Analyzing Post-Translational Modifications of Intact IgG Using Aeris™ WIDEPORE Core-Shell HPLC/UHPLC and BioSep™ GFC/SEC Columns

  Core-Shell Particles Precision Engineered for Protein and Peptide Separations.   Core-shell particle technology provides striking increases in peak capacity and resolution at lower backpressure, giving chromatographers the ability to achieve ultra-high performance on ANY system, HPLC or UHPLC. A uniform porous silica layer is grown around a solid, spherical silica core, providing effective retention and selectivity with improved resolution, speed, and recovery. Next, optimizing the pore size and shell thickness for intact proteins or smaller peptide fragments provides well-defined depth penetration of biomolecules leading to maximum separation power. Column Specifications: Phase: PEPTIDE XB-C18, WIDEPORE C4, WIDEPORE XB-C18, WIDEPORE XB-C8 Particle Size: 1.7, 2.6, 3.6, 5.0 µm Pore Size: 200, 100 Å Length: 50, 100, 150, 250 mm ID: 2.1, 3.0, 4.6, 10.0, 21.2 PEPTIDE XB-C18 Synthetic peptide impurity analysis Peptide mapping Identifying protein modifications - Glycosylation - Substitution - Truncation Analyzing post-translational modifications - Deamidation - Oxidation - Deletions WIDEPORE XB-C18 Proteins Hydrophilic proteins PEGylated proteins High temperature separations Alternative selectivity for peptide mapping WIDEPORE XB-C8 Large proteins Moderately hydrophobic proteins Monoclonal antibodies Glycosylated proteins High temperature separations WIDEPORE C4 Very large proteins Very hydrophobic proteins Membrane proteins Least retentive

Economical, high efficiency columns to purify and/or characterize proteins, peptides, and other biomolecules including antibodies, aggregates, and PEGylated proteinsHigh performance: Analytical and preparative BioSep columns offer high resolution, maximum efficiency, and exceptional peak asymmetryImproved recovery: Extremely inert media offers advantages for accurte quantitationFlexible method development: 3 phases options (2000, 3000, 4000) to separate samples of varying molecular weight rangesPhenomenex offers support services worldwide through PhenoLogix, an in house analytical support laboratory. The team provides full support for labs needing method development and optimization support for new size excluion methods, as well as transferring current methods from other GFC media to BioSep-SEC-S phases.