Editorial Article: Wondering which DNA probe is right for your assay?

Standardize your assay. Dual-labelled BHQ Probes are traditional probes that contain a 5’ fluorophore and a 3’ Black Hole Quencher dye covalently bound to an oligo. Choose from a broad spectrum of proprietary and common market fluorophores and their corresponding Black Hole Quencher dye to obtain high-quality qPCR data.

BHQplus™ probes

Strengthen your assay with shorter probes specifically designed to enhance specificity and improve mismatch discrimination through the use of modified C and T nucleotides. Reduce assay cost and improve data quality by redesigning MGB probe assays with BHQplus technology.

BHQnova™ probes

Power your assay when longer probes are needed by substantially reducing background interference and increasing the signal-to-noise ratio with double-quenched BHQ probes utilizing the power of both an internal nova quencher and a 3’ BHQ quencher.

Improve assay sensitivity and specificity with Locked Nucleic Acid oligonucleotides (LNA™ oligos). LNAs are modified RNA bases in which the ribose is “locked” with a methylene bridge, connecting the 2' oxygen atom to the 4' carbon atom, fixing it in the C3'-endo conformation.

KASP™ on Demand (KOD)

Choose premier KASP™ on Demand (KOD) service for design and delivery of your KASP assay, plus full validation and optimization of your assay.

KASP™ by Design (KBD)

Choose standard KASP™ by Design (KBD) service for design and delivery of a KASP assay to your SNP or indel of interest. Assay design (primer selection) is carried out using proprietary Kraken™ software system.

Molecular Beacons

A dual-labelled probe that relies on a hairpin conformation to heighten assay specificity. This property allows Beacons to discriminate mismatches as specific as a single nucleotide polymorphism (SNP). Beacons generate fluorescence through hybridization and under non-hydrolytic conditions allowing post application PCR melt curve analyses.

Scorpions™ Primers

A dual-labelled probe that combines a hairpin structure and a PCR primer element in a single oligo, allowing for target detection through a unimolecular mechanism. Mismatched hybrids are less stable than the reformed stem region. Therefore, Scorpions Primers only produce signal when the probe region hybridizes to a perfect-match target sequence within the primer extension product.