Evaluation of different ion-pairing reagents for LC-UV and LC-MS analysis of oligonucleotides
24 Feb 2022The effect of various ion pairing reagents (amines and acidic counterions) on the LC-UV and LC-MS analysis of oligonucleotides (ONs) has been studied. The type of amine and concentration of the counterion play a significant role in the retention and resolution of the ONs and strongly affect the MS data.
In this application note, Agilent Technologies demonstrates that there are numerous variables available for optimization of the analytical conditions for ONs. The analyses were carried out on an Agilent 1290 Infinity II LC System with UV detection by DAD and an Agilent 6530 LC/Q-TOF.
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Agilent 1290 Infinity II LC System
Agilent TechnologiesThe 1290 Infinity II LC System is an ultra-high performance LC that has market-leading sample capacity and is designed for the lowest dispersion for the most challenging ultra-HPLC experiments. The 1290 Infinity II LC system is designed to be both a HPLC and high-end UHPLC system in a single flow path, giving you robust and excellent performance no matter what application you are running while seamlessly integrating into your existing laboratory environment.
6530 Q-TOF LC/MS
Agilent TechnologiesRaise your analysis to a new level with the Agilent 6530 Quadrupole Time-of-Flight LC/MS system. The 6530 delivers the power of high-resolution, accurate-mass MS/MS, so you can be fully confident of your data.