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HPLC: 5 top resources to maximize accurate data generation

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From video interviews with top scientists to our interactive application guide, explore this collection of resources to help hone your technique for optimum results

18 Nov 2021
Rory Shadbolt
Publishing / Media

Editorial article

High-performance liquid chromatography (HPLC) is a process to separate components in a liquid mixture, and due to the flexibility of the technique, it has been adopted in multiple fields for its many applications, including pharmaceutical, analytical, and even clinical laboratories. Today, HPLC is one of the gold standard methodologies for biopharmaceutical processes and you won’t find many labs without an instrument

To help support your work with HPLC, we’ve collated a selection of top HPLC, including our interactive HPLC analysis guide and an extensive How-To-Buy eBook presenting all the key considerations when looking to find the best HPLC technology for your lab’s needs. Plus, watch our video interview with a world-leading expert using HPLC to combat food fraud, hear from scientists using Agilent technology to ensure honey authenticity, and check out a free SelectScience webinar detailing everything you need to know about HPLC columns for biomolecular analysis.

1. Content hub: Experience our interactive HPLC analysis guide

Our new HPLC content hub is a one-stop-shop for the latest HPLC resources

Explore this interactive resource to access a range of chromatographic applications for critical quality attribute (CQA) monitoring within the most common bioanalysis workflows, along with in-depth features, top tips, videos, and other key resources designed to ensure reliable analytical results.

Enter the content hub

2. HTB eBook: Download the HPLC Technology eBook

Our eBook can help you find the best HPLC technology for your lab’s needs

In this new 6th edition buying guide, discover the key factors and application considerations when looking to buy new HPLC technology. Plus, read impartial reviews to gain insights from industry-leading professionals. 

Download the SelectScience How to Buy eBook to find out more about:

  • The principles of HPLC

  • Sample preparation processes

  • Application considerations

  • Selecting the right column

  • The future of HPLC

Download the HPLC eBook

3. Video: Reliable targeted analysis solutions to fight food fraud

Prof. Chris Elliot uses Agilent instrumentation in his fight against food fraud

Watch this video to see how Chris Elliott, Professor of Food Safety and founder of the Institute for Global Food Security at Queen's University Belfast, employs Agilent mass spectroscopy and HPLC to help reliably analyze food fraud.

Watch Chris fight food fraud

4. Video: Robust analysis for ensuring honey authenticity

Lab manager Matt Campion talks about the HPLC instrumentation that is used to verify the authenticity of honey

For over 100 years, Airborne Honey has worked to ensure its honey products are honest, undamaged, and traceable through the analysis of ~2,000 honey samples a year, culminating in a comparative database containing more than 40,000 records. In this video, Matt Campion shares how Airborne’s in-house lab tests for honey authenticity using a range of techniques and new HPLC instrumentation that provides data quality while maintaining high throughput.

Find out how HPLC helps Airborne

5. Webinar: The benefits of dedicated HPLC instrumentation and columns for analysis of biomolecules

Andrew Coffey and Cornelia Vad, both of Agilent Technologies, share insights on column usage in HPLC for the analysis of biomolecules

In this free on-demand webinar, Andrew Coffey and Cornelia Vad, Agilent Technologies, demonstrate how biologics differ from small molecules, and how the InfinityLab Bio LC Solutions from Agilent can help you make an assured start in biopharmaceuticals.

Watch this on-demand webinar

You can find more HPLC content on SelectScience by clicking here >>

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Liquid Handling WorkstationLiquid handling workstations automate the process of sample preparation, improving accuracy and efficiency. These systems are essential for high-throughput applications in biological, chemical, and pharmaceutical research, reducing human error and increasing reproducibility. Workstations come equipped with robotic arms, pipetting tools, and other accessories to streamline lab workflows. Explore liquid handling workstations in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.HPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.LC-MSLiquid Chromatography-Mass Spectrometry (LCMS) is a powerful analytical technique that combines the separation power of liquid chromatography with the detection capabilities of mass spectrometry. It is widely used for qualitative and quantitative analysis of complex mixtures in pharmaceuticals, proteomics, and environmental studies. Browse our peer-reviewed product directory to find the best LCMS systems, compare products, check reviews, and get pricing directly from manufacturers.

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