Member since: 2020
Organization: Royal Devon and Exeter NHS Foundation Trust
Fantastic kit - has given a diagnosis to a huge number of patients.
Application Area: NHS diagnostics - exome analysis of acutely unwell babies and children in intensive care
"The Twist Human Core Exome kit produces exceptionally high data quality, consistently meeting our threshold cut-off of 98% coverage at 20X depth. Coverage over some of the trickier GC rich/ repetitive regions of the exome is better than competitor products we have previously used, so requires less confirmatory or 'infill' testing by other methods (e.g., Sanger). The introduction of the Universal Adapter System part of the kit has improved our data recovery hugely, giving 10x less undetermined reads. The kits give good value for money for NHS diagnostics and now that kits are modular we have less wastage of excess reagents. Kits are easy to use given the complexity of library preparation and are easily automatable on a variety of liquid handling platforms. Customer service and technical support at Twist is exemplary, with detailed support given for troubleshooting and advice given well ahead of time to any changes in supply of reagents (e.g., due to COVID)."