sbeadex Nucleic Acid Extraction Kits

Molecular testing protocols require reliable and high-quality isolation of genetic material from diverse sample types. In this infographic, LGC Biosearch Technologies explores how its portfolio of products and services can support your molecular testing workflows and shares how to select the best kits for your sample type and downstream requirements.

In this application note, discover how the sbeadexTM tissue kit has been developed to extract high-quality genomic DNA from a wide variety of human and animal tissue materials. Learn how the sbeadex magnetic bead-based extraction technology is adapted for use with the oKtopureTM automated, high-throughput extraction system.

This application note offers an example of how KASP genotyping assays for QTL can be used to survey potential breed-lines for crop development using these publicly available markers. Greater availability of high-throughput genotyping methods has lead to an explosion of single nucleotide polymorphism (SNP) sequence data from plant genomes. This has enabled the discovery of large numbers of potential SNP markers for varietal improvement. The use of KASP genotyping as a cost-effective, efficient tool for introgression of important traits has now been widely published.

The sbeadex® tissue kit has been developed for extraction of genomic DNA from tissue material (muscle, liver, pig ear punch, etc.). The magnetic particle based DNA extraction protocol can be easily automated using a KingFisher 96 (Thermo Fisher Scientific) magnetic particle manipulator. The method described here is a universal tissue protocol which can be used for a wide range of tissue types without adaptation.

The sbeadex® blood kit has been developed for high throughput extraction of genomic DNA from whole blood and buffy coats. The method described here is a blood protocol which can be used for blood samples containing standard anticoagulants (citrate, EDTA, heparin) without adaptation.

Discover an optimized method for the automated extraction of high-quality DNA from bovine tissue samples using the sbeadex™ tissue kit, incorporating a novel two-step binding mechanism, and the oKtopure™ high-throughput extraction platform.

This application note presents a study to evaluate the suitability of the sbeadex® forensic kit for use in extracting DNA from forensic crime stains. The first stage of the evaluation was to test the sensitivity of the sbeadex® forensic kit at recovering DNA from blood and saliva dilution series’ processed on a liquid handling robotic workstation. It is demonstrated that the sbeadex® forensic kit is suitable for use on forensic crime stain samples where there is only a low level of DNA deposited.

In this application note the performance of the sbeadex® PACE kit is demonstrated. The protocol for recovering DNA from buccal swabs is shown to be in line with the PACE DNA team’s technical standards for loading DNA profiles to the UK National DNA Database is acceptable. The Tecan Freedom Evo extraction system within the PACE laboratory can be used to run the sbeadex® PACE protocol without any hardware modifications.

This application note describes a method for the high throughput extraction of plasmid DNA from bacterial cultures using the sbeadex® plasmid kit from LGC Genomics. The given protocol can be used for a wide range of plasmids/bacterial strains without adaptation, or can be customized using the KingFisher Software.

New approaches in forensic DNA typing technology mostly deal with the analysis of degraded and low copy number DNA with improvements being achieved by modifying PCR processes e.g. increase in the number of PCR cycles, reduction in reaction volume or the use of mini-STRs. In this application note low level DNA samples (artificial and routine casework) were amplified and subjected to two different PCR purification methods and the results compared.

The HIVE system is changing how samples can be stored for single-cell sequencing, opening up its potential as a technique to many more research studies