CORTECS® Columns

As the legalization of cannabis for both medicinal and recreational use continues to advance, the need for simple, reliable analytical methods for the analysis of these products is desired by many parties. In this application note, Waters present the modification of a simple isocratic HPLC separation of 16 cannabinoids to a separation under ULC conditions.

In this application note, Waters presents the modification of a simple isocratic HPLC separation of 16 cannabinoids to a separation under UPLC conditions.

In this application note, a comparison was performed between Oasis PRiME HLB SPE, SLE and LLE in both plasma and urine matrices for bioanalysis and forensic toxicology. In plasma, 22 comonly analyzed pharmaceuticals, steroids, and drugs of abuse were extracted using the three aforementioned methods and the results were compared. In urine, 23 drugs of abuse representing opioids, stimulants, benzodiazepines, and synthetic cannabinoid metabolites were tested for forensic toxicology analysis.

Structurally similar compounds can be difficult to separate, making the development of rapid chromatographic methods for them very challenging. Changing the column stationary phase is one important tool to provide increased resolution. using smaller and/or solid-core particles can improve efficiency. However, another source of increased resolution can be the selectivity difference inherent in stationary phases. While it's most common to vary the bonded phase chemistry, selectivity differences may also be achieved by varying the base particles.

Beta-adrenergic receptor agonists are synthetic compounds that mimic some of the effects of naturally-occuring compounds by binding to beta-receptors on the surface of cells within the muscle, fat, and other tissues of animals. Some beta-agonists are used in human medicine for the treatment of conditions such as asthma, but they have also been used in livestock production to enhance growth and alter body composition.

This application note describes a rapid and simplified solid phase extraction protocol and LC-MS/MS method for the analysis of urinary benzodiazepines and metabolites for forensics toxicology use.

CoQ10 is a unique hydrophobic molecule that is a key component in ATP synthesis. In this application note, the efficient extraction and analysis of CoQ10 from plasma samples is detailed.

A chromatographic peptide mapping method must provide adequate resolution and recovery of a broad set of peptides if it is to be used in the quality analysis of a biotherapeutic protein. In this application note, a range of reversed phase columns by Waters are analysed for their applicability to peptide mapping, demonstrating high peak capacity separations.

This study demonstrates that in the analysis of complex samples, such as plant extracts, the use of mass detection greatly reduces the interferences from co-eluting compounds. Also, time-consuming troubleshooting for out of specification results are reduced, which will in turn improve overall lab productivity and analytical data quality.

The requirement to analyse drugs at low levels in oral fluid has become an important requirement for many forensic laboratories around the world. Cannabis is one of the most widely used illicit substances and analysis of cTHC in saliva can indicate active cannabis consumption. This application note demonstrates how the Xevo TQ-S can be applied with the right sensitivity for such analyses.

Michael Yelle, Waters Technologies, introduces the new CORTECS™ Columns, a new family of 1.6 micron solid-core UltraPerformance LC® Columns that aim to set a new standard in LC column performance. Interview filmed by SelectScience at HPLC 2013.

Company also spotlights how its glycan analysis kit has saved biopharmaceutical industry nearly four years of lab work

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