Member since: 2017
Organization: UNIVERSITY of Naples Federico II
Eco-friendly HRP substrates , very good results,
Application Area: HRP substrates for immunoassays
"I’ve used TMB X-tra substate ready-yo-use to confirm in my previous studies the HRP mimic activity (non enzymatic) and to perform Kinetic experiments."
TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).
TMB X-tra characteristics are: