Big Tuna
The ultimate automated buffer exchange solution.
The ultimate automated buffer exchange solution
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Cut loose from manual buffer exchange
Buffer exchange is the time consuming, hands-on chore you have to deal with before all the things you really want to do. Big Tuna automates the entire tedious process and puts all the control at your fingertips.Exchange one protein, 96 or anything in between. It handles big and small volumes with high precision, so you’ll sail through exchanges without a hitch.
- 1–96 formulations
- 0.1–192 mL sample
- 30 min hands-on time
- Up to 200 mg/mL protein
Meet Big Tuna: Automated, Versatile Buffer Exchange
Buffer exchange with Big Tuna is highly customizable and adaptable, allowing for buffer exchange of up to 96 unique proteins and formulations in a single experiment. Unchained Labs have developed two filter plate formats for this process. The Unfilter 96 and 24 are 10 kDa molecular weight cut-off (MWCO) filtration plates which are designed to withstand 60 psi pressurization during the buffer exchange process. Unfilter 96 can process up to 96 samples ranging from 100-450 µL; Unfilter 24 can process 24 samples ranging from 0.45-8 mL in a single run.
Automated Platform Buffer Screening for Multiple Proteins on Big Tuna
Big Tuna was developed to address gaps in low-volume, high-throughput buffer exchange. Big Tuna uses a pressure-based ultrafiltration/diafiltration (UF/DF) method to remove buffer. During the pressure-based filtration, the plate is gently mixed, ensuring that protein cannot accumulate at the membrane surface, while keeping flow more uniform and faster than dead end filtration methods. Big Tuna automates the buffer exchange process, reduces hands-on time and increases throughput. Big Tuna also enables concentration to a new target after the exchange is complete.
Get the full scoop on your ADC from microliters
Watch this on-demand webinar to learn how to obtain data from mAbs and their conjugates using tools from Unchained Labs
Is protein sample preparation taking too much time in your lab?
Discover how Big Tuna, a fully automated buffer exchange platform, can simplify and accelerate your research, in this on-demand webinar
Is protein sample preparation taking too much time in your lab?
Join this new webinar to learn how Big Tuna, a fully automated buffer exchange platform, can simplify and accelerate your research
Don’t Risk Protein Quality: Your Questions about Automating Buffer Exchange Answered
Learn about a new solution to automate buffer exchange for proteins and biologics with these highlights from our fascinating webinar – now available on demand
