New England Biolabs Inc. Drug discovery > Basic Research Products & Reviews

Presently, NEB supplies more than 200 restriction enzymes, over 120 of which are available in recombinant form.

NEBNext® reagents are a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value. Advantages of NEBNext Reagents for RNA Library Pr…

Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…

The NEBNext® DNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expressi…

he NEBNext® Ultra™ DNA Library Prep Kit for Illumina contains enzymes and buffers that are ideal to convert a small amount of DNA input into indexed libraries for next-generation sequencing on the Illumina platform (Illumina, Inc). The workflow of NEBNext Ultra DNA Library Prep Kit for Illumina is very user-friendly and fast with minimal hands-on time.NEBNext® reagents are a series of highly pure reagents that facilitate samp…

NEBNext® dsDNA Fragmentase® generates dsDNA breaks in a time-dependent manner to yield 100–800 bp DNA fragments depending on reaction time. NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl…

The NEBNext® mRNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents…

Restriction enzyme digests are now even easier!The same high quality restriction enzymes that you have come to trust from New England Biolabs are now available in master mix format, including enzyme buffer and loading dye; simply add your DNA and digest for 15 minutes.Available Mixes: AgeI-HF® RE-Mix® OrderAscI RE-Mix® OrderBstEII-HF® RE-Mix® OrderEcoRI-HF® RE-Mix® OrderEcoRV-HF® RE-Mix® OrderKpnI-HF® RE-Mix® Or…

Rapid, sensitive and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences

PvuII has a High Fidelity version PvuII-HF® (NEB #R3151) High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.  HF enzymes also exhibit dramatically reduced star activity.  HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without de…

The Quick Dephosphorylation Kit contains Quick CIP, a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality.

NEBuilder® HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, a…

The New England Biolabs Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for autom…

The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25°C ) Fast - 5 minutes for cohesive or blunt ends. Convenient - ligation performed at room temperature. Flexible - suitable for all common ligation reactions.

The LunaScript® RT SuperMix Kit is optimized for cDNA synthesis in a two-step RT-qPCR workflow.

Purifies up to 100 µg of high quality total RNA from multiple sample types - using only one kit

Taq DNA Polymerase optimized for epigenetics analysis

Quickly and easily purify high quality DNA from PCR and other enzymatic reactions.

Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Assembly and transformation in just under two hours Flexible sequence design (scar-less cloning) No PCR clean-up step required High transformation efficiencies for inserts up to 20 kb Easily adapted for multiple DNA manipulations, including site-directed mutagenesis

The unique workflow of the NEBNext Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.  

Quickly and easily purify DNA from agarose gels with high yields.

For the extraction of HMW DNA from a variety of tissues, bacteria and other samples (yeast, insect, amphibian)

Gibson Assembly® allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility.

Restriction Enzyme