BioSep™ Aqueous GFC/SEC Columns
Economical, high efficiency columns to purify and/or characterize proteins, peptides, and other biomolecules including antibodies, aggregates, and PEGylated proteinsHigh performance: Analytical and preparative BioSep columns offer high resolution, maximum efficiency, and exceptional peak asymmetryImproved recovery: Extremely inert media offers advantages for accurte quantitationFlexible method development: 3 phases options (20…
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Protein
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Review Date: 28 Jul 2022 | Phenomenex Inc
Economical, high efficiency columns to purify and/or characterize proteins, peptides, and other biomolecules including antibodies, aggregates, and PEGylated proteins
- High performance: Analytical and preparative BioSep columns offer high resolution, maximum efficiency, and exceptional peak asymmetry
- Improved recovery: Extremely inert media offers advantages for accurte quantitation
- Flexible method development: 3 phases options (2000, 3000, 4000) to separate samples of varying molecular weight ranges
Phenomenex offers support services worldwide through PhenoLogix, an in house analytical support laboratory. The team provides full support for labs needing method development and optimization support for new size excluion methods, as well as transferring current methods from other GFC media to BioSep-SEC-S phases.
LC-MS/MS Environmental Applications
The world is changing and we need to widen our Environmental Scope. Although analytical techniques and established regulated methods are not easily changed; more complex targets, larger lists, and a variety of sample matrices create a daily challenge for environmental chemists. By using the latest technologies and techniques in HPLC/ UHPLC and Sample Preparation, laboratories and chemists are able to develop better approaches to make their work Faster, Cheaper and Easier. Phenomenex is committed to supporting the industry through a comprehensive product portfolio of Sample Preparation, HPLC/UHPLC, and LC-MS, columns and accessories, along with application and method development services to meet industry needs.
Analyzing Post-Translational Modifications of Intact IgG Using Aeris™ WIDEPORE Core-Shell HPLC/UHPLC and BioSep™ GFC/SEC Columns
The ever growing interest in protein therapeutics has found researchers looking for improved solutions for identifying posttranslational modifications of proteins. For the analysis of large, intact proteins like recombinant IgG therapeutics, Phenomenex offers two bioseparation product lines (Aeris and BioSep) for improved solutions in identifying low-level post-translational modifications. Aeris WIDEPORE is a reversed phase core-shell HPLC/UHPLC column that features improved resolution and recovery versus traditional fully porous 300 Å media. BioSep columns offer high performance gel filtration separations that offer improved resolution and inertness versus other aqueous GFC columns. This application note features examples of the use of each column for analyzing recombinant IgG.
A Simple Method for Analyzing Aggregates of EPO (Erythropoietin) Using a BioSep™-SEC-s2000 GFC Column
In this application note by Phenomenex, a rugged yet simple method is presented for separating EPO and its dimer. The difference in the amounts of aggregate present in both fresh and long-term stored EPO samples is determined using the BioSep-SEC-s2000 column. Recombinant EPO is approximately 30 kDa molecular weight in its glycosylated form (approximately 18 kDa for proteins), and any dimer of EPO would be expected to be around 60 kDa in size. A BioSep-SEC-s2000 series column was used for EPO separation by gel filtration chromatography, as it provides a large separation window for low molecular weight proteins.
