improve your work environment Compact footprint saves valuable bench space Automatic rotor recognition and imbalance detection for maximum operational safety Special features for Centrifuge 5810 R: Temperature range from -9 °C to 40 °C FastTemp function for fast pre-cooling Standby cooling function holds temperature when centrifuge is not in use ECO shut-off engages after 8 hours of non-use to reduce energy consumption and to extend compressor life Dynamic compressor control (DCC) technology for optimized cooling performance
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Micro Centrifuge MiniSpin® plus
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The mystery has been solved: Real-life personal centrifuges of an out-of-this-world quality really do exist. Eppendorf, the world's number one for microcentrifuges, has opened up a new dimension for personal centrifuges, in the form of MiniSpin® and MiniSpin plus®. With a futuristic exterior home to safe, reliable and durable technology, Eppendorf has managed to fit the tried and tested standards of larger models into the smallest space possible. This is borne out by MiniSpin's unique low running noise. Both personal centrifuges are high-performance, easy-to-operate 12-position models which have been designed especially for modern-day training and industrial research purposes. In other words, what you have here are not sensitive "beta versions", but professional personal devices that can call themselves "centrifuges" without any fear of contradiction. In detail: The maintenance-free drive of MiniSpin accelerates up to twelve 1.5/2.0 ml Eppendorf Tubes® to a maximum speed of 13,400 rpm (rcf: 12,100 x g) in the blink of an eye - and so quietly that you'll hardly know it's there. With its refined black rotor, MiniSpin plus can top even that, reaching a maximum speed of 14,500 rpm (rcf: 14,000 x g). As you would expect, MiniSpin and MiniSpin plus offer typical user comfort à la Eppendorf, including automatic lid release, an easily comprehensible display for intuitive operation and a separate short-spin key for immediate reduced-time runs. In the short-spin mode of MiniSpin plus, users can choose their own speed and set the built-in timer to 99 minutes or "continuous operation". The unique performance spectrum in this centrifuge category is a galaxy of its own: At long last, it is now possible to process samples rapidly. Routine runs, such as spin column preparations can be carried out at your own personalized workstation, with a minimum of time and effort. MiniSpin® and MiniSpin® plus: A new dimension and an out-of-this-world design: these new personal centrifuges are head and shoulders above the competition. In fact, they're from a different world - a world called Eppendorf.
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Bioprocessing / FermentationEscherichia coliBioprocessing

Centrifuge 5810 / 5810 R

Eppendorf

Eppendorf Centrifuge 5810/ 5810 R with its renowned quality and reliability offers you the most cost efficient solution for your medium to high-throughput applications—now and in the future.The Centrifuge 5810/ 5810 R is designed to operate at low noise levels for a relaxing work environment. It features new aerosol-tight Eppendorf QuickLock® caps and lids as well as a low profile for easy loading and unloading of samples. The refrigerated model has unique features such as ECO shut-off function and dynamic compressor control for optimized cooling performance and energy savings.Centrifuge 5810/5810 R is a workhorse for medium to high-throughput laboratories. It combines extraordinary versatility and capacity for both tubes and plates with an extraordinary compact footprint. With the new rotor generation the capacity of Centrifuge 5810/ 5810 R increases to max. 4 x 750 mL (or max. 28 x 50 mL / 56 x 15 mL). Considering its small footprint, Centrifuge 5810/5810 R is the most compact 3 liter centrifuge currently available in the market.Features: Swing-bucket rotors and adapters accommodate tubes and bottles from 0.2 mL to 750 mL Plate rotors for centrifugation of all types of MTP, PCR, cell culture, or deepwell plates Fixed-angle rotors for high-speed molecular biology applications in tubes from 0.2 mL to 85 mL High centrifugation speed of up to 20,913 x g (14,000 rpm) Aerosol-tight Eppendorf QuickLock® caps and lids for easy, one-hand operation Centrifuge lid with soft-touch lid closure Low access height of 29 cm for easy loading and unloading of samples Quiet operation to improve your work environment Compact footprint saves valuable bench space Automatic rotor recognition and imbalance detection for maximum operational safety Special features for Centrifuge 5810 R: Temperature range from -9 °C to 40 °C FastTemp function for fast pre-cooling Standby cooling function holds temperature when centrifuge is not in use ECO shut-off engages after 8 hours of non-use to reduce energy consumption and to extend compressor life Dynamic compressor control (DCC) technology for optimized cooling performance

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A Beginner’s Guide to Bioprocess Modes

Related products

Innova® S44i

Eppendorf BioFlo® 320

Eppendorf BioBLU® Single-use Vessels

Centrifuge 5810 / 5810 R

Micro Centrifuge MiniSpin® plus

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Innova® S44i

Eppendorf BioFlo® 320

Eppendorf BioBLU® Single-use Vessels

Centrifuge 5810 / 5810 R

Micro Centrifuge MiniSpin® plus