Hosted by Kapa Biosystems at AMP2015, Dr. Brian Walker of the Royal Marsden Hospital presents at a work shop entitled "Application of NGS in the diagnostic lab on archival FFPE tissues".
KAPA DNA Library Preparation Kits for Illumina sequencing platforms contain evolved and optimally formulated enzymes that enable the highest overall coverage from the least amount of total sequencing. Kits are optimized to achieve significantly higher library yields and contain KAPA HiFi for high-fidelity, high-efficiency and low-bias library amplification. This ensures the highest library and sequence data quality, particularly from low input and difficult samples such as FFPE and ChIP DNA. Features: Achieve greater molecular complexity Higher yields of adapter-ligated library translates to higher molecular complexity Fewer cycles of amplification are needed, which results in lower PCR duplication rates PCR-free workflows possible from inputs ≥100 ng Reduce amplification bias and improve coverage KAPA HiFi reduces PCR bias Improves coverage uniformity of GC- and AT-rich regions, promoters, low complexity and other challenging regions Create high-quality libraries from challenging samples High-quality whole exome sequencing from libraries prepared using as little as 10 ng human genomic DNA High success rates with 250 ng FFPE or less Routine library construction from ≥100 pg ChIP DNA Automate with pre-validated scripts PerkinElmer Sciclone NGS and NGSx Beckman Coulter Biomek FXp and FX dual-pod Agilent Technologies NGS Workstation Option B Eppendorf epMotion 5075 TMX Applications: Whole Genome Sequencing ChIP Sequencing Whole Exome Sequencing Amplicon Sequencing Targeted Sequencing (Capture) Methyl Sequencing
Versatile, streamlined solution for DNA library preparation for Illumina sequencing.
KAPA Stranded mRNA-Seq Kits includes all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing, utilizing 100 ng – 4 µg of total RNA. KAPA mRNA Capture Beads are included for isolation of poly(A)-tailed RNA. Kits provides precise measurement of strand orientation (>99%), uniform coverage, and high-confidence mapping of alternate transcripts, and are optimized for the improved coverage of GC-rich and low-abundance transcripts. Kits contain KAPA HiFi for high-efficiency and low bias library amplification, as well as KAPA mRNA Capture Beads and a streamlined, “with-bead” protocol. KAPA Stranded RNA-Seq Kits include all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing, but do not contain the KAPA mRNA Capture Beads. Kits can be used to prepare libraries from 10-400 ng of either poly(A)-selected, ribosomally-depleted, or total RNA. Features: Uncover challenging transcripts Improved coverage of GC-rich transcripts Enhanced identification of exonic regions Detect low-abundance transcripts Enables identification of transcripts missed by competitor kits, even with high input High uniformity across varying amounts of sample input Identify more genes Higher percentage of uniquely mapped reads compared to Illumina TruSeq™ Stranded mRNA Sample Prep Kits Lower duplication rates yield better coverage Maintain high coverage uniformity Minimal 5′–3′ bias across transcripts More uniform distribution of reads over each transcript Applications: Gene expression Single nucleotide variation (SNV) discovery Post-transcriptional SNVs Fusion gene identification Targeted transcriptome Whole transcriptome
