LunaScript™ RT SuperMix Kit
The LunaScript® RT SuperMix Kit is optimized for cDNA synthesis in a two-step RT-qPCR workflow.
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Needs minimal effort to understand how to use the product!
qPCR
Super effective and saves so much time!
Review Date: 16 Jul 2021 | New England Biolabs Inc.
Easy to use for cDNA generation and great qPCR results.
Reverse transcription of RNA to cDNA
I used purified total RNA from whole mouse brain as my template. Following the protocol as outlined from NEB, I used 1ug of RNA as template for the reverse transcription. The protocol was simple and straight forward, requiring <2 minutes hands-on time and only 15 minutes on a thermocycler. I purified my cDNA using a PCR cleanup kit and analyzed my cDNA on a NanoDrop 2000. I had great 260/280 and 260/230 ratios, with a total recovery of approximately 70% of input RNA. I diluted this cDNA to 10ng/uL, then a 2-fold dilution curve and tested amplification on by qPCR. The ct values were as expected, and the amplification followed the dilutions. Note that I did an on-column DNase digestion during RNA purification and use exon-exon spanning primers in order to negate gDNA amplification. I recommend LunaScript RT SuperMix for generation of single stranded cDNA. Simple to use with good output.
Review Date: 19 Aug 2019 | New England Biolabs Inc.
Make a simpler choice
- Single-tube SuperMix contains random hexamer and oligo-dT primers, dNTPs, Murine RNase Inhibitor, and Luna® Reverse Transcriptase
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
- Combine with Luna qPCR master mixes for robust RT-qPCR results
Experience best-in-class performance
- Less than 15 minute first-strand cDNA synthesis protocol
- All Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
- A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products
Illuminate your two-step RT-qPCR
- Novel, thermostable Luna Reverse Transcriptase improves performance
- Consistent linearity, sensitivity, and capacity for reliable RNA quantification
