Member since: 2016
Organization: Novartis Pharma AG
Easy to use, great recovery
Application Area: Simple sample purification
"The Vivaspin product family offers a wide range of different volumnes (from 100 ul to 20 ml) and different molecular weight cutoffs (from 3 kDa to 100 kDa). Perfect for the purification of expressed proteins. High recovery achieved!"
Vivaspin™ spin columns are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration.
Spin column protein concentration – how does it work?
Vivaspin sample concentrators cater to scientists working with sample volumes ranging from 100 μL to 20 mL, with a range of MWCO values from Mr 3000 to 100 000.
An upper compartment contains sample and the lower compartment separated by a semipermeable membrane. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.
Tip: To maximize recovery of your sample, select a MWCO at least 50% smaller than the molecular size of the target molecule.