Vivaspin™ protein concentrator spin columns by Cytiva

Manufacturer Cytiva
4.7
/
5.0
  |  1 reviews
Vivaspin™ spin columns are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration.


Vivaspin™ protein concentrator spin columns by Cytiva product image
Vivaspin™ protein concentrator spin columns

Cytiva

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Average Rating: 4.7
1 Scientist has reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2016

  • Organization: Novartis Pharma AG



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Easy to use, great recovery
Rating: 4.7

  • Application Area: Simple sample purification

"The Vivaspin product family offers a wide range of different volumnes (from 100 ul to 20 ml) and different molecular weight cutoffs (from 3 kDa to 100 kDa). Perfect for the purification of expressed proteins. High recovery achieved!"

Review date: 22 Aug 2017 | Vivaspin™ protein concentrator spin columns

Vivaspin™ spin columns are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration.

  • High recovery rates – Up to 30-fold concentration of the sample can be achieved, with recovery of the target molecule typically exceeding 95%.
  • Sample protection – the patented Vivaspin dead-stop technology ensures samples cannot be concentrated to dryness and enables direct concentrate recovery.
  • Ease-of-use – one-step sample concentration in a single tube, for minimal sample handling and reduced sample loss.
  • pH compatibility – for a broad range of pH 1 to 9.
  • High flow rates – and minimal membrane blockage through the vertical polyethersulfone (PES) membrane design.
  • Minimal protein adsorption – thanks to the Vivaspin™ polycarbonate tube design.
  • Download the Vivaspin protein concentrator data file to learn more and to determine the right MWCO (molecular weight cut-off) and volume for your enrichment study.

Spin column protein concentration – how does it work?

Vivaspin sample concentrators cater to scientists working with sample volumes ranging from 100 μL to 20 mL, with a range of MWCO values from Mr 3000 to 100 000.

An upper compartment contains sample and the lower compartment separated by a semipermeable membrane. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.

Tip: To maximize recovery of your sample, select a MWCO at least 50% smaller than the molecular size of the target molecule.