Member since: 2020
Organization: KBI Biopharma
Good results in no time.
Application Area: Formulation buffer screening
"Easy instrument to use with high-throughput screening that makes it easy to analyze multiple samples at a time."
Member since: 2019
Organization: University of Washington
A very useful instrument that has quickly become a standard analytical tool.
Application Area:Characterize protein stability and assembly state, formulation optimization, and QC
"This instrument has quickly become a standard method for a variety of applications including nanoparticle characterization, formulation optimization, and routine protein characterization. With minimal training, unskilled users can become proficient performing SOP methods. Expert users have developed custom methods that have advanced difficult projects. The instrument gives highly reproducible results, although the high sensitivity can make interpretation of some data features difficult. Standard DLS sample preparation considerations apply, and some care in loading samples must be taken. Some people love the software, some people dislike it, but in my opinion, most instrument software is imperfect anyway. Export functionality allows easy interfacing with custom analysis tools. We have had three service calls, all which were resolved quickly. The only downside is the high cost of consumables and some software related issues."
Member since: 2019
Very useful for a quick analysis of proteins
Application Area:Screen proteins for structural biology
"Our results using the Uncle are great. The DLS feature is very useful for seeing large aggregates and the relative size of particles after a very short time. The full spectrum fluorescence is also handy, especially when we are measuring the binding of a compound and want to see a shift in Tm. These two features work very well together. I would recommend it to any lab that requires screening of proteins very quickly (buffer screens, detergent screens etc.)"
Member since: 2016
Gives result in hours, little sample amount, precise, fast.
"Gives result in hours, little sample amount, precise, fast."
Cracking stability using a pile of one-trick, protein-hungry tools is a ton of work. Uncle combines 3 different measurement modes — fluorescence, SLS and DLS. So you can crank out all your data in just a few hours, and use way less protein. All the info you’ll get makes picking the best formulation or protein construct a piece of cake.