Products & ReviewLife Sciences
Quick Dephosphorylation Kit
New England Biolabs Inc.Available: Worldwide
The Quick Dephosphorylation Kit contains Quick CIP, a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.
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Reviews
Amazing results and happy days
Application Area:
To dephosphorylate plasmids post digestions using one cut enzymes.
This is a very easy and quick assay to perform dephosphorylation of single cut plasmids to avoid self-ligation.
Review Date: 28 Mar 2018 | New England Biolabs Inc.
The Quick Dephosphorylation Kit contains Quick CIP, a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.
- Rapid and irreversible heat inactivation eliminates unwanted activity
- Improved storage stability versus native enzyme
- Faster reaction setup (no supplemental additives like zinc required) and shorter incubation time
- Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
- Less enzyme required (high specific activity), resulting in a lower cost per reaction
- No need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )
- Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysis
- Recombinant for purity, consistency and value
