Cell Signaling Assays Products & Reviews

The Griess Reagent System measures nitrite (NO2–), which is one of two primary stable and nonvolatile breakdown products of nitric oxide (NO). Nitric oxide is an important physiological messenger and effector molecule in many biological systems, including immunological, neuronal and cardiovascular tissues. This assay relies on a diazotization reaction that was originally described by Griess in 1879. Through the years, many mod…

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase.   The luminescent signal positively correlates with kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary scre…

Measure Kinase-Ligand Affinity in Live Cells The NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay measures compound binding at select target kinases in intact cells. The assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET™ TE Assay measures the apparent affinity of test compounds by competitive displacement of the NanoBRET™ tra…

Live-cell analysis system

High-throughput live-cell analysis system

This HTRF kit detects changes of cAMP accumulation in response to Gs coupled GPCR activation or inhibition.

Cisbio offers a line of three ready-to-use assays for measuring cAMP using HTRF® technology. Each kit is optimized for detecting a specific range of cAMP concentrations to allow fine tuning of assay sensitivity. These kits enable high quality, cell-based assay screening in 96-, 384- or 1536-well formats.

R&D Proteome Profiler Antibody Arrays R&D Proteome Profiler Arrays allow for the measurement of multiple proteins in a single sample. These arrays require no specialized equipment, and eliminate the need for multiple immunoprecipitation/Western blot experiments. R&D Array kits contain buffers, detection antibodies, and membranes spotted in duplicate with capture antibodies carefully selected for their specificity. R&D Arrays…

ARRALYZE Glass Nanowell Arrays feature precisely engineered nanowells in borosilicate glass, created with our patented LIDE technology. Each array contains up to 897 individual wells (0.4 × 0.4 × 0.225mm) with dimpled bottoms for optimal single-cell isolation and analysis.

Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

The Transcreener ® ADP² TR-FRET Assay produces a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) signal that is less sensitive to interference from fluorescent compounds than other detection modes.

Cost effective high throughput screen for anti-cancer compounds. Basic research to measure compound IC50s and specificity for tubulin. Screening proteins for effects on tubulin polymerization activity. Teaching aid for undergraduate/graduate class in pharmacology. This assay is an economical one step procedure for determining the effects of drugs or proteins on tubulin polymerization. It is an adaptation of an assay o…

Detection of binding partners for 100 different glycans in purified proteins, serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.

Detection of binding partners for 300 different glycans in purified proteins, serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.

RayBio® Lectin Array 95. Detects glycan profiles using 95 lectins. Suitable for all liquid sample types.

RayBio® Lectin Array 70. Detects glycan profiles using 70 lectins. Suitable for all liquid sample types.

ARRALYZE 8 Chamber Slide combines standard slide format with advanced nanowell technology, featuring 8 chambers with 127 precisely etched nanowells (0.4 × 0.4 × 0.225mm) per chamber. The borosilicate glass bottom (0.175μm thick under wells) provides superior optical clarity for high-resolution imaging while maintaining an inert environment. Includes 3 fiducials per chamber for easy navigation and precise cell tracking.

The Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamate in biological samples.

The Glutamine/Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples.

Count on minimal interference from test compounds when screening your compound library using luminescent, ATP-based kinase assays. We offer the ADP-Glo™ Kinase Assay, Kinase Selectivity Profiling Systems and hundreds of Kinase Enzyme Systems. These sensitive, reliable assays are easily scaled to meet your throughput needs and can be used with virtually any kinase and kinase substrate combination.

Multiplate live-cell analysis system

These ready-to-use single positive biomarker controls are the latest and greatest from the Slingshot Team.