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LunaScript™ RT SuperMix Kit

New England Biolabs Inc.Available: Worldwide

The LunaScript® RT SuperMix Kit is optimized for cDNA synthesis in a two-step RT-qPCR workflow.

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New England Biolabs Inc.

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Average Rating 4.8

|2Scientists have reviewed this product

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Needs minimal effort to understand how to use the product!


Average Rating 5.0

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Super effective and saves so much time!

Review Date: 16 Jul 2021 | New England Biolabs Inc.

Easy to use for cDNA generation and great qPCR results.


Average Rating 4.7

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Reverse transcription of RNA to cDNA

I used purified total RNA from whole mouse brain as my template. Following the protocol as outlined from NEB, I used 1ug of RNA as template for the reverse transcription. The protocol was simple and straight forward, requiring <2 minutes hands-on time and only 15 minutes on a thermocycler. I purified my cDNA using a PCR cleanup kit and analyzed my cDNA on a NanoDrop 2000. I had great 260/280 and 260/230 ratios, with a total recovery of approximately 70% of input RNA. I diluted this cDNA to 10ng/uL, then a 2-fold dilution curve and tested amplification on by qPCR. The ct values were as expected, and the amplification followed the dilutions. Note that I did an on-column DNase digestion during RNA purification and use exon-exon spanning primers in order to negate gDNA amplification. I recommend LunaScript RT SuperMix for generation of single stranded cDNA. Simple to use with good output.

Review Date: 19 Aug 2019 | New England Biolabs Inc.

Make a simpler choice

  • Single-tube SuperMix contains random hexamer and oligo-dT primers, dNTPs, Murine RNase Inhibitor, and Luna® Reverse Transcriptase
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors
  • Combine with Luna qPCR master mixes for robust RT-qPCR results

Experience best-in-class performance

  • Less than 15 minute first-strand cDNA synthesis protocol
  • All Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Illuminate your two-step RT-qPCR

  • Novel, thermostable Luna Reverse Transcriptase improves performance
  • Consistent linearity, sensitivity, and capacity for reliable RNA quantification

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