KAPA HyperPlus Kits
NGS library preparation. Evolved.
KAPA HyperPlus Workflow
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Library prep
Easy to use
Review Date: 1 Dec 2023 | Kapa Biosystems, Inc.
Efficient and easy to use Illumina library prep kit without need for Covaris
Illumina sequencing
This is an easy to use streamlined kit for preparing Illumina sequencing libraries. One can make libraries in single tube or plates in a little over 2 hours. The chemistry is highly efficient (see Aigrain et al BMC Genomics 2016) allowing for lower input and/or fewer PCR cycles. Enzymatic cleavage appears non-biased.
Review Date: 23 Dec 2016 | Kapa Biosystems, Inc.
The KAPA HyperPlus kit provides a streamlined workflow that includes fragmentation and library preparation in a single tube.
Building on industry-leading library construction efficiencies, this integrated solution combines enzymatic fragmentation, similar in quality to mechanical shearing, with the speed and convenience of tagmentation-based workflows.
Benefits:
- DNA fragmentation and library prep in 2.5 hours
- Flexible DNA sample input from 1 ng – 1 µg
- Reduced bias and maximize sequence coverage
- PCR-free workflows
Features:
Tunable and Reproducible Fragmentation
- Adjust library insert sizes from 150 – 800 bp by varying fragmentation time
- Reproducible insert sizes across a range of GC content and DNA input amounts
Industry Leading Library Yields…
- Routinely achieve conversion rates >80% from ≥ 100 ng input
- Superior performance across a range of DNA input amounts
- High library yields enable PCR-free workflows from as little as 50 ng starting material
…Enable Superior Sequencing Results
- Higher conversion rates results in fewer amplification cycles and lower duplication rates
- Detect low-frequency mutations with high confidence due to greater library diversity and more uniform sequence coverage
Minimal Sequence Coverage Bias
- Lower sequence bias when compared to tagmentation and other enzymatic fragmentation methods
- Equivalent performance to mechanical shearing
- Less bias leads to more uniform sequencing coverage and reduced sequencing costs
Applications:
- Whole Genome Sequencing
- Whole Exome Sequencing
- Targeted Sequencing (custom panels)
- FFPE Sample Sequencing
Improving Data Quality and Turnaround Times for Targeted Next-Generation Sequencing of FFPE DNA using a KAPA HyperPlus/SeqCap EZ workflow
This application note evaluates the KAPA HyperPlus Kit from Kapa Biosystems for enzymatic DNA fragmentation and library construction. Formalin-fixed paraffin-embedded (FFPE) tissue is an important source of DNA for cancer genomic studies and clinical diagnostics. The KAPA HyperPlus Kit with integrated enzymatic fragmentation streamlines the SeqCapTM EZ Target Enrichment workflow, yielding high quality libraries.
Fast Turnaround Times and Improved Data Quality for Microbial Whole-Genome Sequencing with a Novel, Single-Tube Enzymatic Fragmentation
Next-generation whole genome sequencing of microbes demands rapid, robust, and scalable library construction workflows, capable of generating high-quality sequence data across a wide range of genome sizes, complexities and genomic GC content. This application note describes a streamlined library preparation method that results in minimal bias, high uniform coverage, and facilitates de novo assembly of microbial genomes.
Application of NGS in the Diagnostic Lab on Archival FFPE Tissues
Hosted by Kapa Biosystems at AMP2015, Dr. Brian Walker of the Royal Marsden Hospital presents at a work shop entitled "Application of NGS in the diagnostic lab on archival FFPE tissues".
NGS Boot Camp Episode 1: *Objectives of Library Construction*
Learn more about the Theory and Practice of NGS Library Preparation in Dr. Maryke Appel’s 3-part “NGS Boot Camp” series. The first episode, "Objectives of Library Construction", includes topics such as sample types, coverage depth, fragment length, and different library construction strategies. Watch the entire series at www.kapabiosystems.com/webinars.
