Member since: 2015
Organization: SBP Medical Discovery Institute
I stop using Trypsin and switch to TrypLE Express to all adherence cell lines.
Application Area:Tissue Culture
"Instead of using Trypsin to detach adherence cells, use TrypLE Express. It is very gentle and can be used for both serum containing and serum free conditions."
Gibco TrypLE reagents are highly purified, recombinant cell-dissociation enzymes that replace porcine trypsin. These reagents are ideal for dissociating attachment-dependent cell lines in both serum-containing and serum-free conditions, and can be directly substituted for trypsin without protocol changes.
TrypLE reagents are:
TrypLE Express reagent
Gibco TrypLE Express reagent proves that superior performance can be affordable. Significant economies of scale make it even easier to replace trypsin with TrypLE Express reagent. For fast, effective cell dissociation that doesn’t harm your cells, use TrypLE Express reagent.
TrypLE Select (1X) reagent
Gibco TrypLE Select (1X) reagent is manufactured on dedicated animal origin–free equipment.
TrypLE Select (10X) reagent
Gibco TrypLE Select (10X) reagent can be used at full strength to quickly and gently dissociate strongly adherent cell types. TrypLE Select (10X) reagent is also manufactured on dedicated animal origin–free equipment.