Agilent 1290 Infinity II LC System

Robust and reliable screening methods for pesticide analysis in food matrices are a continuous point of challenge with the growing number of matrix samples, varying detection limits, and a continuously expanding list of emerging contaminants. Data-independent acquisition (DIA) methods allow users to simultaneously screen for targets of interest while also evaluating valuable information on unknown compounds. Agilent Technologies demonstrates a DIA methodology for the screening of pesticides in a complex matrix with the Agilent Revident Q-TOF LC/MS. Plus, discover how intelligent reflex automated targeted reinjection has been used for the acquisition of compounds screened as present or questionable.

Residues of pesticides remaining in or on commodities such as fruits, vegetables, or cereals can cause adverse health effects as well as environmental concerns. In Europe, pesticide testing laboratories adhere to the SANTE 11312/2021 guideline, which ensures a consistent approach for controlling maximum residue levels (MRLs) that are legally permitted in food or animal feed, and requires an elaborate analysis. Agilent highlights a workflow that will help streamline routine pesticide analysis, analyzing over 1,000 pesticide residues in tomato using a single QuEChERS extraction for sample preparation.

From nonstick cookware to fire-fighting foam, per- and polyfluoroalkyl substances (PFAS) have been some of the most widely used chemicals in industry and manufacture for the past 70+ years. These human-made chemicals do not break down easily and thus are extremely pervasive in the environment and the body. In this application note, Agilent explores the basics of PFAS analysis and the regulatory framework for PFAS analysis. Both laboratories new to PFAS analysis and experts alike will learn best practices for PFAS analysis and how to future-proof their lab for this ever-evolving area of environmental analysis.

In this application note, Agilent describes a method for the separation and detection of 28 per- and polyfluoroalkyl substances (PFAS) in water samples. The method uses an Agilent 1290 Infinity II LC coupled to an Agilent 6470A triple quadrupole LC/MS system with Agilent MassHunter workstation software. All the PFASs included in the ASTM 7979 method are analyzed, and the same sample preparation protocol is used. Water samples of 5 mL are diluted with an equal volume of methanol and injected directly for a reporting limit of 10 parts per trillion (ppt, ng/L) or lower for most of the compounds.

In this application note, Agilent addresses the challenge of detecting trace levels of per- and polyfluoroalkyl substances (PFAS) in wastewater due to their stability and bioaccumulation potential. Agilent presents a comprehensive workflow based on EPA draft Method 1633, involving solid-phase extraction (SPE) and clean-up steps, followed by LC-MS/MS analysis and automated reporting. This methodology provides a reliable and robust solution for targeted PFAS analysis in complex matrices, overcoming interference from environmental matrices and background contaminants.

In this application note, Agilent highlights the growing concern over the persistence and toxicity of per- and polyfluorinated substances (PFAS). While most attention focuses on a limited number of PFAS, targeted analysis often covers only a fraction of the total compounds. Uncommonly measured or unknown PFAS are increasing due to manufacturing alternatives, necessitating non-targeted analysis. Agilent introduces FluoroMatch 3.0 to address this gap, presenting its new developments and application to dried blood spots, aiming to enhance PFAS measurement coverage and understand their implications on human health more comprehensively.

The effect of various ion pairing reagents (amines and acidic counterions) on the LC-UV and LC-MS analysis of oligonucleotides (ONs) has been studied. The type of amine and concentration of the counterion play a significant role in the retention and resolution of the ONs and strongly affect the MS data.

In this application note, Agilent Technologies demonstrates that there are numerous variables available for optimization of the analytical conditions for ONs. The analyses were carried out on an Agilent 1290 Infinity II LC System with UV detection by DAD and an Agilent 6530 LC/Q-TOF.

In this application compendium from Agilent, discover a range of glycan analysis solutions to optimize your workflows.

The presence of per- and polyfluoroalkyl substances (PFAS) in drinking water is of emerging concern globally due to their widespread usage, environmental persistence, and bio-accumulative tendency. This application note includes a comprehensive step-by-step workflow guide, ready to run acquisition and quantitation methods, and detailed ordering information to facilitate implementation of a new PFAS analysis workflow.

This application note describes the use of the Agilent AdvanceBio Sialic Acid profiling and quantitation kit to profile and perform absolute quantitation of sialic acids present in biotherapeutic glycoproteins as well as the NISTmAb.

Per- and polyfluorinated substances (PFAS) are a group of human-made chemicals used in various industries, known for their persistence in the environment and potential health risks. In this webinar, Agilent discusses the use of both targeted and untargeted workflows, emphasizing a comprehensive approach to understanding PFAS. Experts share information on the latest techniques and technologies, enabling researchers and professionals to gain deeper insights into PFAS and address environmental and health concerns related to these substances.

In this webinar, Agilent presents a glimpse into the future of PFAS labs, focusing on optimized workflows, enhanced efficiency, artificial intelligence, and increased throughput. Unlike traditional discussions about methods and regulations, this session explores a forward-looking lab environment. It delves into automated sample collection and preparation, employing smart, self-driving analytical instruments capable of automated maintenance feedback, minimizing manual data review through intuitive software. The future PFAS lab prioritizes user experience, compliance, and seamless data transfer, creating a streamlined and productive analytical space.

In this video, Agilent Technologies emphasizes the importance of sustainability in life science research, and introduces its adjuvant chromatography instruments, which are built sustainably and designed to be resource efficient. Agilent Technologies has collaborated with My Green Lab to achieve excellent results with the ACT label (accountability, consistency, and transparency), a Global Green Accreditation.

See how Chris Elliott, Professor of Food Safety and founder of the Institute for Global Food Security at Queen's University Belfast employs Agilent mass spectrometry to help reliably analyze food fraud.

Discover insights from environmental engineer Michael Thurman, from Colorado University.

Find out how the separation power of Agilent’s 2D-LC solution, together with the ion mobility-capability of the Agilent 6560 Ion Mobility Q-TOF LC/MS, are ideal for the analysis of isobaric compounds, like those commonly found in Chinese medicine, and how the speed and ease of operation means several thousands of compounds can be analyzed in a single run, in one hour.

In this video, learn how Dr Matthias Pursch, of The Dow Chemical Company, uses Agilent's multi-heart-cutting 2D-LC and comprehensive 2D-LC solution for better resolution and quantitation, and the intuitive operation software for faster method development and higher throughput.

We reveal the latest products to earn these coveted awards based on exceptional reviewer feedback

From video interviews with top scientists to our interactive application guide, explore this collection of resources to help hone your technique for optimum results

Gang Chen, lab manager at Simon Fraser University, reveals his contribution to tackling the pandemic and how communication is vital in science

We reveal the cell counters, LC systems and particle size analyzer that have earned one of these coveted awards based on exceptional reviewer feedback

Don't miss upcoming masterclass on how to maximize the lifetime and performance of your LC columns

Company showcases new 1260 Infinity II LC System

Module shortens injection cycles, enlarges sample capacity, lowers carryover