DynaPro™ Zetastar™ Dynamic, Electrophoretic, and Static Light Scattering Detector
Walk-up or automated measurements of dynamic, static and electrophoretic light scattering.
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Multi-measurement capabilities in one instrument
The DynaPro ™ ZetaStar combines dynamic, electrophoretic and static light scattering to characterize nanoparticle suspensions and macromolecular solutions. It is simple to use, but a powerful tool that quickly delivers size and polydispersity, zeta potential, particle concentration, molar mass, and turbidity/opalescence for molecules that range in size from angstroms to microns. Analyze and quantify viral vectors, vesicles, lipid nanoparticles, inorganic nanoparticles, nanoemulsions, polymers, peptides, proteins, nucleic acids, and more.
Automated nanoparticle characterization
The DynaPro™ ZetaStar Instrument stands apart from other light scattering devices with its unique ability to fully automate size and zeta potential measurements. When paired to an HPLC autosampler, achieve precise, unattended analysis on up to 96 samples in a single run—saving time and reducing variability.
Walk-up operation with DYNAMICS Touch™
Designed for casual and non-expert users, DYNAMICS Touch is an intuitive—yet capable—multi-measurement app that resides right on the ZetaStar. Via the touch screen display, DYNAMICS Touch guides measurement setup and data collection. Data quality indicators provide confidence in the results as well as insight to help improve future measurements. Results are instantly displayed on the ZetaStar, while data files and concise PDF reports can be stored on the instrument, transferred via the network or downloaded to a flash drive. You can even send the report to your phone!
Full-featured analyses with DYNAMICS™
DYNAMICS is our powerful PC-based, comprehensive software package for collecting, analyzing, and reporting dynamic, static and electrophoretic light scattering data. It performs advanced analyses and compares multiple data files to identify trends and compile statistics. DYNAMICS can control the ZetaStar and acquire data directly, run a series of automated experiments with an autosampler, or read in DYNAMICS Touch data files.
DYNAMICS is offered with a 21 CFR Part 11 compliant Security Pack option, enabling the use of the ZetaStar in a GMP environment for quality control or at-line process monitoring.
More than just your typical zeta potential detector
Unique among zeta potential detectors, the ZetaStar offers sensitive and robust DLS, ELS, and SLS measurements both in cuvettes and in automation using a flow cell.
- DLS/ELS: The ZetaStar performs simultaneous dynamic and electrophoretic light scattering (DLS/ELS) to determine size, polydispersity and zeta potential.
- DLS/SLS: Simultaneous dynamic and static light scattering (DLS/SLS) enables size, polydispersity, particle concentration, molar mass and/or turbidity measurements in as little as 2 µL.
- Automation: The ZetaStar can be paired with an HPLC autosampler and pump to automate measurements of zeta potential and size for up to -96 samples at a time.
- Convenience: DLS/ELS and DLS/SLS can also be carried out in disposable cells to eliminate cuvette cleaning operations and potential sample carryover.
- Low volume: DLS/ELS can be performed with as little as 65 µL of solution, and DLS/SLS with as little as 2 µL.
- Speed: DLS/ELS measurements can take just 15 seconds, and DLS/SLS measurements even less.
- High salt: ZetaStar can measure zeta potential in solutions representative of physiological conditions or formulation buffers with high sensitivity and no damage to fragile biological samples .
Quick and easy: Coupled with the user-friendly on-board app, DYNAMICS Touch™, quick and robust results are just a few clicks away.
Maximum power: Paired with the full power of PC-based DYNAMICS™, the ZetaStar can determine extended solution properties including:
- Colloidal stability: second virial coefficient (A2 or B22) and diffusion interaction parameter (kD), turbidity or opalescence
- Thermal stability: temperature of protein melting or aggregation onset (Tm, Tonset, Tagg)
- Accelerate degradation kinetics: the evolution of size and molar mass with time at elevated temperature.
Minimum downtime: Constructed of a set of field-replaceable modules with no need for optical alignment, the ZetaStar need never leave your lab for maintenance. A Wyatt technician can make most repairs on-site.
ZetaStar features an intuitive onboard app, DYNAMICS Touch, which enables anyone to walk up to the instrument, insert a cuvette with sample and be guided through a measurement.
Automated, high-precision liposome zeta potential measurements at high and low salt concentrations
Zeta potential is used as a key metric for colloidal suspension stability for a wide range of applications. However, the general usefulness of zeta potential measurements has been held back by instruments limited to time-consuming, low-precision measurements within a narrow and often unsuitable ionic strength range.
In this application note, Wyatt Technology demonstrates how the DynaPro™ ZetaStar™ instrument allows scientists to go beyond these limitations, delivering automated measurements of biomolecule and nanoparticle zeta potential, size, and polydispersity at both low and high ionic strengths with excellent precision and accuracy.
A biopharmaceutical workflow powered by Wyatt Technology
Reliable biophysical characterization is a cornerstone of biologics development, playing a critical role at every stage – from selecting promising drug candidates to optimizing formulations and ensuring consistent manufacturing processes. Explore a comprehensive guide on how to deliver biopharmaceutical solutions, beginning with light scattering technology.
Particle size analysis by DLS for optimization of protein complexes for measurement by small-angle neutron scattering
Explore how dynamic light scattering (DLS) can be used to ensure monodispersity in protein samples for small-angle neutron scattering (SANS) studies. Discover how researchers using the DynaPro™ Plate Reader, detected protein aggregation that was undetectable by SDS-PAGE and UV spectroscopy. This method enhances sample quality assessment, ensuring reliable SANS measurements for studying Type I bacterial restriction-modification systems.
Measuring size, concentration, and zeta potential of LNPs with the DynaPro ZetaStar
Microfluidic formulation of RNA-containing lipid nanoparticles (LNP-RNA) is a common means of developing and producing this important non-viral modality of gene delivery. A key challenge during formulation is the selection of the specific lipids and formulation conditions to achieve the desired particle sizes and surface potential. Discover how by using the DynaPro™ ZetaStar™ instrument, researchers can rapidly characterize size, polydispersity, and zeta potential, comparing outcomes from two LNP formulation techniques to optimize efficiency and performance.
DynaPro ZetaStar: Automation, speed, and simplicity for nanoparticle characterization
In this video, Wyatt Technology demonstrates the power of automated nanoparticle characterization with the DynaPro™ ZetaStar™. Combining dynamic, electrophoretic, and static light scattering in one advanced system, it delivers precise measurements of size, polydispersity, zeta potential, particle concentration, molar mass, and turbidity, all in a single instrument. From viral vectors and lipid nanoparticles to proteins, polymers, and nucleic acids, the ZetaStar analyzes molecules ranging from angstroms to microns with speed and confidence.
Collaboration drives innovation in biotherapeutics
Waters and Wyatt Technology join forces to help accelerate the therapeutic pipeline and allow biotherapeutics to reach patients quicker
